Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 679,874 G→A G123R (GGG→AGG)  ybeU → DUF1266 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913679,8740GA100.0% 106.0 / NA 34G123R (GGG→AGG) ybeUDUF1266 family protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (17/17);  total (17/17)

CTGGTGGCTCGGAGAGAGGGTTGATTTATACCCAATTCGTTGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGGGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGTTTATGGCTGCAATCGCGCGTCTATGTACGAGCTCACCATTACTATCACAGCTGGATGCACTATTTT  >  NC_000913/679773‑679993
                                                                                                     |                                                                                                                       
cTGGTGGCTCGGAGAGAGGGTTGATTTATACCCAATTCGTTGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCt                                                                                    <  2:457590/139‑1 (MQ=255)
                  ggTTGATTTATACCCAATTCGTTGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGttt                                                                  <  2:645239/139‑1 (MQ=255)
                            taCCCAATTCGTTGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGAATCAAAGCCTGGGATTCTGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGTTTATGGCTGCaa                                                        >  2:548362/1‑139 (MQ=255)
                             aCCCAATTCGTTGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGTTTATGGCTGCAAt                                                       >  2:802647/1‑139 (MQ=255)
                                   ttCGTTGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGTTTATGGCTGCAATcgcgcg                                                 >  1:461914/1‑139 (MQ=255)
                                    tCGTTGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGTTTATGGCTGCAATCGCGCGt                                                <  2:461914/139‑1 (MQ=255)
                                               aCCGCTATGTGCTGTGGGGAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGTAAGAAGAGAGTTTATGGCTGCAATCGCGCGTCTATGTACGAg                                     <  1:548362/139‑1 (MQ=255)
                                                     aTGTGCTGTGGGGAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGTTTATGGCTGCAATCGCGCGTCTATGTACGAGCTCAcc                               >  2:386438/1‑139 (MQ=255)
                                                              ggggAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACg                                                                                                            >  1:460132/1‑53 (MQ=255)
                                                              ggggAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACg                                                                                                            <  2:460132/53‑1 (MQ=255)
                                                              ggggAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCg                                                                                                       >  1:501746/1‑58 (MQ=255)
                                                              ggggAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCg                                                                                                       <  2:501746/58‑1 (MQ=255)
                                                              ggggAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCt                                                                                    <  1:325142/77‑1 (MQ=255)
                                                              ggggAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCt                                                                                    >  2:325142/1‑77 (MQ=255)
                                                                ggAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGTTTATGGCTGCAATCGCGCGt                                                >  1:437446/1‑111 (MQ=255)
                                                                ggAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGTTTATGGCTGCAATCGCGCGt                                                <  2:437446/111‑1 (MQ=255)
                                                                  aaGGGGGAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGTTTATGGCTGCAATCGCGCGTCTATGTACGAGCt                                   <  1:627731/122‑1 (MQ=255)
                                                                  aaGGGGGAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGTTTATGGCTGCAATCGCGCGTCTATGTACGAGCt                                   >  2:627731/1‑122 (MQ=255)
                                                                  aaGGGGGAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGTTTATGGCTGCAATCGCGCGTCTATGTACGAGCTCAcc                               <  2:525629/126‑1 (MQ=255)
                                                                  aaGGGGGAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGTTTATGGCTGCAATCGCGCGTCTATGTACGAGCTCAcc                               >  1:525629/1‑126 (MQ=255)
                                                                      gggAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGa                                                                                  <  1:647606/71‑1 (MQ=255)
                                                                      gggAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGa                                                                                  >  2:647606/1‑71 (MQ=255)
                                                                       ggAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGTTTATGGCTGCAATCGCGCGTCTATGTACGAGCTCACCATTACTATCACAGCTGGa             >  1:263304/1‑139 (MQ=255)
                                                                             aaaGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGTTTATGGCTGCAATCGCGCGTCTATGTACGAg                                     >  1:51189/1‑109 (MQ=255)
                                                                             aaaGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGTTTATGGCTGCAATCGCGCGTCTATGTACGAg                                     <  2:51189/109‑1 (MQ=255)
                                                                                  cTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGTTTATGGCTGCAATCGCGCGTCTATGCACGAGCTCACCATTACTATCACAGCTGGATGCTCTAtttt  <  1:512070/139‑1 (MQ=255)
                                                                                     ggATTATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACg                                                                                <  2:175015/58‑1 (MQ=255)
                                                                                     ggATTATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACg                                                                                >  1:175015/1‑58 (MQ=255)
                                                                                     ggATTATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGTTTATGGCTGCAATCGCGCGTCTATGTACGAGCTCACCATTACTATCACAGCTGGATg           <  2:378394/127‑1 (MQ=255)
                                                                                     ggATTATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGTTTATGGCTGCAATCGCGCGTCTATGTACGAGCTCACCATTACTATCACAGCTGGATg           >  1:378394/1‑127 (MQ=255)
                                                                                               cGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGTTTATGGCTGCAATCGCGCGTCTATGTACGAGCTCAcc                               >  1:126654/1‑97 (MQ=255)
                                                                                               cGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGTTTATGGCTGCAATCGCGCGTCTATGTACGAGCTCAcc                               <  2:126654/97‑1 (MQ=255)
                                                                                                 aaTGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGTTTATGGCTGCAATcgcg                                                   <  1:7155/75‑1 (MQ=255)
                                                                                                 aaTGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGTTTATGGCTGCAATcgcg                                                   >  2:7155/1‑75 (MQ=255)
                                                                                                     |                                                                                                                       
CTGGTGGCTCGGAGAGAGGGTTGATTTATACCCAATTCGTTGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGGGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGTTTATGGCTGCAATCGCGCGTCTATGTACGAGCTCACCATTACTATCACAGCTGGATGCACTATTTT  >  NC_000913/679773‑679993

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: