Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,462,897 (T)7→8 intergenic (‑237/+157) nrdD ← / ← treC anaerobic ribonucleoside‑triphosphate reductase/trehalose‑6‑P hydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,462,8901.T100.0% 113.2 / NA 36intergenic (‑230/+164)nrdD/treCanaerobic ribonucleoside‑triphosphate reductase/trehalose‑6‑P hydrolase
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base T (18/18);  total (18/18)

TTTCGTGGGGAGCTGTAGTAGCAATTATAGTCGATTAATACAACATATTGGGTTGGGACGCATTTTAAAGTCTATATATAGTGCTTTGCATCAAGGATGTTTGAGCTTTTTTTGATGTAGCTCAAAGTAAAAAGCAGAGCGTACGGATGACGGGCGCTACAGCGATATGTAAATTTTTTAATGAATTTGCTGGTTGAAAAATCAACAAAAACAACATACTGACAGACA  >  NC_000913/4462785‑4463012
                                                                                                          |                                                                                                                          
tttCGTGGGGAGCTGTAGTAGCAATTATAGTCGATTAATACAACATATTGGGTTGGGACGCATTTTAAAGTCTATATATAGTGCTTTGCATCAAGGATGTTTGAGCTTTTTTTTGATGTAGCTCAAAGTAAAAAGCaga                                                                                            <  2:155217/139‑1 (MQ=255)
       gggAGCTGTAGTAGCAATTATAGTCGATTAATACAACATATTGGGTTGGGACGCATTTTAAAGTCTATATATAGTGCTTTGCATCAAGGATGTTTGAGCTTTTTTTTGATGTAGCTCAAAGTAAAAAGCAGAGCGTACg                                                                                     >  2:637025/1‑139 (MQ=255)
           gCTGTAGTAGCAATTGTAGTCGATTAATACAACAAATTGGGTTGGGACGCATTTTAAAGTCTATATATAGTGCTTTGCATCAAGGATGTTTGAGCTTTTTTTTGATGTAGCTCAAAGTAAAAAGCAGAGCGTACGGATg                                                                                 >  2:225917/1‑139 (MQ=255)
                agtagCAATTATAGTCGATTAATACAACATATTGGGTTGGGACGCATTTTAAAGTCTATATATAGTGCTTTGCATCAAGGATGTTTGAGCTTTTTTTTGATGTAGCTCAAAGTAAAAAGCAGAGCGTACGGATGACggg                                                                            <  2:137502/139‑1 (MQ=255)
                 gtagCAATTATAGTCGATTAATACAACATATTGGGTTGGGACGCATTTTAAAGTCTATATATAGTGCTTTGCATCAAGGATGTTTGAGCTTTTTTTTGATGTAGCTCAAAGTAAAAAGCAGAGCGTACGGATGACGGgc                                                                           <  1:664008/139‑1 (MQ=255)
                  tagCAATTATAGTCGATTAATACAACATATTGGGTTGGGACGCATTTTAAAGTCTATATATAGTGCTTTGCATCAAGGATGTTTGAGCTTTTTTTTGATGTAGCTCAAAGTAAAAAGCAGAGCGTACGGATGACGGgcg                                                                          <  1:637025/139‑1 (MQ=255)
                                                tgggttgggACGCATTTTAAAGTCTATATATAGTGCTTTGCATCAAGGATGTTTGAGCTTTTTTTTGATGTAGCTCAAAGTAAAAAGCAGAGCGTACGGATGACGGgcg                                                                          <  1:449159/109‑1 (MQ=255)
                                                tgggttgggACGCATTTTAAAGTCTATATATAGTGCTTTGCATCAAGGATGTTTGAGCTTTTTTTTGATGTAGCTCAAAGTAAAAAGCAGAGCGTACGGATGACGGgcg                                                                          >  2:449159/1‑109 (MQ=255)
                                                tgggttgggACGCATTTTAAAGTCTATATATAGTGCTTTGCATCAAGGATGTTTGAGCTTTTTTTTGATGTAGCTCAAAGTAAAAAGCAGAGCGTACGGATGACGGGCGCTACAGCGATATGTAAATTTTTTAATGAAt                                            >  1:477146/1‑139 (MQ=255)
                                                   gttgggACGCATTTTAAAGTCTATATATAGTGCTTTGCATCAAGGATGTTTGAGCTTTTTTTTGATGTAGCTCAAAGTAAAAAGCAGAGCGTACGGATGACGGGCGCTACAGCGATATGTAAATTTTTTAATGAATTTg                                         >  1:447256/1‑139 (MQ=255)
                                                     tgggACGCATTTTAAAGTCTATATATAGTGCTTTGCATCAAGGATGTTTGAGCTTTTTTTTGATGTAGCTCAAAGTAAAAAGCAGAGCGTACGGATGACggg                                                                            >  1:37283/1‑102 (MQ=255)
                                                     tgggACGCATTTTAAAGTCTATATATAGTGCTTTGCATCAAGGATGTTTGAGCTTTTTTTTGATGTAGCTCAAAGTAAAAAGCAGAGCGTACGGATGACggg                                                                            <  2:37283/102‑1 (MQ=255)
                                                       ggACGCATTTTAAAGTCTATATATAGTGCATTGCATCAAGGATGTTTGAGCTTTTTTTTGATGTAGCTCAAAGTCAAAAGCAGAGCGTACGGATGACGGGCGCTACAGCGATATGTAAATTTTTTAATGAATTTGCTgg                                     >  1:560664/1‑139 (MQ=255)
                                                            cATTTTAAAGTCTATATATAGTGCTTTGCATCAAGGATGTTTGAGCTTTTTTTTGATGTAGCTCAAAGTAAAAAGCAGAGCGTACGGATGACGGGCGCTACAGCGATATGTAAATTTTTTAATGAATTTGCTGGTTGaa                                >  2:477324/1‑139 (MQ=255)
                                                                    aGTCTATATATAGTGCTTTGCATCAAGGATGTTTGAGCTTTTTTTTGATGTAGCTCAAAGTAAAAAGCAGAGCGTACGGATGACGGGCGCTACAGCGATATGTAAATTTTTTAATGAATTTGCTGGTTGAAAAATcaac                        <  1:477324/139‑1 (MQ=255)
                                                                      tCTATATATAGTGCTTTGCATCAAGGATGTTTGAGCTTTTTTTTGATGTAGCTCAAAGTAAAAAGCAGAGCGTACGGATGACGGGCGCTACAGCGATATGTAAATTTTTTAATGAATTTGCTGGTTGAAAAATcaacaa                      <  2:161554/139‑1 (MQ=255)
                                                                               aGTGCTTTGCATCAAGGATGTTTGAGCTTTTTTTTGATGTAGCTCAAAGTAAAAAGCAGAGCGTa                                                                                       <  2:465760/65‑1 (MQ=255)
                                                                               aGTGCTTTGCATCAAGGATGTTTGAGCTTTTTTTTGATGTAGCTCAAAGTAAAAAGCAGAGCGTa                                                                                       >  1:465760/1‑65 (MQ=255)
                                                                                gTGCTTTGCATCAAGGATGTTTGAGCTTTTTTTTGATGTAGCTCAAAGTAAAAAGCAGAGCGTa                                                                                       <  2:325575/64‑1 (MQ=255)
                                                                                gTGCTTTGCATCAAGGATGTTTGAGCTTTTTTTTGATGTAGCTCAAAGTAAAAAGCAGAGCGTa                                                                                       >  1:325575/1‑64 (MQ=255)
                                                                                gTGCTTTGCATCAAGGATGTTTGAGCTTTTTTTTGATGTAGCTCAAAGTAAAAAGCAGAGCGTACGGATGACggg                                                                            <  2:281445/75‑1 (MQ=255)
                                                                                gTGCTTTGCATCAAGGATGTTTGAGCTTTTTTTTGATGTAGCTCAAAGTAAAAAGCAGAGCGTACGGATGACggg                                                                            >  1:281445/1‑75 (MQ=255)
                                                                                gTGCTTTGCATCAAGGATGTTTGAGCTTTTTTTTGATGTAGCTCAAAGTAAAAAGCAGAGCGTACGGATGACGGGCGCTACAGCg                                                                  <  2:836749/85‑1 (MQ=255)
                                                                                gTGCTTTGCATCAAGGATGTTTGAGCTTTTTTTTGATGTAGCTCAAAGTAAAAAGCAGAGCGTACGGATGACGGGCGCTACAGCg                                                                  >  1:836749/1‑85 (MQ=255)
                                                                                 tGCTTTGCATCAAGGATGTTTGAGCTTTTTTTTGATGTAGCTCAAAGTAAAAAGCa                                                                                              <  1:496352/56‑1 (MQ=255)
                                                                                 tGCTTTGCATCAAGGATGTTTGAGCTTTTTTTTGATGTAGCTCAAAGTAAAAAGCa                                                                                              >  2:496352/1‑56 (MQ=255)
                                                                                  gCTTTGCATCAAGGATGTTTGAGCTTTTTTTTGATGTAGCTCAAAGTAAAAAGCAGAGCg                                                                                         >  1:81162/1‑60 (MQ=255)
                                                                                  gCTTTGCATCAAGGATGTTTGAGCTTTTTTTTGATGTAGCTCAAAGTAAAAAGCAGAGCg                                                                                         <  2:81162/60‑1 (MQ=255)
                                                                                  gCTTTGCATCAAGGATGTTTGAGCTTTTTTTTGATGTAGCTCAAAGTAAAAAGCAGAGCGTACGGATGACGGGCGCTACAGc                                                                   >  1:615025/1‑82 (MQ=255)
                                                                                  gCTTTGCATCAAGGATGTTTGAGCTTTTTTTTGATGTAGCTCAAAGTAAAAAGCAGAGCGTACGGATGACGGGCGCTACAGc                                                                   <  2:615025/82‑1 (MQ=255)
                                                                                  gCTTTGCATCAAGGATGTTTGAGCTTTTTTTTGATGTAGCTCAAAGTAAAAAGCAGAGCGTACGGATGACGGGCGCTACAGCGATATGTAAATTTTTTAATGAATTTGCTGGTTGAAAAATCAACAAAAACAACATACt          >  2:477476/1‑139 (MQ=255)
                                                                                    tttGCATCAAGGATGTTTGAGCTTTTTTTTGATGTAGCTCAAAGTAAAAAGCa                                                                                              >  2:305603/1‑53 (MQ=255)
                                                                                    tttGCATCAAGGATGTTTGAGCTTTTTTTTGATGTAGCTCAAAGTAAAAAGCa                                                                                              <  1:305603/53‑1 (MQ=255)
                                                                                      tGCATCAAGGATGTTTGAGCTTTTTTTTGATGTAGCTCAAAGTAAAAAGCAGAGCGTAc                                                                                      >  2:328134/1‑59 (MQ=255)
                                                                                      tGCATCAAGGATGTTTGAGCTTTTTTTTGATGTAGCTCAAAGTAAAAAGCAGAGCGTAc                                                                                      <  1:328134/59‑1 (MQ=255)
                                                                                          tCAAGGATGTTTGAGCTTTTTTTTGATGTAGCTCAAAGTAAAAAGCAGAGCGTACGGATGACGGGCGCTACAGCGATATGTAAATTTTTTAATGAATTTGCTGGTTGAAAAATCAACAAAAACAACATACTgacagaca  <  1:477476/139‑1 (MQ=255)
                                                                                                          |                                                                                                                          
TTTCGTGGGGAGCTGTAGTAGCAATTATAGTCGATTAATACAACATATTGGGTTGGGACGCATTTTAAAGTCTATATATAGTGCTTTGCATCAAGGATGTTTGAGCTTTTTTTGATGTAGCTCAAAGTAAAAAGCAGAGCGTACGGATGACGGGCGCTACAGCGATATGTAAATTTTTTAATGAATTTGCTGGTTGAAAAATCAACAAAAACAACATACTGACAGACA  >  NC_000913/4462785‑4463012

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: