Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 872,801 G→A *304* (TAG→TAA)  gsiD → glutathione ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913872,8010GA100.0% 78.8 / NA 26*304* (TAG→TAA) gsiDglutathione ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (12/14);  total (12/14)

TGAGGCTCGAGCGGATATGGTTATCGCGCCGCATGTCGCTGTTTTTCCGGCCCTGGCTATTTTTCTGACCGTACTGGCGTTCAATTTGTTGGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAGTTACGTTTGAATATTGCTTGAAAGGGTAATCACCTCACAGGAAATTATTGCCCTAAGCAAGTGTTGTAACTTTCTGCTGATTTTGTAGAATCGGGTAATTTGGTTAAAAAGCCGCAGCAAGGGACAATTTTTGCA  >  NC_000913/872666‑872936
                                                                                                                                       |                                                                                                                                       
tGATGCTCGAGCGGATATGGTTATCGCGCCGCATGTCGCTGTTTTTCCGGCCCTGGCTATTTTTCTGACCGTACTGGCGTTCAATTTGTTGGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAATTa                                                                                                                                      <  1:309678/139‑1 (MQ=255)
tGAGGCTCGAGCGGATATGGTCATCGCGCCGCATGTCGCTGTTTTTCCGGCCCTGGCTATTTTTCTGACCGTACTGGCGTTCAATTTGTTGGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAATTa                                                                                                                                      <  1:492564/139‑1 (MQ=255)
    gCTCGAGCGGATATGGTTATCGCGCCGCATGTCGCTGTTTTTCCGGCCCTGGCTATTTTTCTGACCGTACTGGCGTTCAATTTGTTGGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAATTACGtt                                                                                                                                  >  1:822373/1‑139 (MQ=255)
          gCGGATATGGTTATCGCGCCGCATGTCGCTGTTTTTCCGGCCCTGGCTATTTTTCTGACCGTACTGGCGTTCAATTTGTTGGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAATTACGTTTGAata                                                                                                                            <  2:817012/139‑1 (MQ=255)
              atatGGTTATCGCGCCGCATGTCGCTGTTTTTCCGGCCCTGGCTATTTTTCTGACCGTACTGGCGTTCAATTTGTTGGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAATTACGTTTGAATATTGc                                                                                                                        <  2:9161/139‑1 (MQ=255)
                       tCGCGCCGCATGTCGCTGTTTTTCCGGCCCTGGCTATTTTTCTGACCGTACTGGCGTTCAATTTGTTGGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAATTa                                                                                                                                      <  2:761921/116‑1 (MQ=255)
                       tCGCGCCGCATGTCGCTGTTTTTCCGGCCCTGGCTATTTTTCTGACCGTACTGGCGTTCAATTTGTTGGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAATTa                                                                                                                                      >  1:761921/1‑116 (MQ=255)
                        cgcgCCGCATGTCGCTGTTTTTCCGGCCCTGGCTATTTTTCTGACCGTACTGGCGTTCAATTTGTTGGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAATTACGTTTGAATATTGCTTGAAAGGGt                                                                                                              >  1:110775/1‑139 (MQ=255)
                        cgcgCCGCATGTCGCTGTTTTTCCGGCCCTGGCTATTTTTCTGACCGTACTGGCGTTCAATTTGTTGGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAATTACGTTTGAATATTGCTTGAAAGGGt                                                                                                              >  2:838756/1‑139 (MQ=255)
                            ccgcATGTCGCTGTTTTTCCGGCCCTGGCTATTTTTCTGACCGTACTGGCGTTCAATTTGTTGGGCGATGGTTTACGCGATGCGCTCGATCCGAAAATTAAAGGATAATTACGTTTGAATATTGCTTGAAAGGGTAATc                                                                                                          >  1:555650/1‑139 (MQ=255)
                              gcATGTCGCTGTTTTTCCGGCCCTGGCTATTTTTCTGACCGTACTGGCGTTCAATTTGTTGGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATGATTACGTTTGAATATTGCTTGAAAGGGTAATCAc                                                                                                        <  1:194185/139‑1 (MQ=255)
                                       ctgttttCCGGCCCTGGCTATTTTTCTGACCGTACTGGCGTTCAATTTGTTGGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAATTACGTTTGAATATTGCTTGAAAGGGTAATCACCTCACAGGa                                                                                               <  1:822238/136‑1 (MQ=255)
                                                 gCCCTGGCTATTTTTCTGACCGTACTGGCGTTCAATTTGTTGGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAATTACGTTTGAATATTGCTTGAAAGGGTAATCACCTCACAGGAAATTATTGcc                                                                                     <  1:699765/139‑1 (MQ=255)
                                                            ttttCTGACCGTACTGGCGTTCAATTTGTTGGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAATTACGTTTGAATATTGCTTGAAAGGGTAATCACCTCACAGGAAATTATTGCCCTAAGCAAgtg                                                                          <  1:552933/139‑1 (MQ=255)
                                                                 tGACCGTACTGGCGTTCAATTTGTTGGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAATTACGTTTGAATATTGCTTGAAAGGGTAATCACCTCACAGGAAATTATTGCCCTAAGc                                                                               >  2:420103/1‑129 (MQ=255)
                                                                 tGACCGTACTGGCGTTCAATTTGTTGGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAATTACGTTTGAATATTGCTTGAAAGGGTAATCACCTCACAGGAAATTATTGCCCTAAGc                                                                               <  1:420103/129‑1 (MQ=255)
                                                                   aCCGTACTGGCGTTCAATTTGTTGGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAATTACGTTTGAATATTGCTTGAAAGGGTAATCACCTCACAGGAAATTATTGCCCTAAGCaa                                                                             <  1:416907/129‑1 (MQ=255)
                                                                   aCCGTACTGGCGTTCAATTTGTTGGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAATTACGGTTGAATATTGCTTGAAAGGGTAATCACCTGACAGCAAATTATTGCCCTAAGCaa                                                                             >  2:416907/1‑129 (MQ=255)
                                                                                 cAATTTGTTGGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAATTACGTTTGAata                                                                                                                            >  2:40489/1‑68 (MQ=255)
                                                                                 cAATTTGTTGGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAATTACGTTTGAata                                                                                                                            <  1:40489/68‑1 (MQ=255)
                                                                                 cAATTTGTTGGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAATTACGTTTGAATATTGCTTGAAAGGGTAATCACCTCACAGGAAatta                                                                                          >  1:669436/1‑102 (MQ=255)
                                                                                 cAATTTGTTGGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAATTACGTTTGAATATTGCTTGAAAGGGTAATCACCTCACAGGAAatta                                                                                          <  2:669436/102‑1 (MQ=255)
                                                                                            gCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAATTACGTTTGAATATTGCTTGAAAGGGTAATCACCTCACAGGAAATTATTGCCCTAAGCAAGTGTTGTAACTTTCTGCTGATTTTGTAGAATCggg                                          >  2:722585/1‑139 (MQ=255)
                                                                                                       cgcgATGCGCTGGATCCGAAAATTAAAGGATAATTACGTTTGAATATTGCTTGAAAGGGTAATCACCTCACAGGAAATTATTGCCCTAAGCAAGTGTTGTAACTTTCTGCTGATTTTGTAGAATCGGGTAATTTGGTTa                               >  1:465969/1‑139 (MQ=255)
                                                                                                                                aaGGATAATTACGTTTGAATATTGCTTGAAAGGGTAATCACCTCACAGGTAATTATTGCCCTAAGCAGGTGTTGTAACTTTCCGCTGTTTTTGTAGAAACGGGTAATTTGGTTAAAAAGTCGCAGCAAGGGACAAtttt      >  2:476206/1‑139 (MQ=255)
                                                                                                                                    aTAATTACGTTTGAATATTGCTTGAAAGGGTAATCACCTCACAGGAAATTATTGCCCTAAGCAAGTGTTGTAACTTTCTGCTGATTTTGTAGAATCGGGTAATTTGGTTAAAAAGCCGCAGCAAGGGACAATTTTTGCa  <  2:822373/139‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                       
TGAGGCTCGAGCGGATATGGTTATCGCGCCGCATGTCGCTGTTTTTCCGGCCCTGGCTATTTTTCTGACCGTACTGGCGTTCAATTTGTTGGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAGTTACGTTTGAATATTGCTTGAAAGGGTAATCACCTCACAGGAAATTATTGCCCTAAGCAAGTGTTGTAACTTTCTGCTGATTTTGTAGAATCGGGTAATTTGGTTAAAAAGCCGCAGCAAGGGACAATTTTTGCA  >  NC_000913/872666‑872936

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑