Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,665,120	G→A	*205* (TAG→TAA)	dmsD →	twin‑argninine leader‑binding protein for DmsA and TorA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,665,120	0	G	A	100.0%	88.2 / NA	28	*205* (TAG→TAA)	dmsD	twin‑argninine leader‑binding protein for DmsA and TorA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (17/11);  total (17/11)

GTGCACTGGGTGAACTGGCTCGCTTAACGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAGGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGC  >  NC_000913/1665032‑1665252                                                                                         |                                                                                                                                     gTGCACTGGGTGAACTGGCTCGCTTAACGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCGGAt                                                                                                                >  1:235408/1‑111 (MQ=255) gTGCACTGGGTGAACTGGCTCGCTTAACGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCGGAt                                                                                                                <  2:235408/111‑1 (MQ=255) gTGCACTGGGTGAACTGGCTCGCTTAACGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCaata                                                                                    >  1:62980/1‑139 (MQ=255) gTGCACTGGGTGAACTGGCTCGCTTAACGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCaata                                                                                    >  2:111707/1‑139 (MQ=255)         ggTGAACTGGCTCGCTTAACGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTa                                                                            >  1:765054/1‑139 (MQ=255)              aCTGGCTCGCTTAACGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCaa                                                                                      <  2:591169/124‑1 (MQ=255)              aCTGGCTCGCTTAACGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCaa                                                                                      >  1:591169/1‑124 (MQ=255)                   cTCGCTTAACGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCaata                                                                                    <  1:411196/121‑1 (MQ=255)                   cTCGCTTAACGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCaata                                                                                    >  2:411196/1‑121 (MQ=255)                   cTCGCTTAACGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACg                                                                  >  2:50186/1‑139 (MQ=255)                       cTTAACGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCgg                                                                                         <  1:170216/112‑1 (MQ=255)                       cTTAACGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCgg                                                                                         >  2:170216/1‑112 (MQ=255)                           aCGGTGGCGCAATGGCAATCACAACTGTTAATTCCTGTTGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATAGTGCCGGATGGTGACGATTACGCGCAATCCGTCAATAATATGTTACAGTGTCACGTTTTTTTa                                                          <  2:393115/139‑1 (MQ=255)                            cGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTAt                                                         <  1:111707/139‑1 (MQ=255)                            cGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTAt                                                         >  2:288144/1‑139 (MQ=255)                                ggCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTAcg                                                                                                   >  1:79130/1‑93 (MQ=255)                                ggCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTAcg                                                                                                   <  2:79130/93‑1 (MQ=255)                                           aaTCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTa                                                                            <  2:204836/105‑1 (MQ=255)                                           aaTCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTa                                                                            >  1:204836/1‑105 (MQ=255)                                                      ttAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAAt                                                                                             <  2:105303/77‑1 (MQ=255)                                                      ttAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAAt                                                                                             >  1:105303/1‑77 (MQ=255)                                                       tAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCgg                              <  2:765054/139‑1 (MQ=255)                                                           tCCTGTCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTgg                          >  1:502604/1‑139 (MQ=255)                                                              tGTCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCct                       >  2:211271/1‑139 (MQ=255)                                                                tCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGAAATAATAGGTTACAGTGTCACGTTTTTTTATCTCGTAAAGCACGCACTGCTTTTGCGGCTGGCctct                     >  2:497514/1‑139 (MQ=255)                                                                     gTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGc                >  2:513148/1‑139 (MQ=255)                                                                                gTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGt     >  1:43416/1‑139 (MQ=255)                                                                                   tCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGc  <  1:50186/139‑1 (MQ=255)                                                                                         |                                                                                                                                     GTGCACTGGGTGAACTGGCTCGCTTAACGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAGGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGC  >  NC_000913/1665032‑1665252

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑