Predicted mutation | ||||||
---|---|---|---|---|---|---|
evidence | seq id | position | mutation | annotation | gene | description |
RA | NC_000913 | 321,564 | (G)7→6 | intergenic (+483/‑44) | rclR → / → ykgE | reactive chlorine species (RCS)‑specific activator of the rcl genes/cysteine‑rich LutA family protein, putative electron transport chain YkgEFG component |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 321,558 | 0 | G | . | 96.8% | 127.2 / ‑2.1 | 31 | intergenic (+477/‑50) | rclR/ykgE | reactive chlorine species (RCS)‑specific activator of the rcl genes/cysteine‑rich LutA family protein, putative electron transport chain YkgEFG component |
Reads supporting (aligned to +/- strand): ref base G (1/0); new base . (14/16); total (15/16) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 4.84e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.71e-01 |
CCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGAAATCAAGAA > NC_000913/321425‑321659 | ccGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTgggggg < 1:422269/139‑1 (MQ=255) cGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTgggggga < 2:157699/139‑2 (MQ=255) aGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGT‑GGGGGGAAACATCGGGCATAAAt > 2:799119/1‑139 (MQ=255) gACGTTTGGATGAAAGATTATCATTTGTCATAGAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTaa < 2:316278/139‑1 (MQ=255) gACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGCGGTATGTTGCTAGCCAATTAAAAAAGAACGCAATATTTATTGATGATTGATCCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTaa > 2:70011/1‑139 (MQ=255) ttGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTg > 2:580689/1‑99 (MQ=255) ttGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTg < 1:580689/99‑1 (MQ=255) tGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAAt > 2:400681/1‑139 (MQ=255) gTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCa < 2:602786/105‑1 (MQ=255) gTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCa > 1:602786/1‑105 (MQ=255) aTACAAATGAGGGGTGGTATGTTGCTAGCCAAGTAAAAAAGAACGCCATATGTATTGATGATTGATCCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTACTTGTGATTGTCAATTCCt < 2:487098/139‑1 (MQ=255) cAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATg > 2:89284/1‑101 (MQ=255) cAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATg < 1:89284/101‑1 (MQ=255) tGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCAc < 1:400681/139‑1 (MQ=255) gggTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCAc > 1:423950/1‑135 (MQ=255) gggTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCAc < 2:423950/135‑1 (MQ=255) tggtATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGT‑GGGGGGAAACATCGGGCATAAAt < 1:658033/83‑1 (MQ=255) tggtATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGT‑GGGGGGAAACATCGGGCATAAAt > 2:658033/1‑83 (MQ=255) tggtATGTTGCTAGCCAATTAAAAAAGAACACCATATTTATTGATGATTGATCCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTaa < 1:337191/96‑1 (MQ=255) tggtATGTTGCTAGCCAATTAAAAAAGAACACCATATTTATTGATGATTGATCCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTaa > 2:337191/1‑96 (MQ=255) gtATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTggggggaaa < 2:535462/67‑4 (MQ=255) gtATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGaaa > 1:535462/1‑67 (MQ=255) gtATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCAcc > 2:309732/1‑131 (MQ=255) gtATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCAcc < 1:309732/131‑1 (MQ=255) aGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCa < 2:304581/119‑1 (MQ=255) aGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCa > 1:304581/1‑119 (MQ=255) aGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTg < 1:325159/139‑1 (MQ=255) aaaaGAACACCATTTTTATTGATGATTGATCACCCGGT‑GGGGGGAAACAGCGAGCATAAATGGGCATGAAGTATTGGCGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTCACGCCCTGAAATCAAGaa < 2:754016/139‑1 (MQ=255) tgattgatCCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCa > 2:443291/1‑44 (MQ=255) tgattgatCCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCa < 1:443291/44‑1 (MQ=255) atCCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTaa < 1:671786/46‑1 (MQ=255) atCCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTaa > 2:671786/1‑46 (MQ=255) atCCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTAt > 1:257116/1‑54 (MQ=255) atCCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTAt < 2:257116/54‑1 (MQ=255) atCCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCAc < 2:328987/82‑1 (MQ=255) atCCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCAc > 1:328987/1‑82 (MQ=255) | CCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGAAATCAAGAA > NC_000913/321425‑321659 |
Alignment Legend |
---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |