Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	566,245	0	G	A	37.7%	57.7 / 54.9	61	pseudogene (132/279 nt)	exoD	pseudogene, DLP12 prophage, phage‑type exonuclease family,Phage or Prophage Related
Reads supporting (aligned to +/- strand):  ref base G (17/21);  new base A (12/11);  total (29/32)
Fisher's exact test for biased strand distribution p-value = 6.07e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.09e-01
Rejected as consensus: Frequency below/above cutoff threshold.

GCTCCCCAAATACAAAACCAATTTCAGCCAGTGCCTCGTCCATTTTTTCGATGAACTCCGGCACCATCTCGTCAAAACTCGCCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGT  >  NC_000913/566109‑566382                                                                                                                                         |                                                                                                                                          gCTCCCCAAATACAAAACCAATTTCAGCCAGTGCCTCTTCCATTTTTTCGATGAACTCCGGCACCATCTCGTCAAAACTCGCCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATgcg                                                                                                                                         <  2:535454/139‑1 (MQ=255) gCTCCCCAAATACAAAACCAATTTCAGCCAGTGCCTCGTCCATTTTTTCGATGAACTCCGGCACCATCTCGTCAAAACTCGCCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATgcg                                                                                                                                         <  2:203541/139‑1 (MQ=255) gCTCCCCAAATACAAAACCAATTTCAGCCAGTGCCTCGTCCATTTTTTCGATGAACTCCGGCACCATCTCGTCAAAACTCGCCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATgcg                                                                                                                                         <  2:255969/139‑1 (MQ=255) gCTCCCCAAATACAAAACCAATTTCAGCCAGTGCCTCGTCCATTTTTTCGATGAACTCCGGCACCATCTCGTCAAAACTCGCCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATgcg                                                                                                                                         <  2:21204/139‑1 (MQ=255) gCTCCCCAAATACAAAACCAATTTCAGCCAGTGCCTCGTCCATTTTTTCGATGAACTCCGGCACCATCTCGTCAAAACTCGCCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATgcg                                                                                                                                         <  2:708590/139‑1 (MQ=255)     cccAAATACAAAACCAATTTCAGCCAGTGCCTCGTCCATTTTTTCGATGAACTCCGGCACCATCTCGTCAAAACTCGCCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCggg                                                                                                                                     <  2:203859/139‑1 (MQ=255)               aaaCCAATTTCAGCCACTGCCTCGTCCATTTTTTCGATGCACTCCGGCACGATCTCGTCAAAACTCGCCATGTACTTTTCATCCCGCTCAATCACGAAATAATGCAGGCCTTCACGACACATACGCGGCACATAGTTgg                                                                                                                           >  2:431628/1‑139 (MQ=255)                 aCCAATTTGAGCCAGTGCCTCGTCCATTTTTTCGATGAACTCCGGCACGATCTCGTCAAAACTCGCCATGTACTTTCCATCCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATACGCGGGTCATAGTTGGCa                                                                                                                         <  1:498569/139‑1 (MQ=255)                 aCCAATTTCAGCCAGTGCATCGTCCATTTTTTCGATGCACTCCGGCACCATCTCGTCAAAACTCGCCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCa                                                                                                                         <  2:113318/139‑1 (MQ=255)                  ccAATTTCAGCCAGTGCCTCGTCCATTTTTTCGATGAACTCCGGCACCATCTCGTCAAAACTCGCCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCaa                                                                                                                        >  1:420904/1‑139 (MQ=255)                          aGCCAGTGCCTCGTCCATTTTTTCGATGAACTCCGGCACCATCTCGTCAAAACTCGCCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGt                                                                                                                                    <  2:458929/119‑1 (MQ=255)                          aGCCAGTGCCTCGTCCATTTTTTCGATGAACTCCGGCACCATCTCGTCAAAACTCGCCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGt                                                                                                                                    >  1:458929/1‑119 (MQ=255)                                         cATTTTTTCGATGAACTCCGGCACCATCTCGTCAAAACTCGCCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTc                                                                                                 <  2:420904/139‑1 (MQ=255)                                                  gATGAACTCCGGCACCATCTCGTCAAAACTCGCCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATg                                                                                        <  2:614853/139‑1 (MQ=255)                                                  gATGAACTCCGGCACCATCTCGTCAAAACTCGCCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATg                                                                                        <  1:41443/139‑1 (MQ=255)                                                  gATGAACTCCGGCACCATCTCGTCAAAACTCGCCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATg                                                                                        <  1:180713/139‑1 (MQ=255)                                                        cTCCGGCACGATCTCGTCAAAACTCGCCATGTACTTTTCATCCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATACGCGGGTCATAGTTGGCAAAGTACCAGGCATTTTTTCGCGTCACCCACATGCTGTAc                                                                                  <  1:344728/139‑1 (MQ=255)                                                             gCACCATCTCGTCAAAACTCGCCATGTACTTTTCGTTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCAc                                                                             <  2:741481/139‑1 (MQ=255)                                                                cgaTCTCGTCAAAACTCGCCATGTGCTTTTCATCCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATACGCGGGt                                                                                                                                    >  2:568517/3‑81 (MQ=255)                                                                cgaTCTCGTCAAAACTCGCCATGTACTTTTCATCCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATACGCGGGt                                                                                                                                    <  1:568517/79‑1 (MQ=255)                                                                 gaTCTCGTCAAAACTCGCCATGTACTTTTCATCCCGCTCAATCACGACATAATGCAGGCCTTCACGATTCATACGCGGGTCATAGTTGGCAAAGTACCCGGCATTTTTTCGCGTGACCCACATGGTGTACTACACCTgg                                                                         <  1:511869/138‑1 (MQ=255)                                                                  aTCTCGTCAAAACTCGCCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTggg                                                                        >  1:597268/1‑139 (MQ=255)                                                                   tctcGTCAAAACTCGCCATGTACTTTTCATCCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATACGCGGGTCATAGTTGGCAAAGTACCAGGCATTTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGc                                                                       >  1:526432/1‑139 (MQ=255)                                                                                 gCCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCcaca                                                                                          >  2:112927/1‑106 (MQ=255)                                                                                 gCCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCcaca                                                                                          <  1:112927/106‑1 (MQ=255)                                                                                 gCCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCt                                                                                      <  2:347090/110‑1 (MQ=255)                                                                                 gCCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCt                                                                                      >  1:347090/1‑110 (MQ=255)                                                                                            ttCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATg                                                                                        >  1:175116/1‑97 (MQ=255)                                                                                            ttCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATg                                                                                        <  2:175116/97‑1 (MQ=255)                                                                                               aTTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATgcg                                                                                                                                         >  2:214320/1‑45 (MQ=255)                                                                                               aTTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATgcg                                                                                                                                         <  1:214320/45‑1 (MQ=255)                                                                                                 tCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTa                                                      <  1:160240/126‑1 (MQ=255)                                                                                                 tCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTa                                                      >  2:160240/1‑126 (MQ=255)                                                                                                 cccGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATacg                                                                                                                                         >  2:342178/2‑43 (MQ=255)                                                                                                 cccGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATACGCGGGTCATAGTTGGCAAAGTACCAGGCATTTTTTcg                                                                                                     <  2:434711/78‑1 (MQ=255)                                                                                                 cccGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATACGCGGGTCATAGTTGGCAAAGTACCAGGCATTTTTTcg                                                                                                     >  1:434711/2‑79 (MQ=255)                                                                                                 cccGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATACGCGGGTCATAGTTGGCAAAGTACCAGGCATTTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAg                                                               <  2:243370/116‑1 (MQ=255)                                                                                                 cccGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATACGCGGGTCATAGTTGGCAAAGTACCAGGCATTTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAg                                                               >  1:243370/2‑117 (MQ=255)                                                                                                    gCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCt                                                <  1:11407/129‑1 (MQ=255)                                                                                                    gCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCt                                                >  2:11407/1‑129 (MQ=255)                                                                                                    gCTCAATCACGACATAATGCAGGCCTTCACGCTTCATACGCGGGTCATAGTTGGCAAAGTACCAGGCATTTTTTCGCGTCACCCACATg                                                                                        >  1:340869/1‑89 (MQ=255)                                                                                                    gCTCAATCACGACATAATGCAGGCCTTCACGCTTCATACGCGGGTCATAGTTGGCAAAGTACCAGGCATTTTTTCGCGTCACCCACATg                                                                                        <  2:340869/89‑1 (MQ=255)                                                                                                     cTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCg                                     >  2:691249/1‑139 (MQ=255)                                                                                                                  tAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGt                                                                                                                              <  1:242239/37‑1 (MQ=255)                                                                                                                  tAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGt                                                                                                                              >  2:242239/1‑37 (MQ=255)                                                                                                                   aaTGCAGGCCTTCACGCTTCATACGCGGGTCATAGTTGGCAAAGTACCAGGCATTTTTTCGCGTCAccc                                                                                             >  1:654029/1‑69 (MQ=255)                                                                                                                   aaTGCAGGCCTTCACGCTTCATACGCGGGTCATAGTTGGCAAAGTACCAGGCATTTTTTCGCGTCAccc                                                                                             <  2:654029/69‑1 (MQ=255)                                                                                                                        aGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCAccc                                                                                             <  2:40775/64‑1 (MQ=255)                                                                                                                        aGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCAccc                                                                                             >  1:40775/1‑64 (MQ=255)                                                                                                                          gCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCcac                                                                                           <  2:801591/64‑1 (MQ=255)                                                                                                                          gCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCcac                                                                                           >  1:801591/1‑64 (MQ=255)                                                                                                                          gCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAAt                  <  1:670644/137‑1 (MQ=255)                                                                                                                          gCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAAt                  <  1:709391/137‑1 (MQ=255)                                                                                                                          gCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAAt                  >  2:709391/1‑137 (MQ=255)                                                                                                                          gCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAAt                  >  2:670644/1‑137 (MQ=255)                                                                                                                           ccTTCACGCTTCATACGCGGGTCATAGTTGGCAAAGTACCAGGCATTTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCTGACTTTATGGCCTCGAAACCACCGAGCCGGAACTTCATGAAATccc               >  1:733003/1‑139 (MQ=255)                                                                                                                             ttCACGCTTCATACGCGGGTCATAGTTGGCAAAGTACCAGGCATTTTTTCGCGt                                                                                                  <  2:834763/54‑1 (MQ=255)                                                                                                                             ttCACGCTTCATACGCGGGTCATAGTTGGCAAAGTACCAGGCATTTTTTCGCGt                                                                                                  >  1:834763/1‑54 (MQ=255)                                                                                                                             ttCACGCTTCATACGCGGGTCATAGTTGGCAAAGTACCAGGCATTTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCTGACTTTAtggc                                                  >  1:337505/1‑99 (MQ=255)                                                                                                                             ttCACGCTTCATACGCGGGTCATAGTTGGCAAAGTACCAGGCATTTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCTGACTTTAtggc                                                  <  2:337505/102‑4 (MQ=255)                                                                                                                             tccACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTAGCGTCACCCACATGCTGTACTGCACCTGGGCCAAGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAAACGCgg             >  2:290948/3‑139 (MQ=255)                                                                                                                              tCATGCTTCATGGGCGGCACATAGTGAGCAAAATACCAGACATCTTTTCGCGTCACCCACATACTGGACTGCTCCTGAGCCATGTAAGCCGATTTTATGGCCTCGAAACCACCGAACCGGAATTTCATTAAATCCCggg            <  2:416753/139‑1 (MQ=255)                                                                                                                              tCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCggg            <  2:597268/139‑1 (MQ=255)                                                                                                                              tCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCggg            <  1:691249/139‑1 (MQ=255)                                                                                                                               cACGCTTCATACGCGGGTCATAGTTGGCAAAGTACCAGGCATTTTTTCGCGTCACCCACATGCTGTACTGCACCTggg                                                                        <  2:655084/78‑1 (MQ=255)                                                                                                                               cACGCTTCATACGCGGGTCATAGTTGGCAAAGTACCAGGCATTTTTTCGCGTCACCCACATGCTGTACTGCACCTggg                                                                        >  1:655084/1‑78 (MQ=255)                                                                                                                               cACGCTTCATACGCGGGTCATAGTTGGCAAAGTACCAGGCATTTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCTGACTTTATGGCCTCGAAACCACCGAGCCGGAACTTCATGAAATCCCGGGa           >  1:564402/1‑139 (MQ=255)                                                                                                                               cACGCTTCATACGCGGGTCATAGTTGGCAAAGTACCAGGCATTTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCTGACTTTATGGCCTCGAAACCACCGAGCCGGAACTTCATGAAATCCCGGGa           >  1:57386/1‑139 (MQ=255)                                                                                                                                    ttCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATg                                                                                        >  2:595647/1‑57 (MQ=255)                                                                                                                                    ttCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATg                                                                                        <  1:595647/57‑1 (MQ=255)                                                                                                                                        tGCGCGGGTCATAGTTGGCAAAGTACCAGCCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCCCCGAGCCGGAATTTCCTGAAATCCCGGGAGGAACGaat  >  1:416753/1‑137 (MQ=255)                                                                                                                                         gcgcGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGt                                                                                                  >  1:419658/1‑42 (MQ=255)                                                                                                                                         gcgcGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGt                                                                                                  <  2:419658/42‑1 (MQ=255)                                                                                                                                         |                                                                                                                                          GCTCCCCAAATACAAAACCAATTTCAGCCAGTGCCTCGTCCATTTTTTCGATGAACTCCGGCACCATCTCGTCAAAACTCGCCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGT  >  NC_000913/566109‑566382

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑