Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,148,691	G→A	*357* (TAG→TAA)	plsX →	putative phosphate acyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,148,691	0	G	A	100.0%	45.7 / NA	16	*357* (TAG→TAA)	plsX	putative phosphate acyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (10/6);  total (10/6)

GCAGTGCAGGCGGTGCAGCGACAAGTTCCTCAGCGAATTGCCGCTCGCCTGGAATCTGTATACCCAGCTGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGACAAACGC  >  NC_000913/1148578‑1148817                                                                                                                  |                                                                                                                               gCAGTGCAGGCGGTGCAGCGACAAGTTCCTCAGCGAATTGCCGCTCGCCTGGAATCTGTATACCCAGCTGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAACAGGACGCTGCCAg                                                                                                                  <  1:614303/128‑1 (MQ=255) gCAGTGCAGGCGGTGCAGCGACAAGTTCCTCAGCGAATTGCCGCTCGCCTGGAATCTGTATACCCAGCTGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAACAGGACGCTGCCAg                                                                                                                  >  2:614303/1‑128 (MQ=255)   aGTGCAGGCGGTGCAGCGACAAGTTCCTCAGCGAATTGCCGCTCGCCTGGAATCTGTATACCCAGCTGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAACAGGACGCTGCCAGCGAACTCGCAGtt                                                                                                     >  2:135980/1‑139 (MQ=255)       cAGGCGGTGCAGCGACAAGTTCCTCAGCGAATTGCCGCTCGCCTGGAATCTGTATACCCAGCTGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAACAGGACGCTGCCAGCGAACTCGCAGTTTGCa                                                                                                 >  1:169626/1‑139 (MQ=255)                                           gCTCGCCTGGAATCTGTATACCCAGCTGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAACAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCGTAc                                                             <  1:135980/139‑1 (MQ=255)                                                                   gCTGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAACAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCg                                                                               <  1:453490/97‑1 (MQ=255)                                                                   gCTGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAACAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCg                                                                               >  2:453490/1‑97 (MQ=255)                                                                   gCTGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAACAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCGTACATGTATACGAAGATTATTGGTACt                                     >  1:242175/1‑139 (MQ=255)                                                                    cTGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAACAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTg                                                                        <  1:764185/103‑1 (MQ=255)                                                                    cTGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAACAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTg                                                                        >  2:764185/1‑103 (MQ=255)                                                                     tGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAACAGGACGCt                                                                                                                       <  1:796589/55‑1 (MQ=255)                                                                     tGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAACAGGACGCt                                                                                                                       >  2:796589/1‑55 (MQ=255)                                                                            gAGCTGCTGGACTGTGGCAAAAGCGGAACTCTGCGGTAACAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTAt                            >  1:300680/1‑139 (MQ=255)                                                                            gAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAACAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTAt                            <  2:242175/139‑1 (MQ=255)                                                                                               aaaGCGGAACTCTGGGGTAACAGGACGCAGCCAGCGAACTCGCAGTATGCAAGTGACGGTATATAACCGAAAAGTGAATGAGCGTACATGTATACGAAGATGATTGCTACTGACAGATAACTGCACGAACAAGTGCAGa         >  2:534145/1‑139 (MQ=255)                                                                                                      aaCTCTGCGGTAACAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGACAAACGc  >  2:306878/1‑139 (MQ=255)                                                                                                                  |                                                                                                                               GCAGTGCAGGCGGTGCAGCGACAAGTTCCTCAGCGAATTGCCGCTCGCCTGGAATCTGTATACCCAGCTGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGACAAACGC  >  NC_000913/1148578‑1148817

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑