Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,628,7750GA46.7% 13.6 / 52.4 45A255V (GCG→GTG) rbbAribosome‑associated ATPase: ATP‑binding protein/ATP‑binding membrane protein
Reads supporting (aligned to +/- strand):  ref base G (11/13);  new base A (10/11);  total (21/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.51e-01
Rejected as consensus: Frequency below/above cutoff threshold.

GTGGAATGCGGAAATTAACGTGATCAACGGCAACGAAGGAACCAAAACGCATGGTCAGATCGCGCGCTTCGATGGCAATCTCTGCGTTTTCAGGTTGATACGGTGGGATCACTACCGCCTGATGCGCCTGGCGTTGCGCTTGCGGTAACAGATTTATAAATGCTTCTTCCAGCGTAGCGCTTTGCGTTTGCTGCCGTAGCTCTTCGGCGCTGCCAGTTGCCAGCACTTCTCCGGCATTCATCGCTACCAGCCAGTCGAAGCGTTCGGCCT  >  NC_000913/3628638‑3628907
                                                                                                                                         |                                                                                                                                    
gTGGAATGCGGAAATTAACGTGATCAACGGCAACGAAGGAACCAAAACGCATGGTCAGATCGCGCGCTTCGATGGCAATCTCTGCGTTTTCAGGTTGATACGGTGGGATCACTACCGCCTGATGCGCCTGGCGTTgcgc                                                                                                                                     >  1:291890/1‑139 (MQ=255)
       gCGGAAATTAACGTGATCAACGGCAACGAAGGAACCAAAACGCATGGTCAGATCGCGCGCTTCGATGGCAATCTCTGCGTTTTCAGGTTGATACGGTGGGATCACTACCGCCTGATGCGCCTGGCGTTGCGCTTGCGGt                                                                                                                              <  1:637409/139‑1 (MQ=255)
       gCGGAAATTAACGTGATCAACGGCAACGAAGGAACAAAAACGCATGGTCAGATCGCGCGCTTCGATGGCAATCTCTGCGTTTTCAGGTTGATACGGTGGGATCACTACCGCCTGATGCGCCTGGCGTTGCACTTGCGGt                                                                                                                              <  1:506457/139‑1 (MQ=255)
             aTTAACGTGATCAACGGCAACGAAGGAACCAAAACGCATGGTCAGATCGCGCGCTTCGATGGCAATCTCTGCGTTTTCAGGTTGATACGGTGGGATCACTACCGCCTGATGCGCCTGGCGTTGCACTTGCGGTAACAGa                                                                                                                        <  2:223665/139‑1 (MQ=255)
                   gTGATCAACGGCAACGAAGGAACCAAAACGCATGGTCAGATCGCGCGCTTCGATGGCAATCTCTGCGTTTTCAGGTTGATACGGTGGGATCACTACCGCCTGATGCGCCTGGCGTTGCGCTTGCGGTAACAGATTtata                                                                                                                  <  1:546225/139‑1 (MQ=255)
                    tGATCAACGGCAACGAAGGAACCAAAACGCATGGTCAGATCGCGCGCTTCGATGGCAATCTCTGCGTTTTCAGGTTGATACGGTGGGATCACTACCGCCTGATGCGCCTGGCGTTGCGCTTGCGGTAACAGATTTATaa                                                                                                                 <  2:291890/139‑1 (MQ=255)
                     gATCAACGGCAACGAAGGAACCAAAACGCATGGTCAGATCGCGCGCTTCGATGGCAATCTCTGCGTTTTCAGGTTGATACGGTGGGATCACTACCGCCTGATGCGCCTGGCGTTGCGCTTGCGGTaa                                                                                                                            >  2:708870/1‑127 (MQ=255)
                     gATCAACGGCAACGAAGGAACCAAAACGCATGGTCAGATCGCGCGCTTCGATGGCAATCTCTGCGTTTTCAGGTTGATACGGTGGGATCACTACCGCCTGATGCGCCTGGCGTTGCGCTTGCGGTaa                                                                                                                            <  1:708870/127‑1 (MQ=255)
                                aCGAAGGAACCAAAACGCATGGTCAGATCGCGCGCTTCGATGGCAATCTCTGCGTTTTCAGGTTGATACGGTGGGATCACTACCGCCTGATGCGCCTGGCGTTGCACTTGCGGTaa                                                                                                                            <  1:344520/116‑1 (MQ=255)
                                aCGAAGGAACCAAAACGCATGGTCAGATCGCGCGCTTCGATGGCAATCTCTGCGTTTTCAGGTTGATACGGTGGGATCACTACCGCCTGATGCGCCTGGCGTTGCACTTGCGGTaa                                                                                                                            >  2:344520/1‑116 (MQ=255)
                                             aaCGCATGGTCAGATCGCGCGCTTCGATGGCAATCTCTGCGTTTTCAGGTTGATACGGTGGGATCACTACCGCCTGATGCGCCTGGCGTTGCACTTGCGGTAACAGATTTATaa                                                                                                                 <  2:128057/114‑1 (MQ=255)
                                             aaCGCATGGTCAGATCGCGCGCTTCGATGGCAATCTCTGCGTTTTCAGGTTGATACGGTGGGATCACTACCGCCTGATGCGCCTGGCGTTGCACTTGCGGTAACAGATTTATaa                                                                                                                 >  1:128057/1‑114 (MQ=255)
                                                   tGGTCAGATCGCGCGCTTCGATGGCAATCTCTGCGTTTTCAGGTTGATACGGTGGGATCACTACCGCCTGATGTGCCTGGCGTTGCACTTGCGGTAACAGATTTATAAATGCTTCTTCCAGCGTAGCGCTTTGCGTTtg                                                                                  >  1:218438/1‑139 (MQ=255)
                                                          aTCGCGCGCTTCGATGGCAATCTCTGCGTTTTCAGGTTGATACGGTGGGATCACTACCGCCTGATGCGCCTGGCGTTGCGCTTGCGGTAACAGATTTATAAATGCTTCTTCCAGCGTAGCGCTTTGCGTTtgc                                                                                 <  2:123533/133‑1 (MQ=255)
                                                          aTCGCGCGCTTCGATGGCAATCTCTGCGTTTTCAGGTTGATACGGTGGGATCACTACCGCCTGATGCGCCTGGCGTTGCGCTTGCGGTAACAGATTTATAAATGCTTCTTCCAGCGTAGCGCTTTGCGTTtgc                                                                                 >  1:123533/1‑133 (MQ=255)
                                                              cgcgcTTCGATGGCAATCTCTGCGTTTTCAGGTTGATACGGTGGGATCACTACCGCCTGATGCGCCTGGCGTTGCGCTTGCGGTAAc                                                                                                                           <  1:378395/87‑1 (MQ=255)
                                                              cgcgcTTCGATGGCAATCTCTGCGTTTTCAGGTTGATACGGTGGGATCACTACCGCCTGATGCGCCTGGCGTTGCGCTTGCGGTAAc                                                                                                                           >  2:378395/1‑87 (MQ=255)
                                                              cgcgcTTCGATGGCAATCTCTGCGTTTTCAGGTTGATACGGTGGGATCACTACCGCCTGATGCGCCTGGCGTTGCGCTTGCGGTAACAGATTTATAAATGct                                                                                                            >  2:821373/1‑102 (MQ=255)
                                                              cgcgcTTCGATGGCAATCTCTGCGTTTTCAGGTTGATACGGTGGGATCACTACCGCCTGATGCGCCTGGCGTTGCGCTTGCGGTAACAGATTTATAAATGct                                                                                                            <  1:821373/102‑1 (MQ=255)
                                                              cgcgcTTCGACGGCAATCTCTGCGTTTTCAGGTTGATACGGTGGGATCACTACCGCCTGATGCGCCTGGCGTTGCGCTTGCGGTAACAGATTTATAAATGCttcttc                                                                                                       >  1:535661/1‑107 (MQ=255)
                                                              cgcgcTTCGACGGCAATCTCTGCGTTTTCAGGTTGATACGGTGGGATCACTACCGCCTGATGCGCCTGGCGTTGCGCTTGCGGTAACAGATTTATAAATGCttcttc                                                                                                       <  2:535661/107‑1 (MQ=255)
                                                                             aTCTCTGCGTTTTCAGGTTGATACGGTGGGATCACTACCGCCTGATGCGCCTGGCGTTGCGCTTGCGGTAACAGATTTATAAATGCTTCTTCCAGCGTAGCGCTTTGCGTTTGCTGCCGTAGCTCTTCGGCGCTGCCAg                                                        >  1:739358/1‑139 (MQ=255)
                                                                               ctctGCGTTTTCAGGTTGATACGGTGGGATCACTACCGCCTGATGTGCCTGGCGTTGCACTTGCGGTAACAGATTTATAAATGCTTCTTCCAGCGTAGCGCTTTGCGTTTGCTGCCGTAGCTCTTCGGCGCTGCCAGtt                                                      <  2:218438/139‑1 (MQ=255)
                                                                                        ttCAGGTTGATACGGTGGGATCACTACCGCCTGATGCGCCTGGCGTTGCGCTTGCGGTAACAGATTTATAAATGct                                                                                                            <  1:659757/76‑1 (MQ=255)
                                                                                        ttCAGGTTGATACGGTGGGATCACTACCGCCTGATGCGCCTGGCGTTGCGCTTGCGGTAACAGATTTATAAATGct                                                                                                            >  2:659757/1‑76 (MQ=255)
                                                                                        ttCAGGTTGATACGGTGGGATCACTACCGCCTGATGCGCCTGGCGTTGCGCTTGCGGTAACAGATTTATAAATGCTTCTTCCAGCGTAGCGCTTTGCGTTTGCTGCCGTAGCTCTTCGGCGCTGCCAGTTGCCAGCACt                                             >  1:270560/1‑139 (MQ=255)
                                                                                         tCAGGTTGATACGGTGGGATCACTACCGCCTGATGCGCCTGGCGTTGCGCTTGCGGTAACAGATTTATAAATGCTTCTTCCAGCGTAGCGCTTTGCGTTTGCTGCCGTAGCTCTTCGGCGCTGCCAGTTGCCAGCACtt                                            <  2:739358/139‑1 (MQ=255)
                                                                                            ggTTGATACGGTGGGATCACTACCGCCTGATGCGCCTGGCGTTGCGCTTGCGGTAACAGATTTATAAATGct                                                                                                            >  2:295803/1‑72 (MQ=255)
                                                                                            ggTTGATACGGTGGGATCACTACCGCCTGATGCGCCTGGCGTTGCGCTTGCGGTAACAGATTTATAAATGct                                                                                                            <  1:295803/72‑1 (MQ=255)
                                                                                                 aTACGGTGGGATCACTACCGCCTGATGCGCCTGGCGTTGCACTTGCGGTAACAGATTTATaa                                                                                                                 <  1:520464/62‑1 (MQ=255)
                                                                                                 aTACGGTGGGATCACTACCGCCTGATGCGCCTGGCGTTGCACTTGCGGTAACAGATTTATaa                                                                                                                 >  2:520464/1‑62 (MQ=255)
                                                                                                     ggTGGGATCACTACCGCCTGATGCGCCTGGCGTTGCACTTGCGGTAACAGATTTATAAATGCTTCTTCCAGCGTAGCGCTTTGCGTTtg                                                                                  >  1:154825/1‑89 (MQ=255)
                                                                                                     ggTGGGATCACTACCGCCTGATGCGCCTGGCGTTGCACTTGCGGTAACAGATTTATAAATGCTTCTTCCAGCGTAGCGCTTTGCGTTtg                                                                                  <  2:154825/89‑1 (MQ=255)
                                                                                                           aTCACTACCGCCTGATGCGCCTGGCGTTGCACTTGCGGTAACAGATTTATAAATGct                                                                                                            >  2:518509/1‑57 (MQ=255)
                                                                                                           aTCACTACCGCCTGATGCGCCTGGCGTTGCACTTGCGGTAACAGATTTATAAATGct                                                                                                            <  1:518509/57‑1 (MQ=255)
                                                                                                                 aCCGCCTGATGCGCCTGGCGTTGCACTTGCGGTAACAGATTTATAAATGct                                                                                                            >  2:606101/1‑51 (MQ=255)
                                                                                                                 aCCGCCTGATGCGCCTGGCGTTGCACTTGCGGTAACAGATTTATAAATGct                                                                                                            <  1:606101/51‑1 (MQ=255)
                                                                                                                   cGCCTGATGCGCCTGGCGTTGCGCTTGCGGTAACAGATTTATAAATGct                                                                                                            <  2:52186/49‑1 (MQ=255)
                                                                                                                   cGCCTGATGCGCCTGGCGTTGCGCTTGCGGTAACAGATTTATAAATGct                                                                                                            >  1:52186/1‑49 (MQ=255)
                                                                                                                    gCCTGATGCGCCTGGCGTTGCACTTGCGGTAACAGATTTATAAATGCTTCTTCCAGCGt                                                                                                 <  2:251111/59‑1 (MQ=255)
                                                                                                                    gCCTGATGCGCCTGGCGTTGCACTTGCGGTAACAGATTTATAAATGCTTCTTCCAGCGt                                                                                                 >  1:251111/1‑59 (MQ=255)
                                                                                                                     ccTGATGCGCCTGGCGTTGCGCTTGCGGTAACAGATTTATAAATGCTTCTTCCAGCGTAGCGCTTTGCGTTTGCTGCCGTAGCTCTTCGGCGCTGCCAGTTGCCAGCACTTCTCCGGCATTCATCGCTACCAGCCAGTc                >  1:38417/1‑139 (MQ=255)
                                                                                                                     ccTGATGCGCCTGGCGTTGCACTTGCGGTAACAGATTTATAAATGCTTCTTCCAGCGTAGCGCTTTGCGTTTGCTGCCGTAGCTCTTCGgc                                                                <  1:354333/91‑1 (MQ=255)
                                                                                                                     ccTGATGCGCCTGGCGTTGCACTTGCGGTAACAGATTTATAAATGCTTCTTCCAGCGTAGCGCTTTGCGTTTGCTGCCGTAGCTCTTCGgc                                                                >  2:354333/1‑91 (MQ=255)
                                                                                                                                 ggCGTTGCGCTTGCGGTAACAGATTTATAAATGCTTCTTCCAGCGTAGCGCTTTGCGTTTGCTGCCGTAGCTCTTCGGCGCTGCCAGTTGCCAGCACTTCTCCGGCATTCATCGCTACCAGCCAGTCGAAGCGTTCGGc    <  2:270560/139‑1 (MQ=255)
                                                                                                                                   cGTTGCACTTGCGGTAACAGATTTATAAATGCTTCTTCCAGCGTAGCGCTTTGCGTTTGCTGCCGTAGCTCTTCGGCGCTGCCAGTTGCCAGCACTTCTCCGGCATTCATCGCTACCAGCCAGTCGAAGCGTTCGGCct  >  1:782794/1‑139 (MQ=255)
                                                                                                                                         gcTTGCGGTAACAGATTTATAAATGCTTCTTCCAGCGTAGCGCTTTGCGTTTGCTGCCGTAGCTCTTCGGCGCTGCCAGTTGCCAGCACTtct                                          <  2:157125/93‑1 (MQ=255)
                                                                                                                                         gcTTGCGGTAACAGATTTATAAATGCTTCTTCCAGCGTAGCGCTTTGCGTTTGCTGCCGTAGCTCTTCGGCGCTGCCAGTTGCCAGCACTtct                                          >  1:157125/1‑93 (MQ=255)
                                                                                                                                         |                                                                                                                                    
GTGGAATGCGGAAATTAACGTGATCAACGGCAACGAAGGAACCAAAACGCATGGTCAGATCGCGCGCTTCGATGGCAATCTCTGCGTTTTCAGGTTGATACGGTGGGATCACTACCGCCTGATGCGCCTGGCGTTGCGCTTGCGGTAACAGATTTATAAATGCTTCTTCCAGCGTAGCGCTTTGCGTTTGCTGCCGTAGCTCTTCGGCGCTGCCAGTTGCCAGCACTTCTCCGGCATTCATCGCTACCAGCCAGTCGAAGCGTTCGGCCT  >  NC_000913/3628638‑3628907

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑