Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 775,120 G→A A123A (GCG→GCA ybgC → acyl‑CoA thioester hydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913775,1200GA100.0% 97.5 / NA 32A123A (GCG→GCAybgCacyl‑CoA thioester hydrolase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (17/15);  total (17/15)

ACTGAAATAACATCAATGCGTGGCACCTCTTTGGTTTTCACGCAACGTATTGTCAACGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCGCTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGATTTGTTCCTGAAGGCTAGCCTTCTGGTTAAACTTATCATGTTGATTTTGATTGGTTTTTCAATCGCATCTTGGGC  >  NC_000913/774989‑775250
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aCTGAAATAACATCAATGCGTGGCACCTCTTTGGTTTTCACGCAACGTATTGTCAACGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCa                                                                                                                             >  1:170601/1‑139 (MQ=255)
          cATCAATGCGTGGCACCTCTTTGGTTTTCACGCAACGTATTGTCAACGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGt                                                                                                                   >  1:175792/1‑139 (MQ=255)
              aaTGCGTGGCACCTCTTTGGTTTTCACGCAACGTATTGTCAACGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTcgcg                                                                                                               <  2:784337/139‑1 (MQ=255)
              aaTGCGTGGCACCTCTTTGGTTTTCACGCAACGTATTGTCAACGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTcgc                                                                                                                <  1:832720/138‑1 (MQ=255)
              aaTGCGTGGCACCTCTTTGGTTTTCACGCAACGTATTGTCAACGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTcgc                                                                                                                >  2:832720/1‑138 (MQ=255)
              aaTGCGTGGCACCTCTTTGGTTTTCACGCAACGTATTGTCAACCCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTcgcg                                                                                                               >  1:336664/1‑139 (MQ=255)
                   gTGGCACCTCTTTGGTTTTCACGCAACGTATTGTCAACGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTCGCGGAGtt                                                                                                          >  1:328418/1‑139 (MQ=255)
                   gTGGCACCTCTTTGGTTTTCACGCAACGTATTGTCAACCCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTCGCGGAGtt                                                                                                          <  2:336664/139‑1 (MQ=255)
                                  ttttCACGCAACGTATTGTCAACGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGtgactgac                                                                                           <  1:686204/139‑1 (MQ=255)
                                                       aCGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACGTGAATATCCTTGATTTGTTc                                                                      >  1:316097/1‑139 (MQ=255)
                                                         gCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGATTTGTTCCt                                                                    >  2:459709/1‑139 (MQ=255)
                                                         gCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGATTTGTTCCt                                                                    >  1:752237/1‑139 (MQ=255)
                                                              gaATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGATTTGTTCCTGAAgg                                                               >  2:92556/1‑139 (MQ=255)
                                                                      tGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGtgactga                                                                                            <  1:156784/102‑1 (MQ=255)
                                                                      tGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGtgactga                                                                                            >  2:156784/1‑102 (MQ=255)
                                                                                    gagGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGATTTGTTCCTGAAGGCTAGCCTTCTGGTTAAACTTAt                                         <  1:410560/139‑1 (MQ=255)
                                                                                     agGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGt                                                                                                  <  1:6134/81‑1 (MQ=255)
                                                                                     agGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGt                                                                                                  >  2:6134/1‑81 (MQ=255)
                                                                                     agGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGATTTGTTCCTGAAGGCTAGCCTTCTGGTTAAACTTa                                          <  1:705819/137‑1 (MQ=255)
                                                                                     agGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGATTTGTTCCTGAAGGCTAGCCTTCTGGTTAAACTTa                                          >  2:705819/1‑137 (MQ=255)
                                                                                                 tttGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGAt                                                                            >  1:676385/1‑91 (MQ=255)
                                                                                                 tttGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGAt                                                                            <  2:676385/91‑1 (MQ=255)
                                                                                                       ttGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGtga                                                                                                <  1:394814/65‑1 (MQ=255)
                                                                                                       ttGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGtga                                                                                                >  2:394814/1‑65 (MQ=255)
                                                                                                       ttGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGATTTGTTCCTGAAGGCTAGCCTTCTGGTTAAACTTATCa                                       <  2:467169/122‑1 (MQ=255)
                                                                                                       ttGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGATTTGTTCCTGAAGGCTAGCCTTCTGGTTAAACTTATCa                                       >  1:467169/1‑122 (MQ=255)
                                                                                                          aCCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGa                                                                                       <  2:262686/71‑1 (MQ=255)
                                                                                                          aCCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGa                                                                                       >  1:262686/1‑71 (MQ=255)
                                                                                                                           ccTCGTGCACTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGATTTGTTCCTGAAGGCTAGCCTTCTGGTTAAACTTATCATGTTGATTTTGATTGGTTTTTCAATCGCATCTTGGGc  <  2:175792/139‑1 (MQ=255)
                                                                                                                           ccTCGTGCACTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGATTTGTTCCTGAAGGCTAGCCTTCTGGTTAAACTTATCATGTTGATTTTGATTGGTTTTTCAATCGCATCTTGGGc  <  1:92556/139‑1 (MQ=255)
                                                                                                                              cGTGCACTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGATTTGTTCCTGAAGGCTAGCCTTCTGGTTAAACTTATCATGTTGATTTTGATTGGTTTTTCAATCGCATCt       <  2:417421/131‑1 (MQ=255)
                                                                                                                              cGTGCACTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGATTTGTTCCTGAAGGCTAGCCTTCTGGTTAAACTTATCATGTTGATTTTGATTGGTTTTTCAATCGCATCt       >  1:417421/1‑131 (MQ=255)
                                                                                                                                   |                                                                                                                                  
ACTGAAATAACATCAATGCGTGGCACCTCTTTGGTTTTCACGCAACGTATTGTCAACGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCGCTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGATTTGTTCCTGAAGGCTAGCCTTCTGGTTAAACTTATCATGTTGATTTTGATTGGTTTTTCAATCGCATCTTGGGC  >  NC_000913/774989‑775250

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: