| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,240,984 | (G)7→9 | coding (1041/1245 nt) | sstT → | sodium:serine/threonine symporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,240,977 | 1 | . | G | 97.3% | 129.1 / ‑3.1 | 37 | coding (1034/1245 nt) | sstT | sodium:serine/threonine symporter |
| Reads supporting (aligned to +/- strand): ref base . (0/1); new base G (20/16); total (20/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.59e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01 | |||||||||||
| * | NC_000913 | 3,240,977 | 2 | . | G | 91.9% | 118.9 / 4.0 | 37 | coding (1034/1245 nt) | sstT | sodium:serine/threonine symporter |
| Reads supporting (aligned to +/- strand): ref base . (1/2); new base G (19/15); total (20/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.84e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.58e-01 | |||||||||||
TGGCGGGCGCAGCAATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGC‑‑GTGGC‑‑GGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGAAA > NC_000913/3240842‑3241096 || tGGCGGGCGCAGCAATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGC‑‑GTGGC‑‑ggg > 1:840997/1‑139 (MQ=255) cgggcgCAGCAATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGC‑‑GTGGC‑‑gggggg > 2:737261/1‑139 (MQ=255) gggcgCAGCAATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGC‑‑GTGGC‑‑ggggggg > 2:307582/1‑139 (MQ=255) tgtTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGC‑‑GTGGCGGGGGGGGGTCTCTGCTGCTGATCCCACTg < 2:840997/139‑1 (MQ=255) tgtTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGC‑‑GTGGCGGGGGGGGGTCTCTGCTGCTGATCCCACTg > 2:817066/1‑139 (MQ=255) tgtTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGC‑‑GTGGCGGGGGGGGGTCTCTGCTGCTGATCCCACTg > 2:223924/1‑139 (MQ=255) ttGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGC‑‑GTGGCGGGGGGGGGTCTCTGCTGCTGATCCCACTGGc > 1:811564/1‑139 (MQ=255) ttGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGC‑‑GTGGCGGGGGGGGGTCTCTGCTGCTGATCCCACTGGc > 2:289113/1‑139 (MQ=255) ttGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGC‑‑GTGGCGGGGGGGGGTCTCTGCTGCTGATCCCACTGGc > 1:646017/1‑139 (MQ=255) ttGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGC‑‑GTGGCGGGGGGGGGTCTCTGCTGCTGATCCCACTGGc < 1:289113/139‑1 (MQ=255) ttGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGC‑‑GTGGCGGGGGGGGGTCTCTGCTGCTGATCCCACTGGc < 1:307582/139‑1 (MQ=255) ctggctgCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGC‑‑GTGGCGGGGGGGGGTCGCTGCTGCTGATCCCACTGGCCTGTaa > 2:818999/1‑139 (MQ=255) gctgCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGC‑‑GTGGCGGGGGGGGGACTCTGCTGCTGATCCCACTGGCCTGTAatat > 1:459958/1‑139 (MQ=255) gggTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGC‑‑GTGGCGGGGGGGGGTCTCTGCTGCTGa < 1:412683/103‑1 (MQ=255) gggTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGC‑‑GTGGCGGGGGGGGGTCTCTGCTGCTGa > 2:412683/1‑103 (MQ=255) ccGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGC‑‑GTGGCGGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGc < 2:811564/139‑1 (MQ=255) tCGATCTGCCCACGGCGCTGCTGTTGGGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGC‑‑GTGGCGGGGGGGGGTCTCTGCTGCTGATCCCa > 2:666802/1‑97 (MQ=255) tCGATCTGCCCACGGCGCTGCTGTTGGGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGC‑‑GTGGCGGGGGGGGGTCTCTGCTGCTGATCCCa < 1:666802/97‑1 (MQ=255) tCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGC‑‑GTGGCGGGGGGGGGTCTCTGCTGCTGATCCCa > 1:427569/1‑97 (MQ=255) tCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGC‑‑GTGGCGGGGGGGGGTCTCTGCTGCTGATCCCa < 2:427569/97‑1 (MQ=255) tCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGC‑‑GTGGCGGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATg > 1:19586/1‑139 (MQ=255) gATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGC‑‑GTGGCGGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTa > 1:382130/1‑117 (MQ=255) gATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGC‑‑GTGGCGGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTa < 2:382130/117‑1 (MQ=255) gctgctGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGC‑‑GTGGCGGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCg < 1:223924/139‑1 (MQ=255) gAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGC‑‑GTGGCGGGGGGGGGTCTCTGCTGCTGa < 1:464262/67‑1 (MQ=255) gAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGC‑‑GTGGCGGGGGGGGGTCTCTGCTGCTGa > 2:464262/1‑67 (MQ=255) gAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGC‑‑GTGGCGGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTatca > 2:185609/1‑139 (MQ=255) cGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGC‑‑GTGGCGGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCg > 1:447334/1‑139 (MQ=255) cGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGC‑‑GTGGCGGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCg < 1:737261/139‑1 (MQ=255) ggtggCTTCTCTGTGTGCCTGTGGCGCATCCGGC‑‑GTGGCGGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATTTGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCg < 1:185609/139‑1 (MQ=255) ttttgtttccttttgGCGCACCCGGCGGGGGGG‑‑GGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAg < 1:610285/126‑1 (MQ=255) gtgtgCCTGTGGCGCATCCGGC‑‑GTGGCGGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCg < 1:212056/127‑1 (MQ=255) gtgtgCCTGTGGCGCATCCGGC‑‑GTGGCGGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCg > 2:212056/1‑127 (MQ=255) tgGCGCATCCGGC‑‑GTGGCGGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGaaa > 1:363032/1‑139 (MQ=255) gcgcATCCGGC‑‑GTGGCGGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTAtt > 2:388059/1‑67 (MQ=255) gcgcATCCGGC‑‑GTGGCGGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTAtt < 1:388059/67‑1 (MQ=255) tCCGGC‑‑GTGGCGGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGa > 1:710600/1‑66 (MQ=255) tCCGGC‑‑GTGGCGGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGa < 2:710600/66‑1 (MQ=255) tCCGGC‑‑GTGGCG‑GGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCg > 1:657055/1‑56 (MQ=255) tCCGGC‑‑GTGGCG‑GGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCg < 2:657055/56‑1 (MQ=255) || TGGCGGGCGCAGCAATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGC‑‑GTGGC‑‑GGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGAAA > NC_000913/3240842‑3241096 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |