Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 571,444 G→A *184* (TAG→TAA ybcL → inactive polymorphonuclear leukocyte migration suppressor, DLP12 prophage, UPF0098 family secreted protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913571,4440GA100.0% 107.5 / NA 34*184* (TAG→TAAybcLinactive polymorphonuclear leukocyte migration suppressor, DLP12 prophage, UPF0098 family secreted protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (18/16);  total (18/16)

TGAAAAGATTCCTGTAGATTCTAACTCCAGCGGAGCGTTAGTTGGTTATATGCTTAATGCTAATAAAATCGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAGGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCGAAGAAATACGGATGCATAAACATCATGAGATTCATAGGGTTAAGCCTCTTATGCCAGCTTTGTGTCGTATCCGTCAGGGAAAGAAAGTT  >  NC_000913/571336‑571576
                                                                                                            |                                                                                                                                    
tGAAAAGATTCCTGTAGATTCTAACTCCAGCGGAGCGTTAGTTGGTTATATGCTTAATGCTAATAAAATCGCAACCGCTGAGATAACACCAGTTTATGGGATAAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACt                                                                                                        <  1:212488/139‑1 (MQ=255)
              tAGATTCTAACTCCAGCGGAGCGTTAGTTGGTTATATGCTTAATGCTAATAAAATCGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCga                                                                                          <  1:516168/139‑1 (MQ=255)
                   tCTAACTCCAGCGGAGCGTTAGTTGGTTATATGCTTAATGCTAATAAAATCGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCGAAGaaa                                                                                     >  1:378155/1‑139 (MQ=255)
                       aCTCCAGCGGAGCGTTAGTTGGTTATATGCTTAATGCTAATAAAATCGCAACTGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCGAAGAAATACg                                                                                 <  1:554308/139‑1 (MQ=255)
                           cAGCGGAGCGTTAGTTGGTTATATGCTTAATGCTAATAAAATCGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCGAAGAAATACGGATg                                                                             >  2:89139/1‑139 (MQ=255)
                             gCGGAGCGTTAGTTGGTTATATGCTTAATGCTAATAAAATCGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGCaa                                                                                                                   <  1:204031/99‑1 (MQ=255)
                             gCGGAGCGTTAGTTGGTTATATGCTTAATGCTAATAAAATCGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGCaa                                                                                                                   >  2:204031/1‑99 (MQ=255)
                             gCGGAGCGTTAGTTGGTTATATGCTTAATGCTAATAAAATCGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCGAAGAAATACGGATGCa                                                                           >  1:531755/1‑139 (MQ=255)
                                                  tGCTTAATGCTAATAAAATCGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGTgaga                                                                                                                               <  2:664748/66‑1 (MQ=255)
                                                  tGCTTAATGCTAATAAAATCGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGTgaga                                                                                                                               >  1:664748/1‑66 (MQ=255)
                                                  tGCTTAATGCTAATAAAATCGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGCAAGAGGt                                                                                                              <  1:28981/83‑1 (MQ=255)
                                                  tGCTTAATGCTAATAAAATCGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGCAAGAGGt                                                                                                              >  2:28981/1‑83 (MQ=255)
                                                         tGCTAATAAAATCGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGTGAGAGTATGCTAGCAAGAGGTAAGACTAACTTAAAGATCGAAGAAATACGGATGCATAAACATCATGAGATTCATAGGGTTAAg                                               <  1:509100/139‑1 (MQ=255)
                                                            taataaAATCGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCGAAGAAATACGGATGCATAAACATCATGAGATTCATAGGGTTAAGCct                                            >  2:162601/1‑139 (MQ=255)
                                                              ataaAATCGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCGAAGAAATACGGATGCATAAAcatcat                                                                 >  2:668330/1‑116 (MQ=255)
                                                              ataaAATCGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCGAAGAAATACGGATGCATAAAcatcat                                                                 <  1:668330/116‑1 (MQ=255)
                                                              ataaAATCGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCGAAGAAATACGGATGCATAAACATCATGAGATTCATAGGGTTAAGCctct                                          >  1:451880/1‑139 (MQ=255)
                                                                   aTCGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCGAAGAAATACGGATGCATAAACATCATGAGATTCATAGGGTTAAGCCTCTTATGc                                     <  1:32543/139‑1 (MQ=255)
                                                                   aTCGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCGAAGAAATACGGATGCATAAACATCATGAGATTCATAGGGTTAAGCCTCTTATGc                                     >  2:395731/1‑139 (MQ=255)
                                                                   aTCGCAACCACTGAGATAACACCAGTTTATGAGATGAAGTAAGGTGAGAGTATTCTGGCAAGAGGTAAGACTAACTTAAAGATCGAAGAAATACGGATGCATAAACATCATGAGATTCATAGGGTTAAGCCTCTTATGc                                     <  2:451880/139‑1 (MQ=255)
                                                                    tCGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCGAAGAAATACGGATGCATAAACATCATGAGATTCATAGGGTTAAGCCTCTTATGcc                                    >  1:212086/1‑139 (MQ=255)
                                                                       cAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCgaagaga                                                                                     >  1:87940/1‑85 (MQ=255)
                                                                       cAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCGAAGaaa                                                                                     <  2:87940/87‑1 (MQ=255)
                                                                        aaCCGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCGAAGAAATACGGa                                                                               >  2:508204/1‑92 (MQ=255)
                                                                        aaCCGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCGAAGAAATACGGa                                                                               <  1:508204/92‑1 (MQ=255)
                                                                                  aTAACACCAGTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCGAAGAAATACGGATGCATAAACATCATGAGATTCATAGGGTTAAGCCTCTTATGCCAGCTTTGTGTCGTa                      <  2:439014/139‑1 (MQ=255)
                                                                                          aGTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCGAAGAAATACGGATGCATAAACATCATGAGATTCAt                                                        >  2:659956/1‑97 (MQ=255)
                                                                                          aGTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCGAAGAAATACGGATGCATAAACATCATGAGATTCAt                                                        <  1:659956/97‑1 (MQ=255)
                                                                                           gTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCGAAGAAATACGGATGCATAAACATCATGAGATTCATAGGGTTAAGCCTCTTATGCCAGCTTTGTGTCGTATCCGTCAgg             >  2:771915/1‑139 (MQ=255)
                                                                                              tATGAGATAAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCga                                                                                          >  2:541356/1‑59 (MQ=255)
                                                                                              tATGAGATAAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCga                                                                                          <  1:541356/59‑1 (MQ=255)
                                                                                                      aaaGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACtt                                                                                                   >  1:70946/1‑42 (MQ=255)
                                                                                                      aaaGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACtt                                                                                                   <  2:70946/42‑1 (MQ=255)
                                                                                                      aaaGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCGAAGAAATACGGATGCATAAACATCATGAGATTCATAGGGTTAAGCCTCTTATGCCAGCTTTGTGTCGTATCCGTCAGGGAAAGAAAGtt  >  1:742779/1‑139 (MQ=255)
                                                                                                            |                                                                                                                                    
TGAAAAGATTCCTGTAGATTCTAACTCCAGCGGAGCGTTAGTTGGTTATATGCTTAATGCTAATAAAATCGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAGGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCGAAGAAATACGGATGCATAAACATCATGAGATTCATAGGGTTAAGCCTCTTATGCCAGCTTTGTGTCGTATCCGTCAGGGAAAGAAAGTT  >  NC_000913/571336‑571576

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: