Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,157,712 (C)6→7 coding (63/927 nt) yqhG → DUF3828 family putative periplasmic protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,157,7061.C100.0% 107.0 / NA 30Q19H (CAG→CAC) yqhGDUF3828 family putative periplasmic protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (14/16);  total (14/16)

ATCATGCAGAGCCGGAAGCTCTTAAAAGAACAACTCATCTATATCCGGGATAAACGCAACGGAGAGGTGAAAAACAGATGAAAATAATACTTCTGTTTTTAGCAGCCCTGGCAAGTTTTACCGTACACGCACAG‑CCCCCCTCACAGACCGTAGAACAAACAGTCCGGCATATTTATCAGAACTATAAATCAGATGCCACTGCCCCTTATTTTGGTGAAACCGGAGAGCGGGCGATAACTTCTGC  >  NC_000913/3157573‑3157816
                                                                                                                                      |                                                                                                              
atcatGCAGAGCCGGAAGCTCTTAAAAGAACAACTCATCTATATCCGGGATAAACGCAACGGAGAGGTGAAAAACAGATGAAAATAATACTTCTGTTTTTAGCAGCCCTGGCAAGTTTTACCGTACACGCACAG‑ccccc                                                                                                           >  1:606549/1‑139 (MQ=255)
         agCCGGAAGCTCTTAAAAGAACAACTCATCTATATCCGGGATAAACGCAACGGAGAGGTGAAAAACAGATGAAAATAATACTTCTGTTTTTAGCAGCCCTGGCAAGTTTTACCGTACACGCACAGCCCCCCCTCACAGa                                                                                                   >  1:215400/1‑139 (MQ=255)
           ccGGAAGCTCTTAAAAGAACAACTCATCTATATCCGGGATAAACGCAACGGAGAGGTGAAAAACAGATGAAAATAATACTTCTGTTTTTAGCAGCCCTGGCAAGTTTTACCGTACACGCACAGCCCCCCCTCACAGAcc                                                                                                 <  2:215400/139‑1 (MQ=255)
                     ttAAAAGAACAACTCATCTATATCCGGGATAAACGCAACGGAGAGGTGAAAAACAGATGAAAATAATACTTCTGTTTTTAGCAGCCCTGGCAAGTTTTACCGTACACGCACAGCCCCCCCTCACAGACCGTAGaacaaa                                                                                       >  2:775990/1‑139 (MQ=255)
                            aacaacTCATCTATATCCGGGATAAACGCAACGGAGAGGTGAAAAACAGATGAAAATAATACTTCTGTTTTTAGCAGCCCTGGCAAGTTTTACCGTACACGCACAGCCCCCCCTCACAGACCGTAGAACAAACAGTCCg                                                                                <  1:775990/139‑1 (MQ=255)
                            aacaacTCATCTATATCCGGGATAAACGCAACGGAGAGGTGAAAAACAGATGAAAATAATACTTCTGTTTTTAGCAGCCCTGGCAAGTTTTACCGTACACGCACAGCCCCCCCTCACAGACCGTAGAACAAACAGTCCg                                                                                >  2:289721/1‑139 (MQ=255)
                             acaacTCATCTATATCCGGGATAAACGCAACGGAGAGGTGAAAAACAGATGAAAATAATACTTCTGTTTTTAGCAGCCCTGGCAAGTTTTACCGTACACGCACAGCCCCCCCTCACAGACCGTAGAACAAACAGTCCgg                                                                               >  2:305901/1‑139 (MQ=255)
                              caacTCATCTATATCCGGGATAAACGCAACGGAGAGGTGAAAAACAGATGAAAATAATACTTCTGTTTTTAGCAGCCCTGGCAAGTTTTACCGTACACGCACAGCCCCCCCTCACAGACCGTAGAACAAACAGTCCGGc                                                                              <  1:702236/139‑1 (MQ=255)
                               aacTCATCTATATCCGGGATAAACGCAACGGAGAGGTGAAAAACAGATGAAAATAATACTTCTGTTTTTAGCAGCCCTGGCAAGTTTTACCGTACACGCACAg                                                                                                                 <  2:716274/103‑1 (MQ=255)
                               aacTCATCTATATCCGGGATAAACGCAACGGAGAGGTGAAAAACAGATGAAAATAATACTTCTGTTTTTAGCAGCCCTGGCAAGTTTTACCGTACACGCACAg                                                                                                                 >  1:716274/1‑103 (MQ=255)
                                           tCCGGGATAAACGCAACGGAGAGGTGAAAAACAGATGAAAATAATACTTCTGTTTTTAGCAGCCCTGGCAAGTTTTACCGTACACGCACAGCCCCCCCTCACAGACCGTAGAACAAACAGTCCGGCATATTTATCAGaa                                                                 <  2:606549/139‑1 (MQ=255)
                                            ccGGGATAAACGCAACGGAGAGGTGAAAAACAGATGAAAATAATACTTCTGTTTTTAGCAGCCCTGGCAAGTTTTACCGTACACGCACAGCCCCCCCTCACAGACCGTAGAACAAACAGTCCg                                                                                <  2:670115/123‑1 (MQ=255)
                                            ccGGGATAAACGCAACGGAGAGGTGAAAAACAGATGAAAATAATACTTCTGTTTTTAGCAGCCCTGGCAAGTTTTACCGTACACGCACAGCCCCCCCTCACAGACCGTAGAACAAACAGTCCg                                                                                >  1:670115/1‑123 (MQ=255)
                                              gggATAAACGCAACGGAGAGGTGAAAAACAGATGAAAATAATACTTCTGTTTTTAGCAGCCCTGGCAAGTTTTACCGTACACGCACAGCCCCCCCTCACAGAcc                                                                                                 <  1:455156/104‑1 (MQ=255)
                                              gggATAAACGCAACGGAGAGGTGAAAAACAGATGAAAATAATACTTCTGTTTTTAGCAGCCCTGGCAAGTTTTACCGTACACGCACAGCCCCCCCTCACAGAcc                                                                                                 >  2:455156/1‑104 (MQ=255)
                                               ggATAAACGCAACGGAGAGGTGAAAAACAGATGAAAATAATACTTCTGTTTTTAGCAGCCCTGGCAAGTTTTACCGTACACGCACAGCCCCCCCTCACAGACCGTAGAACAAACAGTCCGGCATATTTATCAGAACtat                                                             >  1:663504/1‑139 (MQ=255)
                                                gATAAACGCAACGGAGAGGTGAAAAACAGATGAAAATAATACTTCTGTTTTTAGCAGCCCTGGCAAGTTTTACCGTACACGCACAGCCCCCCCTCACAGACCGTAGaacaaa                                                                                       >  1:389829/1‑112 (MQ=255)
                                                gATAAACGCAACGGAGAGGTGAAAAACAGATGAAAATAATACTTCTGTTTTTAGCAGCCCTGGCAAGTTTTACCGTACACGCACAGCCCCCCCTCACAGACCGTAGaacaaa                                                                                       <  2:389829/112‑1 (MQ=255)
                                                   aaaCGCAACGGAGAGGTGAAAAACAGATGAAAATAATACTTCTGTTTTTAGCAGCCCTGGCAAGTTTTACCGTACACGCACAGCCCCCCCTCACAGACCGTAGAACAAACAGTCCGGCATATTTATCAGAACTATAAAt                                                         >  1:122104/1‑139 (MQ=255)
                                                    aaCGCAACGGAGAGGTGAAAAACAGATGAAAATAATACTTCTGTTTTTAGCAGCCCTGGCAAGTTTTACCGTACACGCACAg                                                                                                                 >  2:98548/1‑82 (MQ=255)
                                                    aaCGCAACGGAGAGGTGAAAAACAGATGAAAATAATACTTCTGTTTTTAGCAGCCCTGGCAAGTTTTACCGTACACGCACAg                                                                                                                 <  1:98548/82‑1 (MQ=255)
                                                         aaCGGAGAGGTGAAAAACAGATGAAAATAATACTTCTGTTTTTAGCAGCCCTGGCAAGTTTTACCGTACACGCACAGCCCCCCCTCACAGa                                                                                                   <  2:88165/91‑1 (MQ=255)
                                                         aaCGGAGAGGTGAAAAACAGATGAAAATAATACCTCTGTTTTTAGCAGCCCTGGCAAGTTTTACCGTACACGCACAGCCCCCCCTCACAGa                                                                                                   >  1:88165/1‑91 (MQ=255)
                                                            ggAGAGGTGAAAAACAGATGAAAATAATACTTCTGTTTTTAGCAGCCCTGGCAAGTTTTACCGTACACGCACAGCCCCCCCTCACAGACCGTAGAACAAACAGTCCGGCATATTTATCAGAACTATAAATCAGATGCCa                                                <  2:663504/139‑1 (MQ=255)
                                                              agagGTGAAAAACAGATGAAAATAATACTTCTGTTTTTAGCAGCCCTGGCAAGTTTTACCGTACACGCACAGCCCCCCCTCACAGACCGTAGAACAAACAGTCCGGCATATTTATCAGAACTATAAATCAGATGCCACt                                              <  1:366040/139‑1 (MQ=255)
                                                                       aaaCAGATGAAAATAATACTTCTGTTTTTAGCAGCCCTGGCAAGTTTTACCGTACATGCACAGCCCCCCCTCACAGACCGTAGAACAAACAGTCCGGCATATTTATCAg                                                                   >  2:319414/1‑109 (MQ=255)
                                                                       aaaCAGATGAAAATAATACTTCTGTTTTTAGCAGCCCTGGCAAGTTTTACCGTACATGCACAGCCCCCCCTCACAGACCGTAGAACAAACAGTCCGGCATATTTATCAg                                                                   <  1:319414/109‑1 (MQ=255)
                                                                            gATGAAAATAATACTTCTGTTTTTAGCAGCCCTGGCAAGTTTTACCGTACACGCACAGCCCCCCCTCACAGACCGTAGAACAAACAGTCCGGCATATTTATCAg                                                                   <  2:581971/104‑1 (MQ=255)
                                                                            gATGAAAATAATACTTCTGTTTTTAGCAGCCCTGGCAAGTTTTACCGTACACGCACAGCCCCCCCTCACAGACCGTAGAACAAACAGTCCGGCATATTTATCAg                                                                   >  1:581971/1‑104 (MQ=255)
                                                                             aTGAAAATAATACTTCTGTTTTTAGCAGCCCTGGCAAGTTTTACCGTACACGCACAG‑ccccccctca                                                                                                      <  2:460501/67‑5 (MQ=255)
                                                                             aTGAAAATAATACTTCTGTTTTTAGCAGCCCTGGCAAGTTTTACCGTACACGCACAG‑ccccccctca                                                                                                      >  1:460501/1‑63 (MQ=255)
                                                                              tGAAAATAATACTTCTGTTTTTAGCAGCCCTGGCAAGTTTTACCGTACACGCACAGCCCCCCCTCACAGACCGTAGAACAAACAGTCCGGCATATTTATCAGAACTATAAATCAGATGCCACTGCCCCTTATTTTGGTg                              <  2:122104/139‑1 (MQ=255)
                                                                                               tttttAGCAGCCCTGGCAAGTTTTACCGTACACGCACAGCCCCCCCTCACAGACCGTAGAACAAACAGTCCg                                                                                >  2:829702/1‑72 (MQ=255)
                                                                                               tttttAGCAGCCCTGGCAAGTTTTACCGTACACGCACAGCCCCCCCTCACAGACCGTAGAACAAACAGTCCg                                                                                <  1:829702/72‑1 (MQ=255)
                                                                                                    agcagcCCTGGCAAGTTTTACCGTACACGCACAG‑ccc                                                                                                             <  1:407590/37‑1 (MQ=255)
                                                                                                    agcagcCCTGGCAAGTTTTACCGTACACGCACAG‑ccc                                                                                                             >  2:407590/1‑37 (MQ=255)
                                                                                                          ccTGGCAAGTTTTACCGTACACGCACAGCCCCCCCTCACAGACCGTAGAACAAACAGTCCCGCATATTTATCAGAACTATAAATCAGATGCCACTGCCCCTTATTTTGGTGAAACCGGAGAGCGGGCGATAACTTCTgc  <  1:289721/139‑1 (MQ=255)
                                                                                                              gCAAGTTTTACCGTACACGCACAGCCCCCCCTCACAGACCGTAGAACAAACAGTCCGGCATATTTATCAGAACTATAAATCAGATGCCACTGCCCCTTATTTTGGTGAAACCGGAGAGCgg                >  2:756452/1‑121 (MQ=255)
                                                                                                              gCAAGTTTTACCGTACACGCACAGCCCCCCCTCACAGACCGTAGAACAAACAGTCCGGCATATTTATCAGAACTATAAATCAGATGCCACTGCCCCTTATTTTGGTGAAACCGGAGAGCgg                <  1:756452/121‑1 (MQ=255)
                                                                                                                                      |                                                                                                              
ATCATGCAGAGCCGGAAGCTCTTAAAAGAACAACTCATCTATATCCGGGATAAACGCAACGGAGAGGTGAAAAACAGATGAAAATAATACTTCTGTTTTTAGCAGCCCTGGCAAGTTTTACCGTACACGCACAG‑CCCCCCTCACAGACCGTAGAACAAACAGTCCGGCATATTTATCAGAACTATAAATCAGATGCCACTGCCCCTTATTTTGGTGAAACCGGAGAGCGGGCGATAACTTCTGC  >  NC_000913/3157573‑3157816

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑