Predicted mutation
evidence seq id position mutation annotation gene description
MC JC NC_000913 1,265,001 1086 bp→GAGG [prfA] [prfA]

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 1265001 1266086 1086 23 [0] [0] 20 [prfA] [prfA]

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 12650000 (0.000)20 (0.620)
+GAGG
17/198 0.7 100% intergenic (+30/‑12) hemA/prfA glutamyl tRNA reductase/peptide chain release factor RF‑1
?NC_000913 1266087 = 0 (0.000)coding (1076/1083 nt) prfA peptide chain release factor RF‑1

TGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/1264864‑1265000
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑AGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAGCCAACTTCAGGCGAGCGAAAGCCCGCGGCGTGATGCTGAAATCCTGCTGGAGCATGTTACCGGCAGAGG  >  NC_000913/1266087‑1266197
                                                                                                                                         ||||                                                                                                               
TGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGGA                                                                                                                   >  1:219209/1‑139
                  ATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGGAGGAGGAATAATGGAATAT                                                                                                 >  1:464827/1‑139
                          ACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGGAGGAGGAATAATGGAATATCAACACTG                                                                                         >  2:202848/1‑139
                                                        GGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGGAGGAGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAGCCAACTTCAGGC                                                           <  1:202848/139‑1
                                                                CGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGGAGG                                                                                                                 >  1:255142/1‑77
                                                                CGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGGAGG                                                                                                                 <  2:255142/77‑1
                                                                    CGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGGAGGAGGAATAA                                                                                                         >  2:636727/1‑81
                                                                    CGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGGAGGAGGAATAA                                                                                                         <  1:636727/81‑1
                                                                               TCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGGAGGAGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAGCCAACTTCAGGC                                                           >  2:97187/1‑116
                                                                               TCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGGAGGAGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAGCCAACTTCAGGC                                                           <  1:97187/116‑1
                                                                                   CGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGGAGGAGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAGCCAACTTCAGGCGAGCGAAAGCCCGCGGCGTGATGCTGA                                >  1:425053/1‑139
                                                                                   CGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGGAGGAGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAGCCAACTTCAGGCGAGCGAAAGCCCGCGGCGTGATGCTGA                                >  2:521330/1‑139
                                                                                    GCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGGAGGAGGAATAATGGAATA                                                                                                  <  2:356841/72‑1
                                                                                    GCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGGAGGAGGAATAATGGAATA                                                                                                  >  1:356841/1‑72
                                                                                         AGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGGAGGAGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAGCCAACTTCAGGCGAGCGAAAGCCCGCGGCGTGATGCTGAAATCCT                          <  2:425053/139‑1
                                                                                                     GAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGGAGGAGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAGCCAACTTCAGGCGAGCGAAAGCCCGCGGCGTGATGCTGAAATCCTGCTGGAGCATGT              <  1:521330/139‑1
                                                                                                     GAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGGAGGAGGAATAATGGAATATC                                                                                                <  1:502547/57‑1
                                                                                                     GAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGGAGGAGGAATAATGGAATATC                                                                                                >  2:502547/1‑57
                                                                                                                CATCATTTTCTTTTTTTACAGGGTGGAGGAGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAGCCAACTTCAGGCGAGCGAAAGCCCGCGGCGTGATGCTGAAATCCTGCTGGAGCATGTTACCGGCAGAG   >  2:119876/1‑139
                                                                                                                CATCATTTTCTTTTTTTACAGGGTGGAGGAGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAGCCAACTTCAGGCGAGCGAAAGCCCGCGGCGTGATGCTGAAATCCTGCTGGAGCATGTTACCGGCAGAG   >  2:86601/1‑139
                                                                                                                 ATCATTTTCTTTTTTTACAGGGTGGAGGAGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAGCCAACTTCAGGCGAGCGAAAGCCCGCGGCGTGATGCTGAAATCCTGCTGGAGCATGTTACCGGCAGAGG  <  1:164034/139‑1
                                                                                                                   CATTTTCTTTTTTTACAGGGTGGAGGAGGAATAATGGAATATCAACACTGGTTACGTGA                                                                                >  1:399685/1‑59
                                                                                                                   CATTTTCTTTTTTTACAGGGTGGAGGAGGAATAATGGAATATCAACACTGGTTACGTGA                                                                                <  2:399685/59‑1
                                                                                                                                         ||||                                                                                                               
TGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/1264864‑1265000
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑AGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAGCCAACTTCAGGCGAGCGAAAGCCCGCGGCGTGATGCTGAAATCCTGCTGGAGCATGTTACCGGCAGAGG  >  NC_000913/1266087‑1266197

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.