Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	10,647	A→G	V237A (GTC→GCC)	yaaW ←	UPF0174 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	10,647	0	A	G	100.0%	74.6 / NA	22	V237A (GTC→GCC)	yaaW	UPF0174 family protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (9/13);  total (9/13)

CGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGCGCTGCCGCTGACCGCTTTAACCCCATTTAGTGCCGCACCTACAGGGCCTCCCAGCCCCGCGCCG  >  NC_000913/10515‑10781                                                                                                                                     |                                                                                                                                       cGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGGCCTGAg                                                                                                                                  <  1:118956/139‑1 (MQ=255)     tgaatgGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGGCCTGAGTGgc                                                                                                                              <  2:290443/139‑1 (MQ=255)                           gAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGGCCTGAGTGGCGCTAACCATCCGGCgcaggcag                                                                                                        <  2:361391/139‑1 (MQ=255)                                      gtgtTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGGCCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAg                                                                                             >  1:639560/1‑139 (MQ=255)                                       tgtTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGGCCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGt                                                                                            >  1:46407/1‑139 (MQ=255)                                       tgtTTTCAGGGCGCGGCATAATAATCAGACAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCAAGGTTTCCGGTAGCAGGGCTGAGTGGCGGTAACCATCCGGCGCAGGGAGGGGATTTGCAGt                                                                                            <  2:285758/139‑1 (MQ=255)                                              aGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGGCCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTg                                                                                     >  2:810606/1‑139 (MQ=255)                                                   gcgGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGGCCTGAGTGGCGCTAACAATCCGGCCAAGGCAGGGAATTTGCAGTACGGCTGGAATc                                                                                >  1:342693/1‑139 (MQ=255)                                                        ataataATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGGCCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCAc                                                                           <  2:46407/139‑1 (MQ=255)                                                          aataatCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGGCCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCAcgc                                                                         >  2:523940/1‑139 (MQ=255)                                                                 aGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGGCCTGAGTGGCGCTAACCATCCGGCgcaggc                                                                                                          >  1:461593/1‑99 (MQ=255)                                                                 aGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGGCCTGAGTGGCGCTAACCATCCGGCgcaggc                                                                                                          <  2:461593/99‑1 (MQ=255)                                                                 aGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGGCCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGgc                                                                  >  1:550837/1‑139 (MQ=255)                                                                  gCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGGCCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGgcg                                                                 <  2:639560/139‑1 (MQ=255)                                                                       tGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGGCCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGgcg                                                                 <  1:106202/134‑1 (MQ=255)                                                                       tGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGGCCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGgcg                                                                 >  2:106202/1‑134 (MQ=255)                                                                        ggggCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGGCCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGCGCTGCCg                                                           <  2:354496/139‑1 (MQ=255)                                                                                  cTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGGTACAGGCCTGAGGGGCCGTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGCGCTGCCGCTGACCGCtt                                                 <  2:342693/139‑1 (MQ=255)                                                                                                  ggAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGGCCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGCGCTGCCGCTGACCGCTTTAACCCCATTTAGTGc                                 <  1:810606/139‑1 (MQ=255)                                                                                                  ggAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGGCCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGCGCTGCCGCTGACCGCTTTAACCCCATTTAGTGc                                 <  1:703267/139‑1 (MQ=255)                                                                                                      aatgaaaatTTTCCCCGGTTTCCGGTATCAGGCCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGCGCTGCCGCTGACCGCTTTAACCCCATTTAGTGCCGCa                             >  2:118446/1‑139 (MQ=255)                                                                                                                                 tCAGGCCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGCGCTGCCGCTGACCGCTTTAACCCCATTTAGTGCCGCACCTACAGGGCCTCCCAGCCccgcgccg  <  1:118446/139‑1 (MQ=255)                                                                                                                                     |                                                                                                                                       CGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGCGCTGCCGCTGACCGCTTTAACCCCATTTAGTGCCGCACCTACAGGGCCTCCCAGCCCCGCGCCG  >  NC_000913/10515‑10781

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑