Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,447,869 Δ1 bp coding (3758/3780 nt) tamB → translocation and assembly module for autotransporter export, inner membrane subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,447,8670T.100.0% 288.8 / NA 65coding (3756/3780 nt)tamBtranslocation and assembly module for autotransporter export, inner membrane subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (35/30);  total (35/30)

CAAGTGAAATACGGCGTGGGTATATTTGACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTTGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAG  >  NC_000913/4447754‑4447995
                                                                                                                 |                                                                                                                                
cAAGTGAAATACGGCGTGGGTATATTTGACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTaaca                                                                                                        >  1:740755/1‑136 (MQ=255)
cAAGTGAAATACGGCGTGGGTATATTTGACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACa                                                                                                        >  2:644738/1‑139 (MQ=255)
               gTGGGTATATTTGACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTc                                                                                         >  1:664880/1‑139 (MQ=255)
                  ggTATATTTGACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCg                                                                                                                  <  2:38660/111‑1 (MQ=255)
                  ggTATATTTGACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCg                                                                                                                  >  1:38660/1‑111 (MQ=255)
                          tGACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGttt                                                                              <  2:100868/139‑1 (MQ=255)
                            aCTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCg                                                                                                   >  2:368292/1‑116 (MQ=255)
                            aCTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCg                                                                                                   <  1:368292/116‑1 (MQ=255)
                             ctctATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACg                                                                           <  2:291349/139‑1 (MQ=255)
                                 ataGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCcac                                                                       >  1:582498/1‑139 (MQ=255)
                                          cTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGAttg                                                                                                                                >  2:50192/1‑73 (MQ=255)
                                          cTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGAttg                                                                                                                                <  1:50192/74‑2 (MQ=255)
                                          cTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGc                                                              >  1:480118/1‑139 (MQ=255)
                                          cTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAgg                                                               <  2:93023/138‑1 (MQ=255)
                                          cTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAgg                                                               >  1:93023/1‑138 (MQ=255)
                                          cTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGc                                                              >  2:265666/1‑139 (MQ=255)
                                                ttacgttaTCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGt                                                        >  1:60401/1‑139 (MQ=255)
                                                  acgttaTCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACgg                                                                                    <  1:499014/109‑1 (MQ=255)
                                                  acgttaTCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACgg                                                                                    >  2:499014/1‑109 (MQ=255)
                                                       aTCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGa                                                 >  2:200357/1‑139 (MQ=255)
                                                           ccTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGAttg                                                                                                                                <  1:752603/57‑2 (MQ=255)
                                                           ccTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGAttg                                                                                                                                >  2:752603/1‑56 (MQ=255)
                                                              gATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCaa                                          <  1:615334/139‑1 (MQ=255)
                                                               aTGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCaa                                          >  1:9595/1‑138 (MQ=255)
                                                               aTGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCaa                                          <  2:9595/138‑1 (MQ=255)
                                                                  ccTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGcc                                      >  2:609614/1‑139 (MQ=255)
                                                                    tAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACACCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCa                                    <  2:664880/139‑1 (MQ=255)
                                                                           tatCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGAt                                                <  2:235175/120‑1 (MQ=255)
                                                                           tatCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGAt                                                >  1:235175/1‑120 (MQ=255)
                                                                           tatCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTg                             >  1:271194/1‑139 (MQ=255)
                                                                            atCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTgg                            >  2:357795/1‑139 (MQ=255)
                                                                              cTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGc                          <  1:609614/139‑1 (MQ=255)
                                                                              cTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGc                          <  2:582498/139‑1 (MQ=255)
                                                                                ggAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGa                        >  2:799504/1‑139 (MQ=255)
                                                                                 gAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGc                                                                          >  2:152778/1‑88 (MQ=255)
                                                                                 gAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCAc                                                     <  1:442393/109‑1 (MQ=255)
                                                                                 gAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCAc                                                     >  2:442393/1‑109 (MQ=255)
                                                                                 gAAGCCGTGTCTGGTGTAGACCAGGAACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGc                                                                          <  1:152778/88‑1 (MQ=255)
                                                                                  aaGCCGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTa                                                                                       >  1:827234/1‑74 (MQ=255)
                                                                                  aaGCCGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTa                                                                                       <  2:827234/74‑1 (MQ=255)
                                                                                  aaGCCGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCaa                                                            <  1:194384/101‑1 (MQ=255)
                                                                                  aaGCCGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCaa                                                            >  2:194384/1‑101 (MQ=255)
                                                                                      cGTGTCTGGTGTAGACCAGGCACTGGATTGTTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTc                                                                                         >  1:758476/1‑68 (MQ=255)
                                                                                      cGTGTCTGGTGTAGACCAGGCACTGGATTGTTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTc                                                                                         <  2:758476/68‑1 (MQ=255)
                                                                                        tgtCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCa                   >  2:209563/1‑136 (MQ=255)
                                                                                        tgtCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCa                   <  1:209563/136‑1 (MQ=255)
                                                                                                    aCCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACg                                                                                     <  2:243289/58‑1 (MQ=255)
                                                                                                    aCCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACg                                                                                     >  1:243289/1‑58 (MQ=255)
                                                                                                    aCCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTAcc    >  1:443348/1‑139 (MQ=255)
                                                                                                     ccAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCa   <  1:357795/139‑1 (MQ=255)
                                                                                                      cAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAg  <  2:686747/139‑1 (MQ=255)
                                                                                                        ggCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAatat                                                                                              >  2:397152/1‑45 (MQ=255)
                                                                                                        ggCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAatat                                                                                              <  1:397152/45‑1 (MQ=255)
                                                                                                        ggCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGc                                                                          >  1:235459/1‑65 (MQ=255)
                                                                                                        ggCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGc                                                                          <  2:235459/65‑1 (MQ=255)
                                                                                                        ggCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGcta                                                            >  1:837696/1‑77 (MQ=255)
                                                                                                        ggCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCaa                                                            <  2:837696/79‑1 (MQ=255)
                                                                                                        ggCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCaa                                                            <  1:563794/79‑1 (MQ=255)
                                                                                                        ggCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCaa                                                            >  2:563794/1‑79 (MQ=255)
                                                                                                        ggCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTggg                           >  2:743405/1‑112 (MQ=255)
                                                                                                        ggCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTggg                           <  1:743405/112‑1 (MQ=255)
                                                                                                        ggCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCa   <  2:381289/136‑1 (MQ=255)
                                                                                                        ggCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCa   >  1:381289/1‑136 (MQ=255)
                                                                                                         gCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTa                                                                                       <  1:268842/51‑1 (MQ=255)
                                                                                                         gCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTa                                                                                       >  2:268842/1‑51 (MQ=255)
                                                                                                         gCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGt                                                        <  2:130113/82‑1 (MQ=255)
                                                                                                         gCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGt                                                        >  1:130113/1‑82 (MQ=255)
                                                                                                         gCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGa            <  2:148895/126‑1 (MQ=255)
                                                                                                         gCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGa            >  1:148895/1‑126 (MQ=255)
                                                                                                                 |                                                                                                                                
CAAGTGAAATACGGCGTGGGTATATTTGACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTTGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAG  >  NC_000913/4447754‑4447995

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: