Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,744,740 C→T G428G (GGC→GGT mdtK → multidrug efflux system transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,744,7400CT100.0% 65.3 / NA 22G428G (GGC→GGTmdtKmultidrug efflux system transporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (12/10);  total (12/10)

CCATTTTCTATATTACCTTTACGGCTTACTGGGTGCTGGGCTTGCCAAGCGGCTATATTCTGGCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCATTATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCAACGAGCATCCCGCTAATAAAGACAAGGCGCAACCTTCACGGGTTGCGCCTGTATTTTTACG  >  NC_000913/1744613‑1744875
                                                                                                                               |                                                                                                                                       
acaatttCTAGATTGCCTTTACGCCTGATTGGGTGCTGGGCTTGCCAAGCGGTTATATTCTGGCACTGACTGGTGTGGTTGTTGAACCTATCGGGCCAGCAGGCTTCTGGATAGGCTTTATTCTTGGTCTGACGTCGGc                                                                                                                              <  2:650104/135‑1 (MQ=255)
                                 tGCTGGGCTTGCCAAGCGGCTATATTCTGGCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGTCTGACGTCGGc                                                                                                                              <  2:791028/106‑1 (MQ=255)
                                 tGCTGGGCTTGCCAAGCGGCTATATTCTGGCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGTCTGACGTCGGc                                                                                                                              >  1:791028/1‑106 (MQ=255)
                                    tGGGCTTGCCAAGCGGCTATATTCTGGCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGTCTGACGTCGGCAg                                                                                                                            <  2:233495/105‑1 (MQ=255)
                                    tGGGCTTGCCAAGCGGCTATATTCTGGCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGTCTGACGTCGGCAg                                                                                                                            >  1:233495/1‑105 (MQ=255)
                                    tGGGCTTGCCAAGCGGCTATATTCTGGCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGTCTGACGTCGGCAGCCATTATGATGATGt                                                                                                             >  1:649971/1‑120 (MQ=255)
                                    tGGGCTTGCCAAGCGGCTATATTCTGGCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGTCTGACGTCGGCAGCCATTATGATGATGt                                                                                                             <  2:649971/120‑1 (MQ=255)
                                           gCCAAGCGGCTATATTCTGGCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGTCTGACGTCGGCAGCCATTATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTg                                                                                   >  1:583625/1‑139 (MQ=255)
                                                gCGGCTATATTCTGGCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGTCTGACATCGGCAGCCATTATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTc                                                                              >  2:743468/1‑139 (MQ=255)
                                                 cGGCTATATTCTGGCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATGGGTCTGACGTCGGCAGCCATTATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCa                                                                             >  1:505066/1‑139 (MQ=255)
                                                                 cTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGTCTGACGTCGGCAGCCATTATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCAAc                                                             >  2:202972/1‑139 (MQ=255)
                                                                   gACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGTCTGACGTCGGCAGc                                                                                                                           <  2:351430/75‑1 (MQ=255)
                                                                   gACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGTCTGACGTCGGCAGc                                                                                                                           >  1:351430/1‑75 (MQ=255)
                                                                       gATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTACTATTGGTCTGACGTCGGCAGCCATTATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCAACGAGcgt                                                       <  2:503931/139‑3 (MQ=255)
                                                                        aTCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATCATTGGTCTGACGTCGGCAGCCATTATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGGCATCATTCTGCAACGAGCATc                                                      <  2:505066/139‑1 (MQ=255)
                                                                            ggTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGTCTGACGTCGGCAGCCATTATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCAACGAGCAt                                                       >  2:373926/1‑134 (MQ=255)
                                                                            ggTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGTCTGACGTCGGCAGCCATTATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCAACGAGCAt                                                       <  1:373926/134‑1 (MQ=255)
                                                                                              gCCAGCAGGCTTCTGGATAGGCTTTATTATTGGTCTGACGTCGGCAGCCATTATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCAACGAGCATCCCGCTAATAAAGACAAGGCGCa                                <  2:583625/139‑1 (MQ=255)
                                                                                                             gATAGGCTTTATTATTGGTCTGACGTCGGCAGCCATTATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCAACGAGCATCCCGCTAATAAAGCCAAGGCGCAACCTTCACGGGTTgc                 >  1:468025/1‑139 (MQ=255)
                                                                                                                    tttATTATTGGTCTGACGTCGGCAGCCATTATGATGATGTTGCGTATGc                                                                                                    <  2:580057/49‑1 (MQ=255)
                                                                                                                    tttATTATTGGTCTGACGTCGGCAGCCATTATGATGATGTTGCGTATGc                                                                                                    >  1:580057/1‑49 (MQ=255)
                                                                                                                            tGGTCTGACGTCGGCAGCCATTATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCAACGAGCATCCCGCTAATAAAGACAAGGCGCAACCTTCACGGGTTGCGCCTGTATTTTTACg  >  1:180354/1‑139 (MQ=255)
                                                                                                                               |                                                                                                                                       
CCATTTTCTATATTACCTTTACGGCTTACTGGGTGCTGGGCTTGCCAAGCGGCTATATTCTGGCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCATTATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCAACGAGCATCCCGCTAATAAAGACAAGGCGCAACCTTCACGGGTTGCGCCTGTATTTTTACG  >  NC_000913/1744613‑1744875

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: