| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,897,050 | +G | coding (277/666 nt) | yieH → | phosphoenolpyruvate and 6‑phosphogluconate phosphatase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,897,046 | 1 | . | G | 92.1% | 122.7 / 3.5 | 38 | coding (273/666 nt) | yieH | phosphoenolpyruvate and 6‑phosphogluconate phosphatase |
| Reads supporting (aligned to +/- strand): ref base . (1/2); new base G (14/21); total (15/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TTTCAAAGGTGTAAAACTGTACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAA‑GGG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTGCACTACTT > NC_000913/3896908‑3897156 | tttCAAAGGTGTAAAACTGTACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGaa < 2:108319/139‑1 (MQ=255) gtAAAACTGTACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAA‑GGG‑GCTGGAg > 2:67544/1‑139 (MQ=255) gTACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGG‑GCTGGAGCGCTCCt > 1:120142/1‑139 (MQ=255) aCGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGG‑GCTGGAGCGCTCCTGt < 2:120142/139‑1 (MQ=255) tATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGG‑GCTGGAGCGCTCCTGTCAGCGAt < 2:830684/139‑1 (MQ=255) tCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGG‑GCTGGAGCGCTCCTGTCAGCGATCa > 2:756651/1‑139 (MQ=255) ttGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAAtgtgtg < 2:608856/139‑1 (MQ=255) tggAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTCCGATTCAGAACGGGAAGCCATCGAAGGGG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAAtgtgtg < 2:631850/137‑1 (MQ=255) aCATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGt < 2:89027/139‑1 (MQ=255) ggTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGG‑GCTGGAGCGCTcc > 1:759581/1‑104 (MQ=255) ggTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGG‑GCTGGAGCGCTcc < 2:759581/104‑1 (MQ=255) tACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACgg > 2:437242/1‑139 (MQ=255) ttAGCGAAAACAGAAGCTGAACACGTTTACCGTGCATAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCTTCGAA‑GGGCGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTATCGGCCCa < 2:392285/139‑1 (MQ=255) gCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGG‑GCTGGAGCGCTCCTGTCAGCg < 2:550305/100‑1 (MQ=255) gCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGG‑GCTGGAGCGCTCCTGTCAGCg > 1:550305/1‑100 (MQ=255) aaCAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACaa > 1:474690/1‑139 (MQ=255) gAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATg < 1:756651/139‑1 (MQ=255) cTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATgcagc < 1:230904/139‑1 (MQ=255) aaCACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAA‑GGG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTc < 1:67544/139‑1 (MQ=255) tACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGc < 2:429666/139‑1 (MQ=255) tACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGc < 2:689407/139‑1 (MQ=255) tACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGc > 1:689407/1‑139 (MQ=255) tGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATGACAAAATGCAGCATTCTATGGGc > 2:723052/1‑134 (MQ=255) tGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATGACAAAATGCAGCATTCTATGGGc < 1:723052/134‑1 (MQ=255) cAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATTTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGa < 2:530602/139‑1 (MQ=255) aaGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCa > 2:725236/1‑78 (MQ=255) aaGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCa < 1:725236/78‑1 (MQ=255) gCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGG‑GCTGGAgcg > 1:538920/1‑49 (MQ=255) gCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGG‑GCTGGAgcg < 2:538920/49‑1 (MQ=255) cTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACa < 1:117875/103‑1 (MQ=255) cTCGGCTGTTCGATTCAGAACTGCAAGCCATCGAAGGGG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACa > 2:117875/1‑103 (MQ=255) gTTCGATTCAGAACTGGAAGCCATCGAAGGGG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTGCACTACtt < 2:474690/139‑1 (MQ=255) gTTCGATTCAGAACTGGAAGCCATCGAAGGGG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTGCACTACtt < 2:300453/139‑1 (MQ=255) aaCTGGAAGCCATCGAAGGGG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCa > 1:396006/1‑54 (MQ=255) aaCTGGAAGCCATCGAAGGGG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCa < 2:396006/54‑1 (MQ=255) aaCTGGAAGCCATCGAAGGGG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAAtgtgtg > 1:511367/1‑61 (MQ=255) aaCTGGAAGCCATCGAAGGGG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAAtgtgtg < 2:511367/61‑1 (MQ=255) gAAGCCATCGAAGGGG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAAtgt > 2:229901/1‑53 (MQ=255) gAAGCCATCGAAGGGG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAAtgt < 1:229901/53‑1 (MQ=255) | TTTCAAAGGTGTAAAACTGTACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAA‑GGG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTGCACTACTT > NC_000913/3896908‑3897156 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |