Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,824,496 Δ1 bp coding (1081/1086 nt) mltB ← membrane‑bound lytic murein transglycosylase B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,824,4960G.100.0% 55.8 / NA 14coding (1081/1086 nt)mltBmembrane‑bound lytic murein transglycosylase B
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (7/7);  total (7/7)

TCGTCAGGAAGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGCTACTGTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCTGTGGTTGTAACGGGTGATGGTGTAGAAGTTCGGCAGACCGTACCAGTACTGGTAGCCGGTGCCAACAT  >  NC_000913/2824393‑2824624
                                                                                                         |                                                                                                                                
tCGTCAGGAAGCTGGTGCTGGCCTTGCAATTTTACGAGGAGGATTCAGAAACAAGCTGATTAGCCAGAGGGAAGCTCACGCTCCCCCCCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGcc                                                                                               >  2:419058/1‑139 (MQ=255)
      ggAAGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCg                                                                                         <  1:742800/139‑1 (MQ=255)
               tgtTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGt                                                                                >  2:653082/1‑139 (MQ=255)
                         gCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCCTCTTGTAAATAGTTACTTACTCgcgccagcgcc                                                                                                                  <  1:142769/95‑1 (MQ=255)
                         gCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCCTCTTGTAAATAGTTACTTACTCgcgccagcgcc                                                                                                                  >  2:142769/1‑95 (MQ=255)
                            aTTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCtgtg                                                                   <  1:713897/139‑1 (MQ=255)
                                          aTTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCTGTGGTTGTAACGGGTGa                                                     <  1:414363/139‑1 (MQ=255)
                                                               ccAGAGGGAAGCTCACGCCCCCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCTGTGGTTGTAACGGGTGATGGTGTAGAAGTTCGGCAGAc                                <  1:653082/139‑1 (MQ=255)
                                                               ccAGAGGGAAGCTCACGCCCCCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCTGTGGTTGTAACGGGTGATGGTGTAGAAGTTCGGCAGAc                                >  1:815787/1‑139 (MQ=255)
                                                                    gggAAGCTCACGCCCCCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACGGCTTGTCCGAACTGCCAGACCGCCATTGCGTAATGGGTGCTGTGGTTGTAACGGGTGATGGTGTAGAAGTTCGGCAGACCGTAc                           <  1:615917/139‑1 (MQ=255)
                                                                        aGCTCACGCCCCCCCTCTTGTAAATAGTTACTTACTCgcgc                                                                                                                         <  1:157305/41‑1 (MQ=255)
                                                                        aGCTCACGCCCCCCCTCTTGTAAATAGTTACTTACTCgcgc                                                                                                                         >  2:157305/1‑41 (MQ=255)
                                                                                            gTAAATAGTTACTTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCTGTGGTTGTAACGGGTGATGGTGTAGAAGTTCGGCAGACCGTACCAGTACTGGTAGCCGGTGCCAAc    >  2:32784/1‑139 (MQ=255)
                                                                                              aaaTAGTTACTTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCTGTGGTTGTAACGGGTGATGGTGTAGAAGTTCGGCAGACCGTACCAGTACTGGTAGCCGGTGCCAACAt  >  2:309394/1‑139 (MQ=255)
                                                                                                         |                                                                                                                                
TCGTCAGGAAGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGCTACTGTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCTGTGGTTGTAACGGGTGATGGTGTAGAAGTTCGGCAGACCGTACCAGTACTGGTAGCCGGTGCCAACAT  >  NC_000913/2824393‑2824624

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: