Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,245,600	G→A	*147* (TAG→TAA)	ycgY →	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,245,600	0	G	A	100.0%	52.2 / NA	18	*147* (TAG→TAA)	ycgY	uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (8/10);  total (8/10)

GTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAGAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGTTAAGGTGTGGGTTGTGCCTCTTTGGTTGAGGGTTGCGTCGTTGCTGACTTA  >  NC_000913/1245473‑1245730                                                                                                                                |                                                                                                                                   gTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATa                                                                                                                         >  1:197281/1‑139 (MQ=255)  tCGGCGTAAAACGGCTGGTGAAGAAAGTGAAACACTTACCCGGAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATAg                                                                                                                        <  2:197281/139‑1 (MQ=255)    ggCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCt                                                                                                                      <  2:745195/139‑1 (MQ=255)              gCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCTATGGTGTTAt                                                                                                            <  1:623929/139‑1 (MQ=255)                                       aCCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCTATgg                                                                                                                  >  1:682954/1‑108 (MQ=255)                                       aCCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCTATgg                                                                                                                  <  2:682954/108‑1 (MQ=255)                                                      gCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCAt                                                                                            >  1:229568/1‑115 (MQ=255)                                                      gCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCAt                                                                                            <  2:229568/115‑1 (MQ=255)                                                      gCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTc                                                                    >  2:381175/1‑139 (MQ=255)                                                               ggCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAgc                                                           <  1:381175/139‑1 (MQ=255)                                                                gCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAgcg                                                          <  2:839279/139‑1 (MQ=255)                                                                       cGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGTTa                                                   <  2:188789/139‑1 (MQ=255)                                                                         tATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCTATGGTGTTATCTGCCGACAACg                                                                                                >  2:648027/1‑92 (MQ=255)                                                                         tATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCTATGGTGTTATCTGCCGACAACg                                                                                                <  1:648027/92‑1 (MQ=255)                                                                              cAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGTTAAGGtgtg                                            >  2:773949/1‑139 (MQ=255)                                                                                   aTTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGTTAAGGTGTGGGTtg                                       <  1:773949/139‑1 (MQ=255)                                                                                        cgcAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGTTAAGGTGTGGGTTGTGCct                                  >  1:135091/1‑139 (MQ=255)                                                                                                                        tCTGTGTAAAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGTTAAGGTGTGGGTTGTGCCTCTTTGGTTGAGGGTTGCGTCGTTGCTGACTTa  >  1:813835/1‑139 (MQ=255)                                                                                                                                |                                                                                                                                   GTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAGAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGTTAAGGTGTGGGTTGTGCCTCTTTGGTTGAGGGTTGCGTCGTTGCTGACTTA  >  NC_000913/1245473‑1245730

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑