Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	776,397	C→T	A19V (GCA→GTA)	tolA →	membrane anchored protein in TolA‑TolQ‑TolR complex

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	776,397	0	C	T	100.0%	114.9 / NA	36	A19V (GCA→GTA)	tolA	membrane anchored protein in TolA‑TolQ‑TolR complex
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (17/19);  total (17/19)

GCGTTTCCCTTGCTTGAAAGAGAGCGGGTAACAGGCGAACAGTTTTTGGAAACCGAGAGTGTCAAAGGCAACCGAACAAAACGACAAGCTCAAGCGGGCGATAATTATTTCAGCAGTGCTGCATGTCATCTTATTTGCGGCGCTGATCTGGAGTTCGTTCGATGAGAATATAGAAGCTTCAGCCGGAGGCGGCGGTGGTTCGTCCATCGACGCTGTCATGGTTGATTCAGGTGCGGTAGTTGAG  >  NC_000913/776284‑776527                                                                                                                  |                                                                                                                                   gCGTTTCCCTTGCTTGAAAGAGAGCGGGTAACAGGCGAACAGTTTTTGGAAACCGAGAGTGTCAAAGGCAACCGAACAAAACGACAAGCTCAAGCGGGCGATAATTATTTCAGTAGTGCTGCATGTCATCTTATTTgcg                                                                                                           >  2:818532/1‑139 (MQ=255)    tttCCCTTGCTTGAAAGAGAGCGGGTAACAGGCGAACAGTTTTTGGAAACCGAGAGTGTCAAAGGCAACCGAATAAAACGACAAGCTCAAGCGGGCGATAATTATTTCAGTAGTGCTGCATGTCATCTTATTTgcggcg                                                                                                        <  1:201046/139‑1 (MQ=255)    tttCCCTTGCTTGAAAGAGAGCGGGTAACAGGCGAACAGTTTTTGGAAACCGAGAGTGTCAAAGGCAACCGAACAAAACGACAAGCTCAAGCGGGCGATAATTATTTCAGTAGTGCTGCATGTCATCTTATTTgcggcg                                                                                                        <  1:95436/139‑1 (MQ=255)    tttCCCTGGCTTAAAAGAGGGCGGGTAACAGGCGAACAGTTTTTGGAAACGGAGAGTGTCAAAGGCAACCGAACAAAACGACAAGCTCAAGCGGGCGATAATTATTTCAGTAGTGCTGCATGTCATCTTATTTgcggcg                                                                                                        <  2:806912/139‑1 (MQ=255)      tCCCTTGCTTGAAAGAGAGCGGGTAACAGGCGAACAGTTTTTGGAAACCGAGAGTGTCAAAGGCAACCGAACAAAACGACAAGCTCAAGCGGGCGATAATTATTTCAGTAGTGCTGCATGTCATCTTATTTGCGGCGCt                                                                                                      <  1:179713/139‑1 (MQ=255)                 aaaGAGAGCGGGTAACAGGCGAACAGTTTTTGGAAACCGAGAGTGTCAAAGGCAACCGAACAAAACGACAAGCTCAAGCGGGCGATAATTATTTCAGTAGTGCTGCAt                                                                                                                          <  2:519663/108‑1 (MQ=255)                 aaaGAGAGCGGGTAACAGGCGAACAGTTATTGGAAACCGAGAGTGTCAAAGGCAACCGAACAAAACGACAAGCTCAAGCGGGCGATAATTATTTCAGTAGTGCTGCAt                                                                                                                          >  1:519663/1‑108 (MQ=255)                      gagCGGGTAACAGGCGAACAGTTTTTGGAAACCGAGAGTGTCAAAGGCAACCGAACAAAACGACAAGCTCAAGCGGGCGATAATTATTTCAGTAGTGCTGCATGTCATCTTATTTgcggcg                                                                                                        <  2:632183/121‑1 (MQ=255)                      gagCGGGTAACAGGCGAACAGTTTTTGGAAACCGAGAGTGTCAAAGGCAACCGAACAAAACGACAAGCTCAAGCGGGCGATAATTATTTCAGTAGTGCTGCATGTCATCTTATTTgcggcg                                                                                                        >  1:632183/1‑121 (MQ=255)                       agCGGGTAACAGGCGAACAGTTTTTGGAAACCGAGAGTGTCAAAGGCAACCGAACAAAACGACAAGCTCAAGCGGGCGATAATTATTTCAGTAGTGCTGCATGTCATCTTATTTGCGGCGCTGATCTGGAGttcgttcg                                                                                     >  1:425894/1‑139 (MQ=255)                       agCGGGTAACAGGCGAACAGTTTTTGGAAACCGAGAGTGTCAAAGGCAACCGAACAAAACGACAAGCTCAAGCGGGCGATAATTATTTCAGTAGTGCTGCATGTCATCTTATTTGCGGCGCTGATCTGGAGttcgttcg                                                                                     >  1:465618/1‑139 (MQ=255)                         cGGGTAACAGGCGAACAGTTTTTGGAAACCGAGAGTGTCAAAGGCAACCGAACAAAACGACAAGCTCAAGCGGGCGATAATTATTTCAGTAGTGCTGCATGTCATCTTATTTGCGGCGCTGATCTGGAGTTCGTTCGAt                                                                                   <  2:425894/139‑1 (MQ=255)                         cGGGTAACAGGCGAACAGTTTTTGGAAACCGAGAGTGTCAAAGGCAACCGAACAAAACGACAAGCTCAAGCGGGCGATAATTATTTCAGTAGTGCTGCATGTCATCTTATTTGCGGCGCTCATCTGGAGTTCGTTCGAt                                                                                   <  2:465618/139‑1 (MQ=255)                                      aaCAGTTTTTGGAAACCGAGAGTGTCAAAGGCAACCGAACAAAACGACAAGCTCAAGCGGGCGATAATTATTTCAGTAGTGCTGCATGTCATCTTATTTgcgg                                                                                                          <  2:800958/103‑1 (MQ=255)                                      aaCAGTTTTTGGAAACCGAGAGTGTCAAAGGCAACCGAACAAAACGACAAGCTCAAGCGGGCGATAATTATTTCAGTAGTGCTGCATGTCATCTTATTTgcgg                                                                                                          >  1:800958/1‑103 (MQ=255)                                                        agagTGTCAAAGGCAACCGAACAAAACGACAAGCTCAAGCGGGCGATAATTATTTCAGTAGTGCTGCATGTCATCTTATTTGCGGCGCTGATCTGGAgttcgt                                                                                        <  2:510878/103‑1 (MQ=255)                                                        agagTGTCAAAGGCAACCGAACAAAACGACAAGCTCAAGCGGGCGATAATTATTTCAGTAGTGCTGCATGTCATCTTATTTGCGGCGCTGATCTGGAgttcgt                                                                                        >  1:510878/1‑103 (MQ=255)                                                            tgtCAAAGGCAACCGAACAAAACGACAAGCTCAAGCGGGCGATAATTATTTCAGTAGTGCTGCATGTCATCTTATTTGCGGCGCTGATCTGGAGTTCGTTCGATGAGAATATAGAAGCTTCAGCCGGAGGCGGCggtgg                                                >  1:566442/1‑139 (MQ=255)                                                                  aGGCAACCGAACAAAACGACAAGCTCAAGCGGGCGATAATTATTTCAGTAGTGCTGCATGTCATCTTATTTGCGGCGCTGATCTGGAGTTCGTTCGATGAGAat                                                                             >  2:231783/1‑104 (MQ=255)                                                                  aGGCAACCGAACAAAACGACAAGCTCAAGCGGGCGATAATTATTTCAGTAGTGCTGCATGTCATCTTATTTGCGGCGCTGATCTGGAGTTCGTTCGATGAGAat                                                                             <  1:231783/104‑1 (MQ=255)                                                                        ccGAACAAAACGACAAGCTCAAGCGGGCGATAATTATTTCAGTAGTGCTGCATGTCATCTTATTTGCGGCGCTGATCTGGAGTTCGTTCGATGAGAATATAGAAGCTTCAGCCGGAGGCGGCGGTGGTTCGTCCATCGa                                    >  2:391724/1‑139 (MQ=255)                                                                        ccGAACAAAACGACAAGCTCAAGCGGGCGATAATTATTTCAGTAGTGCTGCATGTCATCTTATTTGCGGCGCTGATCTGGAGTTCGTTCGATGAGAATATAGAAGCTTCAGCCGGAGGCGGCGGTGGTTCGTCCATCGa                                    >  2:630086/1‑139 (MQ=255)                                                                          gAACAAAACGACAAGCTCAAGCGGGCGATAATTATTTCAGTAGTGCTGCATGTCATCTTATTTGCGGCGCTGATCTGGAGTTCGTTCGATGAGAATATAGAAGCTTCAGCCGGAGGCGGCGGTGGTTCGTCCATCGACg                                  <  1:818532/139‑1 (MQ=255)                                                                             cAAAACGACAAGCTCAAGCGGGCGATAATTATTTCAGTAGTGCTGCATGTCATCTTATTTGCGGCGCTGATCTGGAGTTCGTTCGATGAGAATATAGAAGCTTCAGCCGGAGGCGGCGGTGGTTCGTCCATCGACGCTg                               >  2:296810/1‑139 (MQ=255)                                                                                aaCGACAAGCTCAAGCGGGCGATAATTATTTCAGTAGTGCTGCATGTCATCTTATTTGCGGCGCt                                                                                                      >  2:725318/1‑65 (MQ=255)                                                                                aaCGACAAGCTCAAGCGGGCGATAATTATTTCAGTAGTGCTGCATGTCATCTTATTTGCGGCGCt                                                                                                      <  1:725318/65‑1 (MQ=255)                                                                                     cAAGCTCAAGCGGGCGATAATTATTTCAGTAGTGCTGCATGTCATCTTATTTGCGGCGCTGATCTGGAGTTCGTTCGATGAGAATATAGAAGCTTCAGCCGGAGGCGGCGGTGGTTCGTCCATCGACGCTGTCATGGtt                       >  2:54765/1‑139 (MQ=255)                                                                                             aGCGGGCGATAATTATTTCAGTAGTGCTGCATGTCATCTTATTTGCGGCGCTGATCTGGAGTTCGTTCGATGAGAATATAGAAGCTTCAGCCGGAGGCGGCGGTGGTTCGTCCATCGACGCTGTCATGGTTGATTCAgg               >  2:364607/1‑139 (MQ=255)                                                                                                  gCGATAATTATTTCAGTAGTGCTGCATGTCATCTTATTTGCGGCGCTGATCTGGAGTTCGTTCGATGAGAATATAGAAGCTTCAGCCGGAGGCGGCGGTGGTTCGTCCATCGACGCTGTCATGGTTGATTCAGGTGCgg          <  1:54765/139‑1 (MQ=255)                                                                                                  gCGATAATTATTTCAGTAGTGCTGCATGTCATCTTATTTGCGGCGCTGATCTGGAGTTCGTTCGATGAGAATATAGAAGCTTCAGCCGGAGGCGGCGGTGGTTCGTCCATCGACGCTGTCATGGTTGATTCAGGTGCgg          <  1:296810/139‑1 (MQ=255)                                                                                                   cGATAATTATTTCAGTAGTGCTGCATGTCATCTTATTTGCGGCGCTGATCTGGAGTTCGTTCGATGAGAATATAGAAGCTTCAGCCGGAGGCGGCGGTGGTTCGTCCATCGACGCTGTCATGGTTGATTCAGGTGCGGt         >  1:749726/1‑139 (MQ=255)                                                                                                          tattTCAGTAGTGCTGCATGTCATCTTATTTGCGGCGCTGATCTGGAGTTCGTTCGATGAGAATATAGAAGCTTCAGCCGGAGGCGGCGGTGGTTCGTCCATCGACGCTGTCATGGTTGATTCAGGTGCGGTAGTTGAg  <  2:217948/139‑1 (MQ=255)                                                                                                          tattTCAGTAGTGCTGCATGTCATCTTATTTGCGGCGCTGATCTGGAGTTCGTTCGATGAGAATATAGAAGCTTCAGCCGGAGGCGGCGGTGGTTCGTCCATCGACGCTGTCATGGTTGATTCAGGTGCGGTAGTTGAg  <  2:749726/139‑1 (MQ=255)                                                                                                          agtttCAGTAGTGCTGCATGTCATCTTATTTGCGGCGCTGATCTGGAGTTCGTTCGATGAGAATATAGAAGCTTCAGCCGGAGGCGGCGGTGGTTCGTCCATCGACGCTGTCATGGTTGATTCAGGTGCGGTAGTTGAg  <  1:45320/137‑1 (MQ=255)                                                                                                            tttCAGTAGTGCTGCATGTCATCTTATTTGCGGCGCTGATCTGGAGTTCGTTCGAt                                                                                   >  1:77269/1‑56 (MQ=255)                                                                                                            tttCAGTAGTGCTGCATGTCATCTTATTTGCGGCGCTGATCTGGAGTTCGTTCGAt                                                                                   <  2:77269/56‑1 (MQ=255)                                                                                                                  |                                                                                                                                   GCGTTTCCCTTGCTTGAAAGAGAGCGGGTAACAGGCGAACAGTTTTTGGAAACCGAGAGTGTCAAAGGCAACCGAACAAAACGACAAGCTCAAGCGGGCGATAATTATTTCAGCAGTGCTGCATGTCATCTTATTTGCGGCGCTGATCTGGAGTTCGTTCGATGAGAATATAGAAGCTTCAGCCGGAGGCGGCGGTGGTTCGTCCATCGACGCTGTCATGGTTGATTCAGGTGCGGTAGTTGAG  >  NC_000913/776284‑776527

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑