Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,709,561	(C)6→7	coding (915/1692 nt)	eptB ←	KDO phosphoethanolamine transferase, Ca(2+)‑inducible

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,709,555	1	.	C	100.0%	159.3 / NA	46	G307G (GGC→GGG)	eptB	KDO phosphoethanolamine transferase, Ca(2+)‑inducible
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (21/25);  total (21/25)

GCAATGTTGTCCGCCATCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCGTGGTATTACGCTCATAGCCGAAAATACC  >  NC_000913/3709418‑3709686                                                                                                                                           |                                                                                                                                    gCAATGTTGTCCGCCATCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCgcg‑c                                                                                                                                    >  2:434288/1‑139 (MQ=255)    aTGTTGTCCGCCATCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑cccc                                                                                                                                 >  1:747393/1‑139 (MQ=255)      gttgtCCGCCATCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑cccccc                                                                                                                               <  2:454701/139‑1 (MQ=255)      gttgtCCGCCATCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑cccccc                                                                                                                               <  2:653966/139‑1 (MQ=255)        tgtCCGCCATCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGATCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑ccccccct                                                                                                                             >  2:503413/1‑137 (MQ=255)          tCCGCCATCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑ccccccctga                                                                                                                           >  1:215122/1‑135 (MQ=255)          tCCGCCATCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑ccccccctga                                                                                                                           <  2:708098/139‑5 (MQ=255)          tCCGCCATCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑ccccccctga                                                                                                                           <  1:434288/139‑5 (MQ=255)                 tCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCCTGACGTACaa                                                                                                                     <  2:239305/139‑1 (MQ=255)                         cTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATGCaa                                                                                                             <  1:331392/139‑1 (MQ=255)                               aaCCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATGCAACGCAGt                                                                                                       <  2:339149/139‑1 (MQ=255)                                           ctctGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGtt                                                                                           >  1:723770/1‑139 (MQ=255)                                                   ggCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATc                                                                                   >  2:523128/2‑139 (MQ=255)                                                      gTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCCTGACGTACaa                                                                                                                     >  1:385272/1‑102 (MQ=255)                                                      gTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCCTGACGTACaa                                                                                                                     <  2:385272/102‑1 (MQ=255)                                                      gTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATcaca                                                                                <  1:234695/139‑1 (MQ=255)                                                      gTAGAGGTCAGAACTGAATCCGAACTGCATCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATcaca                                                                                <  2:657318/139‑1 (MQ=255)                                                        agagGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATca                                                                                  >  1:399324/1‑135 (MQ=255)                                                        agagGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATca                                                                                  <  2:399324/135‑1 (MQ=255)                                                        agagGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAgg                                                                              <  2:747393/139‑1 (MQ=255)                                                        agagGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑ccccccc                                                                                                                              <  2:837710/90‑2 (MQ=255)                                                        agagGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑ccccccc                                                                                                                              >  1:837710/1‑89 (MQ=255)                                                          agGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCcgcg                                                                                                                                      >  2:78824/1‑81 (MQ=255)                                                          agGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCcgcg                                                                                                                                      <  1:78824/81‑1 (MQ=255)                                                          agGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATGc                                                                                                               >  1:667297/1‑104 (MQ=255)                                                          agGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATGc                                                                                                               <  2:667297/104‑1 (MQ=255)                                                          agGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCgg                                                                                       >  2:704275/1‑128 (MQ=255)                                                          agGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCgg                                                                                       <  1:704275/128‑1 (MQ=255)                                                           ggTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGGGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGt                                                                           <  2:215122/139‑1 (MQ=255)                                                                      aaTCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGt                                                                                            <  2:186727/111‑1 (MQ=255)                                                                      aaTCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGt                                                                                            >  1:186727/1‑111 (MQ=255)                                                                           tAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCCTGACg                                                                                                                          >  1:377820/1‑76 (MQ=255)                                                                           tAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCCTGACg                                                                                                                          <  2:377820/76‑1 (MQ=255)                                                                                gCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGt                                                                                            <  1:171833/101‑1 (MQ=255)                                                                                gCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGt                                                                                            >  2:171833/1‑101 (MQ=255)                                                                                gCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGAt                                                      >  1:366618/1‑139 (MQ=255)                                                                                   tCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑cccccc                                                                                                                               >  1:579430/1‑62 (MQ=255)                                                                                   tCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑cccccc                                                                                                                               <  2:579430/62‑1 (MQ=255)                                                                                         ccGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCCTGACGTACa                                                                                                                      >  1:609875/1‑66 (MQ=255)                                                                                         ccGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCCTGACGTACa                                                                                                                      <  2:609875/66‑1 (MQ=255)                                                                                         ccGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCt                                             >  1:393210/1‑139 (MQ=255)                                                                                            cgAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTggg                                          >  1:295448/1‑139 (MQ=255)                                                                                            cgAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTggg                                          <  2:723770/139‑1 (MQ=255)                                                                                                         ttctTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCgg                                                                                       >  2:422624/1‑81 (MQ=255)                                                                                                         ttctTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCgg                                                                                       <  1:422624/81‑1 (MQ=255)                                                                                                            ttttAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGACTTTTCTACTGGGCCAGTTTCGGCGTGGt                          >  2:435768/1‑139 (MQ=255)                                                                                                                   gtgCGCTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGc                                                                                         >  1:141695/1‑69 (MQ=255)                                                                                                                   gtgCGCTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGc                                                                                         <  2:141695/69‑1 (MQ=255)                                                                                                                      cgcTGCGGATTATCTTCCGCGCCCCCCCTGACGTAc                                                                                                                       <  2:271634/36‑1 (MQ=255)                                                                                                                      cgcTGCGGATTATCTTCCGCGCCCCCCCTGACGTAc                                                                                                                       >  1:271634/1‑36 (MQ=255)                                                                                                                      cgcTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCGTg                            <  1:312578/127‑1 (MQ=255)                                                                                                                      cgcTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCGTg                            <  1:774224/127‑1 (MQ=255)                                                                                                                      cgcTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCGTg                            >  2:774224/1‑127 (MQ=255)                                                                                                                      cgcTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCGTg                            >  2:312578/1‑127 (MQ=255)                                                                                                                          gCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGGGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCGTGGTATTACGCTCATAGc            <  2:700474/139‑1 (MQ=255)                                                                                                                                tATCTTCCGCGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGa                                                                             <  1:96256/68‑1 (MQ=255)                                                                                                                                tATCTTCCGCGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGa                                                                             >  2:96256/1‑68 (MQ=255)                                                                                                                                    ttCCGCGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCGTGGTATTACGCTCATAGCCGAAAATAcc  <  1:732344/139‑1 (MQ=255)                                                                                                                                     tCCGCGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTg                                                                                          >  1:477014/1‑50 (MQ=255)                                                                                                                                     tCCGCGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTg                                                                                          <  2:477014/50‑1 (MQ=255)                                                                                                                                           |                                                                                                                                    GCAATGTTGTCCGCCATCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCGTGGTATTACGCTCATAGCCGAAAATACC  >  NC_000913/3709418‑3709686

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑