Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,761,149	G→A	H215Y (CAT→TAT)	sufD ←	component of SufBCD Fe‑S cluster assembly scaffold

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,761,149	0	G	A	100.0%	62.0 / NA	21	H215Y (CAT→TAT)	sufD	component of SufBCD Fe‑S cluster assembly scaffold
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (10/11);  total (10/11)

GTAACACTGCGCCACCCAGCAGGAAACTGTGGCTAAATGCGGTGGCATCCTCAGCCAGCAACAAATCGTTATGAGCAAAGTGGTGACTGAGCGGGTTTTCAAACGCCAGCTTGATATGCTGCAAGTGGGCATTCGCTGCGACGTTGATAGTGAACCGTGCCCCGGTAAAATGACGAGCATCATTCAGGCTGACAAAATGTTCGATCACCGTTGCTTCGGCACCTTCCGCCAGAT  >  NC_000913/1761032‑1761265                                                                                                                      |                                                                                                                     gTAACACTGCGCCACCCAGCAGGAAACTGTGGCTAAATGCGGTGGCATCCTCAGCCAGCAACAAATCGTTATGAGCAAAGTGGTGACTGAGCGGGTTTTCAAACGCCAGCTTGATATACTGCAAGTGGGCATTCGCTGc                                                                                                 >  1:434072/1‑139 (MQ=255)      acTGCGCCACCCAGCAGGAAACTGTGGCTAAATGCGGTGGCATCCTCAGCCAGCAACAAATCGTTATGAGCAAAGTGGTGACTGAGCGGGTTTTCAAACGCCAGCTTGATATACTGCAAGTGGGCATTCGCTGCGACGt                                                                                            <  2:367039/139‑1 (MQ=255)      acTGCGCCACCCAGCAGGAAACTGTGGCTAAATGCGGTGGCATCCTCAGCCAGCAACAAATCGTTATGAGCAAAGTGGTGACTGAGCGGGTTTTCAAACGCCAGCTTGATATACTGCAAGTGGGCATTCGCTGCGACGt                                                                                            <  2:358912/139‑1 (MQ=255)           gcCACCCAGCAGGAAACTGTGGCTAAATGCGGTGGCATCCTCAGCCAGCAACTAATCGTTATGAGCAAAGTGGTGACTGAGCGGGTTTTCAAACGCCAGCTTGATATACTGCAAGTGGGCATTCGCTGCGACGTTGATa                                                                                       <  1:554678/139‑1 (MQ=255)               cccAGCAGGAAACTGTGGCTAAATGCGGTGGCATCCTCAGCCAGCAACAAATCGTTATGAGCAAAGTGGTGACTGAGCGGGTTTTCAAACGCCAGCTTGATATACTGCAAGTGGGCATTCGCTGCGACGTTGATAGTGa                                                                                   >  2:148673/1‑139 (MQ=255)                      ggAAACTGTGGCTAAATGCGGTGGCATCCTCAGCCAGCAACAAATCGTTATGAGCAAAGTGGTGACTGAGCGGGTTTTCAAACGCCAGCTTGATATACTGCAAGTGGGCATTCGCTGCGACGTTGATAGTGAACCGTGc                                                                            <  1:148673/139‑1 (MQ=255)                                gCTAAATGCGGTGGCATCCTCAGCCAGCAACAAATCGTTATGAGCAAAGTGGTGACTGAGCGGGTTTTCAAACGCCAGCTTGATATACTGCAAGTGGGCATTCGCTGCGACGTTGATAGTGAACCGTGCCCCGGTaaaa                                                                  >  1:773219/1‑139 (MQ=255)                                          gTGGCATCCTCAGCCAGCAACAAATCGTTATGAGCAAAGTGGTGACTGAGCGGGTTTTCAAACGCCAGCTTGATATACTGCAAGTGGGCATTCGCTGCGACGTTGATAGTGAACCGTGCCCCGGTAAAATGACGAg                                                           <  2:600379/136‑1 (MQ=255)                                          gTGGCATCCTCAGCCAGCAACAAATCGTTATGAGCAAAGTGGTGACTGAGCGGGTTTTCAAACGCCAGCTTGATATACTGCAAGTGGGCATTCGCTGCGACGTTGATAGTGAACCGTGCCCCGGTAAAATGACGAg                                                           >  1:600379/1‑136 (MQ=255)                                                  cTCAGCCAGCAACAAATCGTTATGAGCAAAGTGGTGACTGAGCGGGTTTTCAAACGCCAGCTTGATATACTGCAAGTGGGCATTCGCTGCGACGTTGATAGTGAACCGTGCCCCGGTAAAATGACGAGCATCATTCAgg                                                >  2:524189/1‑139 (MQ=255)                                                                         gAGCAAAGTGGTGACTGAGCGGGTTTTCAAACGCCAGCTTGATATACTGCAAGTGGGCATTCGCTGCGACGTTGATAGTGAACCGTGCCCCGGTAAAATGACGAGCATCATTCAGGCTGACAAAATGTTCGATCACCGt                         <  2:434072/139‑1 (MQ=255)                                                                                         gAGCGGGTTTTCAAACGCCAGCTTGATATACTGCAAGTGGGCATTCGCTGCGACGTTGATAGTGAACCGTGCCCCGGTAAAATGACGAGCATCATTCAGGc                                               >  1:177123/1‑101 (MQ=255)                                                                                         gAGCGGGTTTTCAAACGCCAGCTTGATATACTGCAAGTGGGCATTCGCTGCGACGTTGATAGTGAACCGTGCCCCGGTAAAATGACGAGCATCATTCAGGc                                               <  2:177123/101‑1 (MQ=255)                                                                                          aGCGGGTTTTCAAACGCCAGCTTGATATACTGCAAGTGGGCATTCGCTGCGACGTTGATAGTGAACCGTGCCCCAGTAAAATGACGAGCATCATTCAGGCTGACAAAATGTTCGATCACCGTTGCTTCGGCACCTTCCg        >  1:386287/1‑139 (MQ=255)                                                                                           gCGGGTTTTCAAACGCCAGCTTGATATACTGCAAGTGGGCATTCGCTGCGACGTTGATAGTGAACCGTGCCCCGGTAAAATGACGAGCATCATTCAGGCTGACAAAATGTTCGATCACCGTTGCTTCGGCACCTTCCGc       <  2:386287/139‑1 (MQ=255)                                                                                            cGGGTTTTCAAACGCCAGCTTGATATACTGCAAGTGGGCATTCGCTGCGACGTTGATAGTGAACCGTGCCCCGGTAAAATGACGAGCATCATTCAGGCTGa                                            <  1:636369/101‑1 (MQ=255)                                                                                            cGGGTTTTCAAACGCCAGCTTGATATACTGCAAGTGGGCATTCGCTGCGACGTTGATAGTGAACCGTGCCCCGGTAAAATGACGAGCATCATTCAGGCTGa                                            >  2:636369/1‑101 (MQ=255)                                                                                            cGGGTTTTCAAACGCCAGCTTGATATACTGCAAGTGGGCATTCGCTGCGACGTTGATAGTGAACCGTGCCCCGGTAAAATGACGAGCATCATTCAGGCTGACAAAATGTTCGATCACCGTTCCTTCGGCACCTTCCGcc      <  1:524189/139‑1 (MQ=255)                                                                                                ttttCAAACGCCAGCTTGATATACTGCAAGTGGGCATTCGCTGCGACGTTGATAGTGAACCGTGCCCCGGTAAAATGACGAGCATCATTCAGGCTGACAAAATGTTCGATCACCGTTGCTTCGGCACCTTCCGccagat  >  2:473008/1‑139 (MQ=255)                                                                                                          ccAGCTTGATATACTGCAAGTGGGCATTCGCTGCGACGTTg                                                                                          <  1:573176/41‑1 (MQ=255)                                                                                                          ccAGCTTGATATACTGCAAGTGGGCATTCGCTGCGACGTTg                                                                                          >  2:573176/1‑41 (MQ=255)                                                                                                                      |                                                                                                                     GTAACACTGCGCCACCCAGCAGGAAACTGTGGCTAAATGCGGTGGCATCCTCAGCCAGCAACAAATCGTTATGAGCAAAGTGGTGACTGAGCGGGTTTTCAAACGCCAGCTTGATATGCTGCAAGTGGGCATTCGCTGCGACGTTGATAGTGAACCGTGCCCCGGTAAAATGACGAGCATCATTCAGGCTGACAAAATGTTCGATCACCGTTGCTTCGGCACCTTCCGCCAGAT  >  NC_000913/1761032‑1761265

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑