Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,068,254 G→A *58* (TAG→TAA ymdF → KGG family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,068,2540GA100.0% 97.8 / NA 31*58* (TAG→TAAymdFKGG family protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (16/15);  total (16/15)

CGCGAAAGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGATGCTTATCAGGCCTACCAGAA  >  NC_000913/1068123‑1068387
                                                                                                                                   |                                                                                                                                     
cgcgAAAGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCt                                                                                                                                >  2:424689/1‑139 (MQ=255)
                 aGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAAtcat                                                                                                               >  2:166831/1‑136 (MQ=255)
                                tGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCg                                                                                              >  1:668499/1‑139 (MQ=255)
                                            cGGGGGTAATTTCAAGAATGGCCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTgc                                                                                                        <  2:790117/118‑2 (MQ=255)
                                            cGGGGGTAATTTCAAGAATGGCCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTgc                                                                                                        >  1:790117/1‑117 (MQ=255)
                                                 gTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCAccg                                                                             >  2:310043/1‑139 (MQ=255)
                                                                      aGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATa                                                        <  2:668499/139‑1 (MQ=255)
                                                                      aGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATa                                                        <  1:310043/139‑1 (MQ=255)
                                                                           gcATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTTACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCg                                                                  >  1:256491/1‑124 (MQ=255)
                                                                           gcATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTTACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCg                                                                  <  2:256491/124‑1 (MQ=255)
                                                                                       ggTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCg                                                                  >  1:824377/1‑112 (MQ=255)
                                                                                       ggTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCg                                                                  <  2:824377/112‑1 (MQ=255)
                                                                                       ggTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAg                                       >  1:838382/1‑139 (MQ=255)
                                                                                         tAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCt                                     >  2:774605/1‑139 (MQ=255)
                                                                                                    gtAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATgcg                          <  2:702585/139‑1 (MQ=255)
                                                                                                    gtAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATgcg                          <  1:774605/139‑1 (MQ=255)
                                                                                                    gtAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATgcg                          <  2:345620/139‑1 (MQ=255)
                                                                                                              tCACGGCAAAAGCGACAACTAACCGGGCTAATCAATg                                                                                                                        >  1:382428/1‑37 (MQ=255)
                                                                                                              tCACGGCAAAAGCGACAACTAACCGGGCTAATCAATg                                                                                                                        <  2:382428/37‑1 (MQ=255)
                                                                                                              tCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCaaaaa                                                                                       >  1:127207/1‑68 (MQ=255)
                                                                                                              tCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCaaaaa                                                                                       <  2:127207/68‑1 (MQ=255)
                                                                                                              tCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGATGCTTAt                >  2:486776/1‑139 (MQ=255)
                                                                                                              tCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGATGCTTAt                >  2:624302/1‑139 (MQ=255)
                                                                                                                   gCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGATGCTTATCAGGc           >  2:832139/1‑139 (MQ=255)
                                                                                                                   gCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGATGCTTATCAGGc           <  1:486776/139‑1 (MQ=255)
                                                                                                                       aaGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGATGCTTATCAGGCCTAc       <  1:832139/139‑1 (MQ=255)
                                                                                                                        aGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCggtggt                                                             <  2:755364/84‑1 (MQ=255)
                                                                                                                        aGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCggtggt                                                             >  1:755364/1‑84 (MQ=255)
                                                                                                                            aCAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCAccgc                                                                            >  2:151490/1‑65 (MQ=255)
                                                                                                                            aCAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCAccgc                                                                            <  1:151490/65‑1 (MQ=255)
                                                                                                                            aCAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGATGCTTATCAGGCCTACCagaa  <  2:611873/139‑1 (MQ=255)
                                                                                                                                   |                                                                                                                                     
CGCGAAAGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGATGCTTATCAGGCCTACCAGAA  >  NC_000913/1068123‑1068387

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: