Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,125,011 A→G C106R (TGC→CGC)  mdtH ← multidrug resistance efflux transporter conferring overexpression resistance to norfloxacin and enoxacin

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,125,0110AG100.0% 105.8 / NA 30C106R (TGC→CGC) mdtHmultidrug resistance efflux transporter conferring overexpression resistance to norfloxacin and enoxacin
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (17/13);  total (17/13)

AATGACCGCACCGGCACTGTCCTGCATCATCAACAGCGAGAAAAAACGACCACGCTGCTGTGGACGGATTAATTTCACCACCAGCGCCGAACGCGGCGGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCATGAAAACCACAATAGCCACGGTTCGTGGGCGATACCCATTGTGGCGAATCCGGCGGCGCGCATCAGCATACCGGTAACAATCATCGGTTTGGCACCAAAGCGGTCGGCAATTGCACCGCCGAAAATACCCAGACC  >  NC_000913/1124877‑1125146
                                                                                                                                      |                                                                                                                                       
aaTGACCGCACCGGCACTGTCCTGCATCAGCAACAGCGAGAAAAAACGACCACGCTGCTGTGGACGGATTAATTTCACCACCAGCGTCGAACGCGGCGGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCGTGaa                                                                                                                                     <  1:567297/139‑1 (MQ=255)
                   tCCTGCATCATCAACAGCGAGAAAAAACGACCACGCTGCTGTGGACGGATTAATTTCACCACCAGCGCCGAACGCGGCGGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCGTGAAAACCACAATAGCCa                                                                                                                       <  1:12579/134‑1 (MQ=255)
                   tCCTGCATCATCAACAGCGAGAAAAAACGACCACGCTGCTGTGGACGGATTAATTTCACCACCAGCGCCGAACGCGGCGGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCGTGAAAACCACAATAGCCa                                                                                                                       >  2:12579/1‑134 (MQ=255)
                   tCCTGCATCATCAACAGCGAGAAAAAACGACCACGCTGCTGTGGACGGATTAATTTCACCACCAGCGCCGAACGCGGCGGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCGTGAAAACCACAATAGCCACGGtt                                                                                                                  >  2:462587/1‑139 (MQ=255)
                             tcaACAGCGAGAAAAAACGACCACGCTGCTGTGGACGGATTAATTTCACCACCAGCGCCGAACGCGGCGGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCGTGAAAACCACAATa                                                                                                                           <  1:165581/120‑1 (MQ=255)
                             tcaACAGCGAGAAAAAACGACCACGCTGCTGTGGACGGATTAATTTCACCACCAGCGCCGAACGCGGCGGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCGTGAAAACCACAATa                                                                                                                           >  2:165581/1‑120 (MQ=255)
                             tcaACAGCGAGAAAAAACGACCACGCTGCTGTGGACGGATTAATTTCACCACCAGCGCCGAACGCGGCGGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCGTGAAAACCACAATAGCCACGGTTCGTGGGCGAt                                                                                                        >  2:252119/1‑139 (MQ=255)
                             tcaACAGCGAGAAAAAACGACCACGCTGCTGTGGACGGATTAATTTCACCACCAGCGCCGAACGCGGCGGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCGTGAAAACCACAATAGCCACGGTTCGTGGGCGAt                                                                                                        >  2:524291/1‑139 (MQ=255)
                             tcaACAGCGAGAAAAAACGACCACGCTGCTGTGGACGGATTAATTTCACCACCAGCGCCGAACGCGGCGGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCGTGAAAACCACAATAGCCACGGTTCGTGGGCGAt                                                                                                        >  2:591889/1‑139 (MQ=255)
                             tcaACAGCGAGAAAAAACGACCACCCTGCTGTGGACGGATTAATTTCACCACCAGCGCCGAACGCGGCGGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCGTGAAACCCACAATAGCCACGGTTCGTGGGCGAt                                                                                                        >  2:654467/1‑139 (MQ=255)
                                                         ctgTGGACGGATTAATTTCACCACCAGCGCCGAACGCGGCGGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCGTGAAAACCACAATAGCCACGGTTCGTGGGCGATACCCATTATGGCGAATCCGGCGgcgcgc                                                                            <  1:252119/139‑1 (MQ=255)
                                                                 ggATTAATTTCACCACCAGCGCCGAACGCGGCGGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCGTGAAAACCACAATAGCCACGGTTCGTGGGCg                                                                                                          <  1:50753/101‑1 (MQ=255)
                                                                 ggATTAATTTCACCACCAGCGCCGAACGCGGCGGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCGTGAAAACCACAATAGCCACGGTTCGTGGGCg                                                                                                          >  2:50753/1‑101 (MQ=255)
                                                                 ggATTAATTTCACCACCAGCGCCGAACGCGGCGGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCGTGAAAACCACAATAGCCACGGTTCGTGGGCGATACCCATTGTGGCGAATCCGGCGGCGCGCATCAGCAt                                                                    <  1:591889/139‑1 (MQ=255)
                                                                   aTTAATTTCACCACCAGCGCCGAACGCGGCGGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCGTGAAAACCACAATAGCCACGGTTCGTGGGCGATACCCAtt                                                                                                 >  1:198578/1‑108 (MQ=255)
                                                                   aTTAATTTCACCACCAGCGCCGAACGCGGCGGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCGTGAAAACCACAATAGCCACGGTTCGTGGGCGATACCCAtt                                                                                                 <  2:198578/108‑1 (MQ=255)
                                                                   aTTAATTTCACCACCAGCGCCGAACGCGGCGGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCGTGAAAACCACAATAGCCACGGTTCGTGGGCGATACCCATTGTGGCGAATCCGgcggcg                                                                               <  1:767575/126‑1 (MQ=255)
                                                                   aTTAATTTCACCACCAGCGCCGAACGCGGCGGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCGTGAAAACCACAATAGCCACGGTTCGTGGGCGATACCCATTGTGGCGAATCCGgcggcg                                                                               >  2:767575/1‑126 (MQ=255)
                                                                                   gcgcCGAACGCGGCGGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCGTGAAAAc                                                                                                                                  >  1:71472/1‑59 (MQ=255)
                                                                                   gcgcCGAACGCGGCGGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCGTGAAAAc                                                                                                                                  <  2:71472/59‑1 (MQ=255)
                                                                                   gcgcCGAACGCGGCGGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCGTGAAAACCACAATAGCCa                                                                                                                       >  2:17861/1‑70 (MQ=255)
                                                                                   gcgcCGAACGCGGCGGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCGTGAAAACCACAATAGCCa                                                                                                                       <  1:17861/70‑1 (MQ=255)
                                                                                     gcCGAACGCGGCGGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCGTGAAAACCACAATAGCCACGGTTCGTGGGCGATACCCATTGTGGCGAATCCGGCGGCGCGCATCAGCATACCGGTAACAATCATCGGtt                                                <  1:319406/139‑1 (MQ=255)
                                                                                               gcggATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCGTGaaaa                                                                                                                                   <  2:10149/46‑1 (MQ=255)
                                                                                               gcggATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCGTGaaaa                                                                                                                                   >  1:10149/1‑46 (MQ=255)
                                                                                                        acaacGTGCCACCGAGTCCCGAGAGCAGGCGTGAAAACCACAATAGCCACGGTTCGTGGGCGATACCCATTGTGGCGAATCCGGCGGCGCGCATCAGCATACCGGTAACAATCATCGGTTTGGCACCAAAGCGGTCGGc                             >  1:114105/1‑139 (MQ=255)
                                                                                                             gTGCCACCGAGTCCCGAGAGCAGGCGTGAAAACCACAATAGCCACGGTTCGTGGGCGATACCCATTGCGGCGAATCCGGCGGCGCGCATCAGCATACCGGTAACAATCATCGGTTTGGCACCAAAGCGGTCGGCAATTg                        >  1:635030/1‑139 (MQ=255)
                                                                                                                ccACCGAGTCCCGAGAGCAGGCGTGAAAACCACAATAGCCACGGTTCGTGGGCGATACCCATTGTGGCGAATCCGGCGGCGCGCATCAGCATACCGGTAACAATCATCGGTTTGGCACCAAAGCGGTCGGCAATTGCAc                     >  2:343827/1‑139 (MQ=255)
                                                                                                                                 cAGGCGTGAAAACCACAATAGCCACGGTTCGTGGGCGATACCCATTGTGGCGAATCCGGCGGCGCGCATCAGCATACCGGTAACAATCATCGGTTTGGCACCAAAGCGGTCGGCAATTGCACCGCCGAAAATACCCAGa    >  1:222642/1‑139 (MQ=255)
                                                                                                                                   ggCGTGAAAACCACAATAGCCACGGTTCGTGGGCGATACCCATTGTGGCGAATCCGGCGGCGCGCATCAGCATACCGGTAACAATCATCGGTTTGGCACCAAAGCGGTCGGCAATTGCACCGCCGAAAATACCCAGAcc  <  2:114105/139‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                       
AATGACCGCACCGGCACTGTCCTGCATCATCAACAGCGAGAAAAAACGACCACGCTGCTGTGGACGGATTAATTTCACCACCAGCGCCGAACGCGGCGGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCATGAAAACCACAATAGCCACGGTTCGTGGGCGATACCCATTGTGGCGAATCCGGCGGCGCGCATCAGCATACCGGTAACAATCATCGGTTTGGCACCAAAGCGGTCGGCAATTGCACCGCCGAAAATACCCAGACC  >  NC_000913/1124877‑1125146

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: