Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 677,889 G→A A159T (GCC→ACC)  djlB → putative HscC co‑chaperone, uncharacterized J domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913677,8890GA100.0% 83.4 / NA 27A159T (GCC→ACC) djlBputative HscC co‑chaperone, uncharacterized J domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (16/11);  total (16/11)

CACAATCGCCATGAACACTGCCCATTTATCCTTCGAGTGCGTGGTGTTATTAGCAGAAAGATTGCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATGCCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATGACACCATCGATTTTTACCAAATGTTCGCTCGGATTTGGTCATCGCATCCACAATGGCTGACATTGTATT  >  NC_000913/677762‑678024
                                                                                                                               |                                                                                                                                       
cacaATCGCCATGAACACTGCCCATTTATCCTTCGAGTGCGTGGTGTTATTAGCAGAAAGATTGCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATAccctgtctctt                                                                                                                              >  1:741479/1‑130 (MQ=255)
        ccATGAACACTGCCCATTTATCCTTCGAGTGCGTGGTGTTATTAGCAGAAAGATTGCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATACCATTGCGAAGGGGAATGt                                                                                                                      >  2:168197/1‑139 (MQ=255)
           tGAACACTGCCCATTTATCCTTCGAGTGCGTGGTGTTATTAGCAGAAAGATTGCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATACCATTGCGAAGGGGAATGtttt                                                                                                                   >  2:543324/1‑139 (MQ=255)
               cacTGCCCATTTATCCTTCGAGTGCGTGGTGTTATTAGCAGAAAGATTGCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATACCATTGCGAAGGGGAATGTTTTTa                                                                                                                 >  2:731612/1‑137 (MQ=255)
               cacTGCCCATTTATCCTTCGAGTGCGTGGTGTTATTAGCAGAAAGATTGCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATACCATTGCGAAGGGGAATGTTTTTa                                                                                                                 <  1:731612/137‑1 (MQ=255)
                      cATTTATCCTTCGAGTGCGTGGTGTTATTAGCAGAAAGATTGCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATACCATTGCGAAGGGGAATGTTTTTAACTTCCAGa                                                                                                        <  1:135225/139‑1 (MQ=255)
                                       cGTGGTGTTATTAGCAGAAAGATTGCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATACCATTGCGAAGGGGa                                                                                                                          <  1:300304/104‑1 (MQ=255)
                                       cGTGGTGTTATTAGCAGAAAGATTGCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATACCATTGCGAAGGGGa                                                                                                                          >  2:300304/1‑104 (MQ=255)
                                                     cAGAAAGATTGCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATACCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCaaaa                                                                         >  1:426512/1‑139 (MQ=255)
                                                      aGAAAGATTGCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATACCATTGCGAAGGGGAATGTTTTTAACTTCCAGa                                                                                                        >  1:29231/1‑107 (MQ=255)
                                                      aGAAAGATTGCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATACCATTGCGAAGGGGAATGTTTTTAACTTCCAGa                                                                                                        <  2:29231/107‑1 (MQ=255)
                                                               gCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATACCATTGCGAAGGGGAATGTTTTTAACTTCCAGa                                                                                                        <  1:423836/98‑1 (MQ=255)
                                                               gCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATACCATTGCGAAGGGGAATGTTTTTAACTTCCAGa                                                                                                        >  2:423836/1‑98 (MQ=255)
                                                               gCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATACCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATGACACCATc                                                               >  1:209702/1‑139 (MQ=255)
                                                                               gaAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATACCATTGCGAAGGGGAATGTTTTTAACTTCCAGAc                                                                                                       <  2:391468/83‑1 (MQ=255)
                                                                               gaAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATACCATTGCGAAGGGGAATGTTTTTAACTTCCAGAc                                                                                                       >  1:391468/1‑83 (MQ=255)
                                                                               gaAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATACCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCCGCATCTGCCCGTTGCCGTGCAAAATGACACCATCGATTTTTACCAAATGt                                               >  1:273450/1‑139 (MQ=255)
                                                                                         gggAAATAGACGAAGAAGAACTGGAATCCTTTTTATATACCATTGCGAAGGGGa                                                                                                                          >  2:162660/1‑54 (MQ=255)
                                                                                         gggAAATAGACGAAGAAGAACTGGAATCCTTTTTATATACCATTGCGAAGGGGa                                                                                                                          <  1:162660/54‑1 (MQ=255)
                                                                                          ggAAATAGACGAAGAAGAACTGGAATCCTTTTTATATACCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTg                                                                                   >  1:697645/1‑92 (MQ=255)
                                                                                          ggAAATAGACGAAGAAGAACTGGAATCCTTTTTATATACCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTg                                                                                   <  2:697645/92‑1 (MQ=255)
                                                                                          ggAAATAGACGAAGAAGAACTGGAATCCTTTTTATATACCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATGACACCATCGATTTTTACCAAATGTTCGCTCGGAtt                                    >  1:788370/1‑139 (MQ=255)
                                                                                                  aCGAAGAAGAACTGGAATCCTTTTTATATACCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATGACACCATCGATTTTTACCAAATGTTCGCTCGGATTTGGTCATc                            <  1:543324/139‑1 (MQ=255)
                                                                                                     aagaagaaCTGGAATCCTTTTTATATACCATTGCGAAGGGGAATGTTTTTAACTTCCAGa                                                                                                        <  1:329067/60‑1 (MQ=255)
                                                                                                     aagaagaaCTGGAATCCTTTTTATATACCATTGCGAAGGGGAATGTTTTTAACTTCCAGa                                                                                                        >  2:329067/1‑60 (MQ=255)
                                                                                                                  aTCCTTTTTATATACCATTGCGAAGGGGAATGTTTTTAACTTCCAGAc                                                                                                       >  1:189316/1‑48 (MQ=255)
                                                                                                                  aTCCTTTTTATATACCATTGCGAAGGGGAATGTTTTTAACTTCCAGAc                                                                                                       <  2:189316/48‑1 (MQ=255)
                                                                                                                            tataCCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATGACACCATCGATTTTTACCAAATGTTCGCTCGGATTTGGTCATCGCATCCACAATGGCTGACATTGTAtt  <  2:447159/139‑1 (MQ=255)
                                                                                                                               |                                                                                                                                       
CACAATCGCCATGAACACTGCCCATTTATCCTTCGAGTGCGTGGTGTTATTAGCAGAAAGATTGCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATGCCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATGACACCATCGATTTTTACCAAATGTTCGCTCGGATTTGGTCATCGCATCCACAATGGCTGACATTGTATT  >  NC_000913/677762‑678024

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: