Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,313,739 (C)5→4 coding (252/2673 nt) rcsD → phosphotransfer intermediate protein in two‑component regulatory system with RcsBC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,313,7350C.100.0% 165.7 / NA 38coding (248/2673 nt)rcsDphosphotransfer intermediate protein in two‑component regulatory system with RcsBC
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (22/16);  total (22/16)

CGCCGTTAACGCCTGGCTGAAAGATAAAAGTTACCAGATTGTCGACATTACCCATGCTATCCAAAAGCGCGTCGATAACTGGCGTTACGTGACCTGGCAGATCTACGACAACATTGCCGCGACGACCTCCCCCTCCTCCGGCGAAGGTTTACAAGAGACGCGCCTGAAACAGGATGTCTACTATCTGGAGAAACCACGCCGCAAAACGGAAGCGTTAATCTTTGGCTCTCACGACAACTCAACGCTTGAGATG  >  NC_000913/2313607‑2313859
                                                                                                                                |                                                                                                                            
cgcCGTTAACGCCTGGCTGAAAGATAAAAGTTACCAGATTGTCGACATTACCCATGCTATCCAAAAGCGCGTCGATAACTGGCGTTACGTGACCTGGCAGATCTACGACAACATTGCCGCGACGACCTCCCCTCCTCCg                                                                                                                   >  1:228716/1‑139 (MQ=255)
  ccGTTAACGCCTGGCTGAAAGATAAAAGTTACCAGATTGTCGACATTACCCATGCTATCCAAAAGCGCGTCGATAACTGGCGTTACGTGACCTGGCAGATCTACGACAACATTGCCGCGACGACCTCCCCTCCTCCGGc                                                                                                                 <  1:27679/139‑1 (MQ=255)
               gCTGAAAGATAAAAGTTACCAGATTGTCGACATTACCCATGCTATCCAAAAGCGCGTCGATAACTGGCGTTACGTGACCTGGCAGATCTACGACAACATTGCCGCGACGACCTCCCCTCCTCCGGCGAAGGTTTACAag                                                                                                    >  1:586473/1‑139 (MQ=255)
                         aaaaGTTACCAGATTGTCGACATTACCCATGCTATCCAAAAGCGCGTCGATAACTGGCGTTACGTGACCTGGCAGATCTACGACAACATTGCCGCGACGACCTCCCCTCCTCCGGc                                                                                                                 <  2:842099/116‑1 (MQ=255)
                         aaaaGTTACCAGATTGTCGACATTACCCATGCTATCCAAAAGCGCGTCGATAACTGGCGTTACGTGACCTGGCAGATCTACGACAACATTGCCGCGACGACCTCCCCTCCTCCGGc                                                                                                                 <  2:599638/116‑1 (MQ=255)
                         aaaaGTTACCAGATTGTCGACATTACCCATGCTATCCAAAAGCGCGTCGATAACTGGCGTTACGTGACCTGGCAGATCTACGACAACATTGCCGCGACGACCTCCCCTCCTCCGGc                                                                                                                 >  1:599638/1‑116 (MQ=255)
                         aaaaGTTACCAGATTGTCGACATTACCCATGCTATCCAAAAGCGCGTCGATAACTGGCGTTACGTGACCTGGCAGATCTACGACAACATTGCCGCGACGACCTCCCCTCCTCCGGc                                                                                                                 >  1:842099/1‑116 (MQ=255)
                          aaaGTTACCAGATTGTCGACATTACCCATGCTATCCAAAAGCGCGTCGATAACTGGCGTTACGTGACCTGGCAGATCTACGACAACATTGCCGCGACGACCTCCCCTCCTCCGGCGAAGGTTTACAAGAGACGCGCCTg                                                                                         >  2:681469/1‑139 (MQ=255)
                                     aTTGTCGACATTACCCATGCTATCCAAAAGCGCGTCGATAACTGGCGTTACGTGACCTGGCAGATCTACGACAACATTGCCGCGACGACCTCCCCTCCTCCGGCGAAg                                                                                                             <  2:563919/108‑1 (MQ=255)
                                     aTTGTCGACATTACCCATGCTATCCAAAAGCGCGTCGATAACTGGCGTTACGTGACCTGGCAGATCTACGACAACATTGCCGCGACGACCTCCCCTCCTCCGGCGAAg                                                                                                             >  1:563919/1‑108 (MQ=255)
                                     aTTGTCGACATTACCCATGCTATCCAAAAGCGCGTCGATAACTGGCGTTACGTGACCTGGCAGATCTACGACAACATTGCCGCGACGACCTCCCCTCCTCCGGCGAAGGTTTACAAGAGACGCGCCTGAAACAGGATGt                                                                              <  1:681469/139‑1 (MQ=255)
                                                       gCTATCCAAAAGCGCGTCGATAACTGGCGTTACGTGACCTGGCAGATCTACGACAACATTGCCGCGACGACCTCCCCTCCTCCGGCGAAGGTTTACAAGAGACGCGCCTGAAACAGGATGTCTACTATCTGGAGAAAcc                                                            >  2:501282/1‑139 (MQ=255)
                                                             cAAAAGCGCGTCGATAACTGGCGTTACGTGACCTGGCAGATCTACGACAACATTGCCGCGACGACCTCCCCTCCTCCGGc                                                                                                                 <  2:816301/80‑1 (MQ=255)
                                                             cAAAAGCGCGTCGATAACTGGCGTTACGTGACCTGGCAGATCTACGACAACATTGCCGCGACGACCTCCCCTCCTCCGGc                                                                                                                 >  1:816301/1‑80 (MQ=255)
                                                               aaaGCGCGTCGATAACTGGCGTTACGTGACCTGGCAGATCTACGACAACATTGCCGCGACGACCTCCCCTCCTCCGGCGAAGGTTTACAAGAGACGCGCCTGAAACAGGATGTCTACTATCTGGAGAAACCACGCCGCa                                                    <  2:228716/139‑1 (MQ=255)
                                                                aaGCGCGTCGATAACTGGCGTTACGTGACCTGGCAGATCTACGACAACATTGCCGCGACGACCTCCCCTCCTCCGGCGAAGGTTTACAAGAGACGCGCCTGAAACAGGATGTCTACTATCTGGAGAAACCACGCCGCaa                                                   >  2:755224/1‑139 (MQ=255)
                                                                    gcgTCGATAACTGGCGTTACGTGACCTGGCAGATCTACGACAACATTGCCGCGACGACCTCCCCTCCTCCGGCGAAGGTTTACAAGAGACGCGCCTGAAACAGGATGTCACTATCTGGAGAAACCACGCCGCAAAACgg                                              <  1:352516/139‑1 (MQ=255)
                                                                     cgTCGATAACTGGCGTTACGTGACCTGGCAGATCTACGACAACATTGCCGCGACGACCTCCCCTCCTCCGGCGAAGGTTTACAAGAGACGCGCCTGAAACAGGATGTCTACTATCTGGAGAAACCACGCCGCAAAACgg                                              >  2:689432/1‑139 (MQ=255)
                                                                     cgTCGATAACTGGCGTTACGTGACCTGGCAGATCTACGACAACATTGCCGCGACGACCTCCCCTCCTCCGGCGAAGGTTTACAAGAGACGCGCCTGAAACAGGATGTCTACTATCTGGAGAAACCACGCCGCAAAACgg                                              >  1:495203/1‑139 (MQ=255)
                                                                      gTCGATAACTGGCGTTACGTGACCTGGCAGATCTACGACAACATTGCCGCGACGACCTCCCCTCCTCCGGCGAAGGTTTACAAGAGACGCGCCTGAAACAGGATGTCTACTATCTGGAGAAACCACGCCGCAAAACGGa                                             <  1:436871/139‑1 (MQ=255)
                                                                                  cGTTACGTGACCTGGCAGATCTACGACAACATTGCCGCGACGACCTCCCCTCCTCCGGCGAAGGTTTACAAGAGACGCGCCTGAAACAGGATGTcta                                                                           >  1:620258/1‑97 (MQ=255)
                                                                                  cGTTACGTGACCTGGCAGATCTACGACAACATTGCCGCGACGACCTCCCCTCCTCCGGCGAAGGTTTACAAGAGACGCGCCTGAAACAGGATGTcta                                                                           <  2:620258/97‑1 (MQ=255)
                                                                                           aCCTGGCAGATCTACGACAACATTGCCGCGACGACCTCCCCTCCTCCGGCGAAGGTTTACAAGAGACGCGCCTGAAACAGGATGTCTACTATCTGGAGAAACCACGCCGCAAAACGGAAGCGTTAATCTTTGGCTCTCa                        >  2:388941/1‑139 (MQ=255)
                                                                                                   gATCTACGACAACATTGCCGCGACGACCTCCCGTCCTCCGGCGAAGGTTTACAAGAGACGCGCCTGAAAc                                                                                     <  1:560924/70‑1 (MQ=255)
                                                                                                   gATCTACGACAACATCGCCGCGACGACCTCCCCTCCTCCGGCGAAGGTTTACAAGAGACGCGCCTGAAAc                                                                                     >  2:560924/1‑70 (MQ=255)
                                                                                                      cTACGACAACATTGCCGCGACGACCTCCCCTCCTCCGGCGAAGGTTTACAAGAGACGCGCCTGAAACAGGATGTCTACTATCTGGAGAAACCACGCCGCAAAACGGAAGCGTTAATCTTTGGCTCTCACGACAACTCaa             >  2:460706/1‑139 (MQ=255)
                                                                                                              acaTTGCCGCGACGACCTCCCCTCCTCCGGCGAAGGTTTACAAGAGACGCGCCTGAAACAGGAt                                                                                <  1:687641/64‑1 (MQ=255)
                                                                                                              acaTTGCCGCGACGACCTCCCCTCCTCCGGCGAAGGTTTACAAGAGACGCGCCTGAAACAGGAt                                                                                >  2:687641/1‑64 (MQ=255)
                                                                                                              acaTTGCCGCGACGACCTCCCCTCCTCCGGCGAAGGTTTACAAGAGACGCGCCTGAAACAGGATGTcta                                                                           >  2:342745/1‑69 (MQ=255)
                                                                                                              acaTTGCCGCGACGACCTCCCCTCCTCCGGCGAAGGTTTACAAGAGACGCGCCTGAAACAGGATGTcta                                                                           <  1:342745/69‑1 (MQ=255)
                                                                                                               caTTGCCGCGACGACCTCCCCTCCTCCGGCGAAGGTTTACAAGAGAcgc                                                                                              >  2:234989/1‑49 (MQ=255)
                                                                                                               caTTGCCGCGACGACCTCCCCTCCTCCGGCGAAGGTTTACAAGAGAcgc                                                                                              <  1:234989/49‑1 (MQ=255)
                                                                                                               caTTGCCGCGACGACCTCCCCTCCTCCGGCGAAGGTTTACAAGAGACGCGCCTGAAACAGGATGTCTACTATCTGGAGAAACCAcgccg                                                      <  2:70780/89‑1 (MQ=255)
                                                                                                               caTTGCCGCGACGACCTCCCCTCCTCCGGCGAAGGTTTACAAGAGACGCGCCTGAAACAGGATGTCTACTATCTGGAGAAACCAcgccg                                                      >  1:70780/1‑89 (MQ=255)
                                                                                                               caTTGCCGCGACGACCTCCCCTCCTCCGGCGAAGGTTTACAAGAGACGCGCCTGAAACAGGATGTCTACTATCTGGAGAAACCACGCCGCAAAACGGAAGCGTTAATCTTTGGCTCTCACGACAACTCAACGCTTgaga    >  2:170656/1‑139 (MQ=255)
                                                                                                                 ttGCCGCGACGACCTCCCCTCCTCCGGCGAAGGTTTACAAGAGACGCGCCTGAAACAGGATGTCTACTATCTGGAGAAACCACGCCGCAAAACGGAAGCGTTAATCTTTGGCTCTCACGACAACTCAACGCTTGAGATg  >  2:79695/1‑139 (MQ=255)
                                                                                                                         acgacCTCCCCTCCTCCGGCGAAGGTTTACAAGAGACGCGCCTGAAACAGGATGTCTACTAt                                                                       <  1:437498/62‑1 (MQ=255)
                                                                                                                         acgacCTCCCCTCCTCCGGCGAAGGTTTACAAGAGACGCGCCTGAAACAGGATGTCTACTAt                                                                       >  2:437498/1‑62 (MQ=255)
                                                                                                                                |                                                                                                                            
CGCCGTTAACGCCTGGCTGAAAGATAAAAGTTACCAGATTGTCGACATTACCCATGCTATCCAAAAGCGCGTCGATAACTGGCGTTACGTGACCTGGCAGATCTACGACAACATTGCCGCGACGACCTCCCCCTCCTCCGGCGAAGGTTTACAAGAGACGCGCCTGAAACAGGATGTCTACTATCTGGAGAAACCACGCCGCAAAACGGAAGCGTTAATCTTTGGCTCTCACGACAACTCAACGCTTGAGATG  >  NC_000913/2313607‑2313859

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: