Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,676,8780GA58.2% 17.5 / 62.9 55P491P (CCC→CCT) yhjGputative inner membrane‑anchored periplasmic AsmA family protein
Reads supporting (aligned to +/- strand):  ref base G (12/11);  new base A (16/16);  total (28/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

ACCAGCCCGTCATTCATCAACAGTTTCAGGTTGCCGTTACTGTTGCCTAAAAGTGCCGCCACCGAGTTACCGCTACCGCGTAGTTCCGCGTCACCGTTCATTTCCCCCAGCGTCTTCTGCATCAGTTCCACATCGGGCATCAGTTCTTTCAGTTTCAGTCGACGAGCCTGAATATCTGCCCGCCCCTGCATCGGCTTTTTATCGCCTTCCAGATGAATATTCGCCGCAATGCTGCCGCCCGCCATGCCAAATTTCAGCGGTTGCAGGCGCAGG  >  NC_000913/3676744‑3677016
                                                                                                                                      |                                                                                                                                          
aCCAGCCCGTCATTCATCAACAGTTTCAGGTTGCCGTTACTGTTGCCTAAAAGTGCCGCCACCGAGTTACCGCTACCGCGTAGTTCCGCGTCACCGTTCATTTCCCCCAGCGTCTTCTGCATCAGTTCCACATCGGGCa                                                                                                                                        >  1:6873/1‑139 (MQ=255)
    gCCCGTCATTCATCAACAGTTTCAGGTTGCCGTTACTGTTGCCTAAAAGTGCCGCCACCGAGTTACCGCTACCGCGTAGTTCCGCGTCACCGTTCATTTCCCCCAGCGTCTTCTGCATCAGTTCCACATCGGGCATCAg                                                                                                                                    <  2:38306/139‑1 (MQ=255)
       cGTCATTCATCAACAGTTTCAGGTTGCCGTTACTGTTGCCTAAAAGTGCCGCCACCGAGTTACCGCTACCGCGTAGTTCCGCGTCACCGTTCATTTCCCCCAGCGTCTTCTGCATCAGTTCCACATCAGGCATCAGTTc                                                                                                                                 >  2:236796/1‑139 (MQ=255)
          cattcatCAACAGTTTCAGGTTGCCGTTACTGTTGCCTAAAAGTGCCGCCACCGAGTTACCGCTACCGCGTAGTTCCGCGTCACCGTTCATTTCCCCCAGCGTCTTCTGCATCAGTTCCACATCGGGCATCAGTTCttt                                                                                                                              <  2:6873/139‑1 (MQ=255)
          cattcatCAACAGTTTCAGGTTGCCGTTACTGTTGCCTAAAAGTGCCGCCACCGAGTTACCGCTACCGCGTAGTTCCGCGTCACCGTTCATTTCCCCCAGCGTCTTCTGCATCAGTTCCACATCAGGCATCAGTTCttt                                                                                                                              >  2:717008/1‑139 (MQ=255)
          cattcatCAACAGTTTCAGGTTGCCGTTACTGTTGCCTAAAAGTGCCGCCACCGAGTTACCGCTACCGCGTAGTTCCGCGTCACCGTTCATTTCCCCCAGCGTCTTCTGCATCAGTTCCACATCAGGCATCAGTTCttt                                                                                                                              >  2:164513/1‑139 (MQ=255)
                  aaCAGTTTCAGGTTGCCGTTACTGTTGCCTAAAAGTGCCGCCACCGAGTTACCGCTACCGCGTAGTTCCGCGTCACCGTTCATTTCCCCCAGCGTCTTCTGCATCAGTTCCACATCAGGCATCAGTTCtttcagtttca                                                                                                                      <  1:164513/139‑1 (MQ=255)
                  aaCAGTTTCAGGTTGCCGTTACTGTTGCCTAAAAGTGCCGCCACCGAGTTACCGCTACCGCGTAGTTCCGCGTCACCGTTCATTTCCCCCAGCGTCTTCTGCATCAGTTCCACATCAGGCATCAGTTCtttcagtttca                                                                                                                      <  1:717008/139‑1 (MQ=255)
                    cAGTTTCAGGTTGCCGTTACTGTTGCCTAAAAGTGCCGCCACCGAGTTACCGCTACCGCGTAGTTCCGCGTCACCGTTCATTTCCCCCAGCGTCTTCTGCATCAGTTCCACATCGGGCATCAGTTCTttcagtttcagt                                                                                                                    >  2:656657/1‑139 (MQ=255)
                       tttCAGGTTGCCGTTACTGTTGCCTAAAAGTGCCGCCACCGAGTTACCGCTACCGCGTAGTTCCGCGTCACCGTTCATTTCCCCCAGCGTCTTCTGCATCAGTTCCACATCAGGCATCAGTTCTTTCAGTTTCAGTcga                                                                                                                 >  1:332395/1‑139 (MQ=255)
                       tttCAGGTTGCCGTTACTGTTGCCTAAAAGTGCCGCCACCGAGTTACCGCTACCGCGTAGTTCCGCGTCACCGTTCATTTCCCCCAGCGTCTTCTGCATCAGTTCCACATCAGGCATCAGTTCTTTCAGTTTCAGTcga                                                                                                                 <  2:332395/139‑1 (MQ=255)
                       tttCAGGTTGCCGTTACTGTTGCCTAAAAGTGCCGCCACCGAGTTACCGCTACCGCGTAGTTCCGCGTCACCGTTCATTTCCCCCAGCGTCTTCTGCATCAGTTCCACATCAGGCATCAGTTCTTTCAGTTTCAGTcga                                                                                                                 <  1:7768/139‑1 (MQ=255)
                        ttCAGGTTGCCGTTACTGTTGCCTAAAAGTGCCGCCACCGAGTTACCGCTACCGCGTAGTTCCGCGTCACCGTTCATTTCCCCCAGCGTCTTCTGCATCAGTTCCACATCAGGCATCAGTTCTTTCAGTTTCAGTcgac                                                                                                                <  2:770794/139‑1 (MQ=255)
                         tCAGGTTGCCGTTACTGTTGCCTAAAAGTGCCGCCACCGAGTTACCGCTACCGCGTAGTTCCGCGTCACCGTTCATTTCCCCCAGCGTCTTCTGCATCAGTTCCACATCGGGCATCAGTTCTTTCAGTTTCAGTcgacg                                                                                                               >  1:572275/1‑139 (MQ=255)
                         tCAGGTTGCCGTTACTGTTGCCTAAAAGTGCCGCCACCGAGTTACCGCTACCGCGTAGTTCCGCGTCACCGTTCATTTCCCCCAGCGTCTTCTGCATCAGTTCCACATCAGGCATCAGTTCTTTCAGTTTCAGTcgacg                                                                                                               <  2:766981/139‑1 (MQ=255)
                               tGCCGTTACTGTTGCCTAAAAGTGCCGCCACCGAGTTACCGCTACCGCGTAGTTCCGCGTCACCGTTCATTTCCCCCAGCGTCTTCTGCATCAGTTCCACATCAGGCATCAGTTCTTTCAGTTTCAGTcgac                                                                                                                >  2:178155/1‑132 (MQ=255)
                               tGCCGTTACTGTTGCCTAAAAGTGCCGCCACCGAGTTACCGCTACCGCGTAGTTCCGCGTCACCGTTCATTTCCCCCAGCGTCTTCTGCATCAGTTCCACATCAGGCATCAGTTCTTTCAGTTTCAGTcgac                                                                                                                <  1:178155/132‑1 (MQ=255)
                                         tttcccTAAAACTGCCGCCACCTAGTTACCGCTACCGCGTAGTTCCGCGTCACCGTTCATTTCCCCCAGCGTCTTCTGCATCAGTCCCACATCGGGCATCCGTTCTTTCAGTTTCAGTCGACGAGCCTGAATATCTgcc                                                                                               <  2:96521/135‑1 (MQ=255)
                                         gTTGCCTAAAAGTGCCGCCACCGAGTTACCGCTACCGCGTAGTTCCGCGTCACCGTTCATTTCCCCCAGCGTCTTCTGCATCAGTTCCACATCGGGCATCAGTTCTTTCAGTTTCAGTCGACGAGCCTGAATATCTgcc                                                                                               <  2:572275/139‑1 (MQ=255)
                                            gCCTAAAAGTGCCGCCACCGAGTTACCGCTACCGCGTAGTTCCGCGTCACCGTTCATTTCCCCCAGCGTCTTCTGCATCAGTTCCACATCAGGCATCAGTTCTTTCAGTTTCAGTCGACGAGCCTGAATATCTgcccgc                                                                                            >  2:578051/1‑139 (MQ=255)
                                                       ccgccACCGAGTTACCGCTACCGCGTAGTTCCGCGTCACCGTTCATTTCCCCCAGCGTCTTCTGCATCAGTTCCACATCAGGCATCAGTTCTTTCAGTTTCAGTCGACGAGCCt                                                                                                          >  1:709272/1‑114 (MQ=255)
                                                       ccgccACCGAGTTACCGCTACCGCGTAGTTCCGCGTCACCGTTCATTTCCCCCAGCGTCTTCTGCATCAGTTCCACATCAGGCATCAGTTCTTTCAGTTTCAGTCGACGAGCCt                                                                                                          <  2:709272/114‑1 (MQ=255)
                                                       ccgccACCGAGTTACCGCTACCGCGTAGTTCCGCGTCACCGTTCATTTCCCCCAGCGTCTTCTGCATCAGTTCCACATCAGGCATCAGTTCTTTCAGTTTCAGTCGACGAGCCTGAATATCTGCCCGCCCCTGCATCgg                                                                                 >  2:486246/1‑139 (MQ=255)
                                                        cgccACCGAGTTACCGCTACCGCGTAGTTCCTCGTCACCGTTCATTTCCCCCAGCGTCTTCTGCATCAGTTCCACATCAGGCATCAGTTCTTTCAGTTTCAGTCGACGAGCCTGAATATCTGCCCGCCCCTGCATCGGc                                                                                <  2:765824/139‑1 (MQ=255)
                                                        cgccACCGAGTTACCGCTACCGCGTAGTTCCGCGTCACCGTTCATTTCCCCCAGCGTCTTCTGCATCAGTTCCACATCAGGCATCAGTTCTTTCAGTTTCAGTCGACGAGCCTGAATATCTGCCCGCCCCTGCATCGGc                                                                                >  1:765824/1‑139 (MQ=255)
                                                               gAGTTACCGCTACCGCGTAGTTCCGCGTCACCGTTCATTTCCCCCAGCGTCTTCTGCATCAGTTCCACATCAGGCATCAGTTCTTTCAGTTTCAGTCGACGAGCCTGAATATCTgcc                                                                                               <  1:769234/117‑1 (MQ=255)
                                                               gAGTTACCGCTACCGCGTAGTTCCGCGTCACCGTTCATTTCCCCCAGCGTCTTCTGCATCAGTTCCACATCAGGCATCAGTTCTTTCAGTTTCAGTCGACGAGCCTGAATATCTgcc                                                                                               >  2:769234/1‑117 (MQ=255)
                                                                   taccgctaccgcGTAGTTCCGCGTCACCGTTCATTTCCCCCAGCGTCTTCTGCATCAGTTCCACATCAGGCATCAGTTCTTTCAGTTTCAGTCGACGAGCCTGAATATCTGCCCGCCCCTGCATCGGCTTTTTATCGcc                                                                     <  1:486246/139‑1 (MQ=255)
                                                                     ccgctaccgcGTAGTTCCGCGTCACCGTTCATTTCCCCCAGCGTCTTCTGCATCAGTTCCACATCAGGCATCAGTTCTTTCAGTTTCAGTCGACGAGCCTGAATATCTGCCCGCCCCTGCATCGGCTTTTTATCGCCtt                                                                   <  1:578051/139‑1 (MQ=255)
                                                                        ctaccgcGTAGTTCCGCGTCACCGTTCATTTCCCCCAGCGTCTTCTGCATCAGTTCCACATCAGGCATCAGTTCTTTCAGTTTCAGTCGACGAGCCTGAatat                                                                                                    <  1:165463/103‑1 (MQ=255)
                                                                        ctaccgcGTAGTTCCGCGTCACCGTTCATTTCCCCCAGCGTCTTCTGCATCAGTTCCACATCAGGCATCAGTTCTTTCAGTTTCAGTCGACGAGCCTGAatat                                                                                                    >  2:165463/1‑103 (MQ=255)
                                                                        ctaccgcGTAGTTCCGCGTCACCGTTCATTTCCCCCAGCGTCTTCTGCATCAGTTCCACATCAGGCATCAGTTCTTTCAGTTTCAGTCGACGAGCCTGAATATCTGCCCGCCCCTGCATCGGCTTTTTATCGCCTTCCa                                                                <  1:236796/139‑1 (MQ=255)
                                                                          accgcGTAGTTCCGCGTCACCGTTCATTTCCCCCAGCGTCTTCTGCATCAGTTCCACATCg                                                                                                                                            <  2:410399/61‑1 (MQ=255)
                                                                          accgcGTAGTTCCGCGTCACCGTTCATTTCCCCCAGCGTCTTCTGCATCAGTTCCACATCg                                                                                                                                            >  1:410399/1‑61 (MQ=255)
                                                                          accgcGTAGTTCCGCGTCACCGTTCATTTCCCCCAGCGTCTTCTGCATCAGTTCCACATCAGGCATCAGTTCTTTCAGTTTCAGTCGACGAGCCTGAATATCTg                                                                                                 <  2:742804/104‑1 (MQ=255)
                                                                          accgcGTAGTTCCGCGTCACCGTTCATTTCCCCCAGCGTCTTCTGCATCAGTTCCACATCAGGCATCAGTTCTTTCAGTTTCAGTCGACGAGCCTGAATATCTg                                                                                                 >  1:742804/1‑104 (MQ=255)
                                                                          accgcGTAGTTCCGCGTCACCGTTCATTTCCCCCAGCGTCTTCTGCATCAGTTCCACATCAGGCATCAGTTCTTTCAGTTTCAGTCGACGAGCCTGAATATCTGCCCGCCCCTGCAt                                                                                    >  2:36726/1‑117 (MQ=255)
                                                                          accgcGTAGTTCCGCGTCACCGTTCATTTCCCCCAGCGTCTTCTGCATCAGTTCCACATCAGGCATCAGTTCTTTCAGTTTCAGTCGACGAGCCTGAATATCTGCCCGCCCCTGCAt                                                                                    <  1:36726/117‑1 (MQ=255)
                                                                            cgcgTAGTTCCGCGTCACCGTTCATTTCCCCCAGCGTCTTCTGCATCAGTTCCACATCAGGCATCAGTTCTTTCAGTTTCAGTCGACGAGCCTGAATATCTGCCCGCCCCTGCATCGGCTTTTTATCGCCTTCCAGATg                                                            >  1:371356/1‑139 (MQ=255)
                                                                              cgTAGTTCCGCGTCACCGTTCATTTCCCCCAGCGTCTTCTGCATCAGTTCCACATCGGGCATCAGTTCTTTCAGTTTCAGTCGACGAGCCTGAATATCTGCCCGCCCCTGCATCGGCTTTTTATCGCCTTCCAGATGaa                                                          >  1:100748/1‑139 (MQ=255)
                                                                                  gTTCCGCGTCACCGTTCATTTCCCCCAGCGTCTTCTGCATCAGTTCCACATCGGGCATCAGTTCTTTCAGTTTCAGTcga                                                                                                                 <  1:475212/80‑1 (MQ=255)
                                                                                  gTTCCGCGTCACCGTTCATTTCCCCCAGCGTCTTCTGCATCAGTTCCACATCGGGCATCAGTTCTTTCAGTTTCAGTcga                                                                                                                 >  2:475212/1‑80 (MQ=255)
                                                                                  gTTCCGCGTCACCGTTCATTTCCCCCAGCGTCTTCTGCATCAGTTCCACATCGGGCATCAGTTCTTTCAGTTTCAGTCGACGAGCCTGAATATCTGCCCGCCCCTGCATCGGCTTTTTATCGCCTTCCAGATGAATAtt                                                      <  1:380389/139‑1 (MQ=255)
                                                                                   ttCCGCGTCACCGTTCATTTCCCCCAGCGTCTTCTGCATCAGTTCCACATCGGGCATCAGTTCTTTCAGTTTCAGTCGACGAGCCTGAATATCTgcc                                                                                               <  2:676833/97‑1 (MQ=255)
                                                                                   ttCCGCGTCACCGTTCATTTCCCCCAGCGTCTTCTGCATCAGTTCCACATCGGGCATCAGTTCTTTCAGTTTCAGTCGACGAGCCTGAATATCTgcc                                                                                               >  1:676833/1‑97 (MQ=255)
                                                                                      cgcgTCACCGTTCATTTCCCCCAGCGTCTTCTGCATCAGTTCCACATCGGGCATCAGTTCTTTCAGTTTCAGTcga                                                                                                                 <  2:408826/76‑1 (MQ=255)
                                                                                      cgcgTCACCGTTCATTTCCCCCAGCGTCTTCTGCATCAGTTCCACATCGGGCATCAGTTCTTTCAGTTTCAGTcga                                                                                                                 >  1:408826/1‑76 (MQ=255)
                                                                                           cACCGTTCATTTCCCCCAGCGTCTTCTGCATCAGTTCCACATCGGGCATCAGTTCTTTCAGTTTCAGTCGACGAGCCTGAATATCTGCCCGCCCCTGCATCGGCTTTTTATCGCCTTCCAGATGAATAtt                                                      <  2:658866/130‑1 (MQ=255)
                                                                                           cACCGTTCATTTCCCCCAGCGTCTTCTGCATCAGTTCCACATCGGGCATCAGTTCTTTCAGTTTCAGTCGACGAGCCTGAATATCTGCCCGCCCCTGCATCGGCTTTTTATCGCCTTCCAGATGAATAtt                                                      >  1:658866/1‑130 (MQ=255)
                                                                                             ccGTTCATTTCCCCCAGCGTCTTCTGCATCAGTTCCACATCGGGCATCAGTTCttt                                                                                                                              >  2:381243/1‑56 (MQ=255)
                                                                                             ccGTTCATTTCCCCCAGCGTCTTCTGCATCAGTTCCACATCGGGCATCAGTTCttt                                                                                                                              <  1:381243/56‑1 (MQ=255)
                                                                                                       cccccAGCGTCTTCTGCATCAGTTCCACATCGGGCATCAGTTCTTTCAGTTTCAGTCGACGAGCCTGAATATCTGCCCGCCCCTGCATCGGCTTTTTATCGCCTTCCAGATGAATATTCGCCGCAATGCTGccgcccgc                                 >  1:695032/1‑139 (MQ=255)
                                                                                                                        aTCAGTTCCACATCGGGCATCAGTTCTTTCAGTTTCAGTCGACGAGCCTGAATATCTGCCCGCCCCTGCATCGGCTTTTTATCg                                                                       >  2:49092/1‑84 (MQ=255)
                                                                                                                        aTCAGTTCCACATCGGGCATCAGTTCTTTCAGTTTCAGTCGACGAGCCTGAATATCTGCCCGCCCCTGCATCGGCTTTTTATCg                                                                       <  1:49092/84‑1 (MQ=255)
                                                                                                                            gTTCCACATCAGGCATCAGTTCTTTCAGTTTCAGTCGACGAGCCTGAATATCTGCCCGCCCCTGCATCGGCTTTTTATCGCCTTCCAGATGAATATTCGCCGCAATGCTGCCGCCCGCCATGCCAAATTACAGCGGTTg            >  2:734042/1‑139 (MQ=255)
                                                                                                                             ttCCACATCAGGCATCAGTTCTTTCAGTTTCAGTCGACGAGCCTGAATATCTGCCCGCCCCTACATCGGCTTTTTATCGCCTTCCAGATGAATATTCGCCGCAATGCTGCCGCCCGCCATGCCAAATTTCAGCGGTTGc           >  2:205754/1‑139 (MQ=255)
                                                                                                                                  caTCGGGCATCAGTTCTTTCAGTTTCAGTCGACGAGCCTGAATATCTGCCCGCCCCTGCATCGGCTTTTTATCGCCTTCCAGATGAATATTCGCCGCAATGCTGCCGCCCGCCATGCCAAATTTCAGCGGTTGCAGgcg      >  2:235692/1‑139 (MQ=255)
                                                                                                                                      gggCATCAGTTCTTTCAGTTTCAGTCGACGAGCCTGAATATCTGCCCGCCCCTGCATCGGCTTTTTATCGCCTTCCAGATGAATATTCGCCGCAATGCTGCCGCCCGCCATGCCAAATTTCAGCGGTTGCAGGCGCAgg  >  2:248688/1‑139 (MQ=255)
                                                                                                                                      |                                                                                                                                          
ACCAGCCCGTCATTCATCAACAGTTTCAGGTTGCCGTTACTGTTGCCTAAAAGTGCCGCCACCGAGTTACCGCTACCGCGTAGTTCCGCGTCACCGTTCATTTCCCCCAGCGTCTTCTGCATCAGTTCCACATCGGGCATCAGTTCTTTCAGTTTCAGTCGACGAGCCTGAATATCTGCCCGCCCCTGCATCGGCTTTTTATCGCCTTCCAGATGAATATTCGCCGCAATGCTGCCGCCCGCCATGCCAAATTTCAGCGGTTGCAGGCGCAGG  >  NC_000913/3676744‑3677016

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑