Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,457,801	A→G	Y102C (TAC→TGC)	paaF →	2,3‑dehydroadipyl‑CoA hydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,457,801	0	A	G	100.0%	64.8 / NA	19	Y102C (TAC→TGC)	paaF	2,3‑dehydroadipyl‑CoA hydratase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (6/13);  total (6/13)

CCGCTGGGGCCGATCTCAACGAAATGGCAGAAAAAGATCTCGCGGCCACCTTAAACGATACACGTCCGCAGCTATGGGCGCGATTGCAGGCCTTTAATAAACCACTTATCGCAGCCGTCAATGGTTACGCGCTTGGGGCGGGTTGCGAACTGGCATTGTTGTGCGATGTGGTGGTTGCCGGAGAGAACGCGCGTTTTGGGTTGCCGGAAATCACTCTCGGCATCATGCCTGGCGCAGGCGGAACGCAACGTTTA  >  NC_000913/1457675‑1457928                                                                                                                               |                                                                                                                                ccGCTGGGGCCGATCTCAACGAAATGGCAGAAAAAGATCTCGCGGCCACCTTAAACGATACACGTCCGCAGCTATGGGCGCGATTGCAGGCCTTTAATAAACCACTTATCGCAGCCGTCAATGGTTGCGCGTTTGGGGc                                                                                                                     <  2:548795/139‑1 (MQ=255)         gCCGATCTCAACGAAATGGCAGAAAAAGATCTCGCGGCCACCTTAAACGATACACGTCCGCAGCTATGGGCGCGATTGCAGGCCTTTAATAAACCACTTATCGCAGCCGTCAATGGTTGCGCGCTTGGGGCGGGTTGCg                                                                                                             <  2:657989/139‑1 (MQ=255)              tctcAACGAAATGGCAGAAAAAGATCTCGCGGCCACCTTAAACGATACACGTCCGCAGCTATGGGCGCGATTGCAGGCCTTTAATAAACCACTTATCGCAGCCGTCAATGGTTGCGCGCTTGGGGCGGGTTGCGAACTg                                                                                                        >  2:127/1‑139 (MQ=255)                           gCAGAAAAAGATCTCGCGGCCACCTTAAACGATACACGTCCGCAGCTATGGGCGCGATTGCAGGCCTTTAATAAACCACTTATCGCAGCCGTCAATGGTTGCGCGCTTGGGGCGGGTTGCGAACTGGCATTGTTGTGCg                                                                                           <  1:388827/139‑1 (MQ=255)                                               cACCTTAAACGATACACGTCCGCAGCTATGGGCGCGATTGCAGGCCTTTAATAAACCACTTATCGCAGCCGTCAATGGTTGCGCGCTTGGGGCGGGTTGCGAACTGGCATTGTTGTGCGATGTGGTGGTTGCCGgagag                                                                       <  2:443637/139‑1 (MQ=255)                                                                gTCCGCAGCTATGGGCGCGATTGCAGGCCTTTAATAAACCACTTATCGCAGCCGTCAATGGTTGCGCGCTTGGGGCGGGTTGCGAACTGGCATTGTTGTGCGATGTGGTGGTTGCCGGAGAGAACGCGCGTTTTGGGtt                                                      >  1:470473/1‑139 (MQ=255)                                                                 tCCGCAGCTATGGGCGCGATTGCAGGCCTTTAATAAACCACTTATCGCAGCCGTCAATGGTTGCGCGCTTGGGGCGGGTTGCGAACTGGCa                                                                                                     >  1:133938/1‑91 (MQ=255)                                                                 tCCGCAGCTATGGGCGCGATTGCAGGCCTTTAATAAACCACTTATCGCAGCCGTCAATGGTTGCGCGCTTGGGGCGGGTTGCGAACTGGCa                                                                                                     <  2:133938/91‑1 (MQ=255)                                                                        cTATGGGCGCGATTGCAGGCCTTTAATAAACCACTTATCGCAGCCGTCAATGGTTGCGCGCTTGGGGc                                                                                                                     <  1:302406/68‑1 (MQ=255)                                                                        cTATGGGCGCGATTGCAGGCCTTTAATAAACCACTTATCGCAGCCGTCAATGGTTGCGCGCTTGGGGc                                                                                                                     >  2:302406/1‑68 (MQ=255)                                                                         tATGGGCGCGATTGCAGGCCTTTAATAAACCACTTATCGCAGCCGTCAATGGTTGCGCGCTTGGGGCGGGTTGCGAACTGGCATTGTTGTGCGATGTGGTGGTTGCCGGAGAGAACGCGCGTTTTGGGTTGCCGGAAAt                                             <  1:127/139‑1 (MQ=255)                                                                         tATGGGCGCGAGTGCAGGCCTTTAATAAACCACTTATCGCAGCCGTCAATGGTTGCGCGCTTGGGGCGGGTTGCGAACTGGCATTGTTGTGCGATGTGGTGGTTGCCGGAGTGAACGCGCGTTTTGGGTTGCCGGAAAt                                             <  1:543127/139‑1 (MQ=255)                                                                                                    aaCCACTTATCGCAGCCGTCAATGGTTGCGCGCTTGGGGCGGGTTGCGAACTGGCATTGTTGTGCGATGTGGTGGTTGCCGGAGAGAACGCGCGTTTTGGGTTGCCGGAAATCACTCTCGGCATCATGCCTGGCGCAgg                  <  1:688314/139‑1 (MQ=255)                                                                                                            aTCGCAGCCGTCAATGGTTGCGCGCTTGGGGCGGGTTGCGAACTGGCATTGTTGTGCGATGTGGTGGTTGCCGga                                                                          <  2:131011/75‑1 (MQ=255)                                                                                                            aTCGCAGCCGTCAATGGTTGCGCGCTTGGGGCGGGTTGCGAACTGGCATTGTTGTGCGATGTGGTGGTTGCCGga                                                                          >  1:131011/1‑75 (MQ=255)                                                                                                             tCGCAGCCGTCAATGGTTGCGCGCTTGGGGCGGGTTGCGAACTGGCATTGTTGTGCGATGTGGTGGTTGCCGGAGAGAACGCGCGTTTTGGGTTGCCGGAAATCACTCTCGGCATCATGCCTGGCGCAGGCGGAACGCa         <  1:216200/139‑1 (MQ=255)                                                                                                                   ccGTCAATGGTTGCGCGCTTGGGGCGGGTTGCGAACTGGCATTGTTGTGCGATGtggtgg                                                                                  <  2:771063/60‑1 (MQ=255)                                                                                                                   ccGTCAATGGTTGCGCGCTTGGGGCGGGTTGCGAACTGGCATTGTTGTGCGATGtggtgg                                                                                  >  1:771063/1‑60 (MQ=255)                                                                                                                    cGTCAATGGTTGCGCGCTTGGGGCGGGTTGCGAACTGGCATTGTTGTGCGATGTGGTGGTTGCCGGAGAGAACGCGCGTTTTGGGTTGCCGGAAATCACTCTCGGCATCATGCCTGGCGCAGGCGGAACGCAACGTTTa  <  2:470473/139‑1 (MQ=255)                                                                                                                               |                                                                                                                                CCGCTGGGGCCGATCTCAACGAAATGGCAGAAAAAGATCTCGCGGCCACCTTAAACGATACACGTCCGCAGCTATGGGCGCGATTGCAGGCCTTTAATAAACCACTTATCGCAGCCGTCAATGGTTACGCGCTTGGGGCGGGTTGCGAACTGGCATTGTTGTGCGATGTGGTGGTTGCCGGAGAGAACGCGCGTTTTGGGTTGCCGGAAATCACTCTCGGCATCATGCCTGGCGCAGGCGGAACGCAACGTTTA  >  NC_000913/1457675‑1457928

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑