Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,757,721 C→T *335* (TAG→TAA ldtE ← murein L,D‑transpeptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,757,7210CT100.0% 49.0 / NA 17*335* (TAG→TAAldtEmurein L,D‑transpeptidase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (10/7);  total (10/7)

ATGACGCAGCTCGTGCTAACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGCTACTGCGTCACGCGTAACATATTCCCTTGCTCTGGTTCACCATTCTGCGCTGACTCTACTGAAGGCGCATTGCTGGCTGCGGGAG  >  NC_000913/1757599‑1757806
                                                                                                                          |                                                                                     
aTGACGCAGCTCGTGCTAACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTa                                                                       >  2:172082/1‑139 (MQ=255)
    cGCAGCTCGTGCTAACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACat                                                                   >  2:110651/1‑139 (MQ=255)
                       cGTCTGGACAACATGGCTACTAAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTtcaccatt                                                <  1:110651‑M1/139‑9 (MQ=255)
                             gACAACATGGCTACTAAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTtcaccattctgcgc                                          <  1:756250‑M1/139‑15 (MQ=255)
                                                                  tgtgAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTtcaccattctgcgctgactctactgaaggcgcattgctggctgcgggagtt     >  1:124125‑M1/1‑88 (MQ=255)
                                                                   gtgaagtgaaAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGt                                                                        <  1:411264/71‑1 (MQ=255)
                                                                   gtgaagtgaaAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGt                                                                        >  2:411264/1‑71 (MQ=255)
                                                                   gtgaagtgaaAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTtcaccattctgcgctgactctactgaaggcgcattgctggctgcgggagttg    >  1:590247‑M1/1‑87 (MQ=255)
                                                                     gaagtgaaAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCTGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTtcaccattctgcgctgactctactgaaggcgcattgctggctgcgggagttgct  >  2:592836‑M1/1‑85 (MQ=255)
                                                                                     cacaTTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCAcgcg                                                                         >  1:517462/1‑52 (MQ=255)
                                                                                     cacaTTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCAcgcg                                                                         <  2:517462/52‑1 (MQ=255)
                                                                                            tgCGCCATTTTTTTTGTCTGCCGTTTACCGTTACt                                                                                   <  2:781251/35‑1 (MQ=255)
                                                                                            tgCGCCATTTTTTTTGTCTGCCGTTTACCGTTACt                                                                                   >  1:781251/1‑35 (MQ=255)
                                                                                             gcgcCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTtcaccattctgcgctgactctactgaaggcgcattgctggctgcggg         >  2:401092‑M1/1‑61 (MQ=255)
                                                                                             gcgcCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTtcaccattctgcgctgactctactgaaggcgcattgctggctgcggg         <  1:401092‑M1/108‑48 (MQ=255)
                                                                                                                ccGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTtcaccattctgcg                                           >  2:290648‑M1/1‑42 (MQ=255)
                                                                                                                ccGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTtcaccattctgcg                                           <  1:290648‑M1/55‑14 (MQ=255)
                                                                                                                          |                                                                                     
ATGACGCAGCTCGTGCTAACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGCTACTGCGTCACGCGTAACATATTCCCTTGCTCTGGTTCACCATTCTGCGCTGACTCTACTGAAGGCGCATTGCTGGCTGCGGGAG  >  NC_000913/1757599‑1757806

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: