Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 169,645 A→G Y721C (TAC→TGC)  fhuA → ferrichrome outer membrane transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913169,6450AG100.0% 95.7 / NA 27Y721C (TAC→TGC) fhuAferrichrome outer membrane transporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (14/13);  total (14/13)

GGTGATCCGGCTAACTCCTTTAAAGTGGGAAGTTATACGGTCGTGGATGCGTTAGTACGTTATGATCTGGCGCGAGTCGGCATGGCTGGCTCCAACGTGGCGCTGCATGTTAACAACCTGTTCGATCGTGAATACGTCGCCAGCTGCTTTAACACTTATGGCTGCTTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATG  >  NC_000913/169512‑169780
                                                                                                                                     |                                                                                                                                       
ggTGATCCGGCTAACTCCTTTAAAGTGGGAAGTTATACGGTCGTGGATGCGTTAGTACGTTATGATCTGGCGCGAGTCGGCATGGCTGGCTCCAACGTGGCGCTGCATGTTAACAACCTGTTCGATCGTGAATGCGTCg                                                                                                                                    >  1:710136/1‑139 (MQ=255)
      ccGGCTAACTCCTTTAAAGTGGGAAGTTATACGGTCGTGGATGCGTTAGTACGTTATGATCTGGCGCGAGTCGGCATGGCTGGCTCCAACGTGGCGCTGCATGTTAACAACCTGTTCGATCGTGAATGCGTCGCCAgct                                                                                                                              >  1:357592/1‑139 (MQ=255)
                                                            tATGATCTGGCGCGAGTCGGCATGGCTGGCTCCAACGTGGCGCTGCATGTTAACAACCTGTTCGATCGTGAATGCGTCGCCAGCTGCTTTAACACTTATGgc                                                                                                             >  1:820317/1‑102 (MQ=255)
                                                            tATGATCTGGCGCGAGTCGGCATGGCTGGCTCCAACGTGGCGCTGCATGTTAACAACCTGTTCGATCGTGAATGCGTCGCCAGCTGCTTTAACACTTATGgc                                                                                                             <  2:820317/102‑1 (MQ=255)
                                                                    ggCGCGAGTCGGCATGGCTGGCTCCAACGTGGCGCTGCATGTTAACAACCTGTTCGATCGTGAATGCGTCGCCAGCTGCTTTAACACTTATGgc                                                                                                             <  2:216074/94‑1 (MQ=255)
                                                                    ggCGCGAGTCGGCATGGCTGGCTCCAACGTGGCGCTGCATGTTAACAACCTGTTCGATCGTGAATGCGTCGCCAGCTGCTTTAACACTTATGgc                                                                                                             >  1:216074/1‑94 (MQ=255)
                                                                     gcgcGAGTCGGCATGGCTGGCTCCAACGTGGCGCTGCATGTTAACAACCTGTTCGATCGTGAATGCGTCGCCAGCTGCTTTaa                                                                                                                       <  2:624712/83‑1 (MQ=255)
                                                                     gcgcGAGTCGGCATGGCTGGCTCCAACGTGGCGCTGCATGTTAACAACCTGTTCGATCGTGAATGCGTCGCCAGCTGCTTTaa                                                                                                                       >  1:624712/1‑83 (MQ=255)
                                                                        cgAGTCGGCATGGCTGGCTCCAACGTGGCGCTGCATGTTAACAACCTGTTCGATCGTGAATGCGTCGCCAGCTGCTTTAACACTTATGGCTGCTTCt                                                                                                      >  1:111313/1‑97 (MQ=255)
                                                                        cgAGTCGGCATGGCTGGCTCCAACGTGGCGCTGCATGTTAACAACCTGTTCGATCGTGAATGCGTCGCCAGCTGCTTTAACACTTATGGCTGCTTCt                                                                                                      <  2:111313/97‑1 (MQ=255)
                                                                                     ctggctCCAACGTGGCGCTGCATGTTAACAACCTGTTCGATCGTGAATGCGTCGCCAgc                                                                                                                               <  1:55275/59‑1 (MQ=255)
                                                                                     ctggctCCAACGTGGCGCTGCATGTTAACAACCTGTTCGATCGTGAATGCGTCGCCAgc                                                                                                                               >  2:55275/1‑59 (MQ=255)
                                                                                                   gcgcTGCATGTTAACAACCTGTTCGATCGTGAATGCGTCGCCAGCTGCTTTAACACTTATGGCTGCTTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTggg                                          <  1:248320/130‑1 (MQ=255)
                                                                                                   gcgcTGCATGTTAACAACCTGTTCGATCGTGAATGCGTCGCCAGCTGCTTTAACACTTATGGCTGCTTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTggg                                          >  2:248320/1‑130 (MQ=255)
                                                                                                              tAACAACCTGTTCGATCGTGAATGCGTCGCCAGCTGCTTTAaca                                                                                                                     <  1:463055/44‑1 (MQ=255)
                                                                                                              tAACAACCTGTTCGATCGTGAATGCGTCGCCAGCTGCTTTAaca                                                                                                                     >  2:463055/1‑44 (MQ=255)
                                                                                                                   acCTGTTCGATCGTGAATGCGTCGCCAGCTGCTTTaa                                                                                                                       <  1:483614/37‑1 (MQ=255)
                                                                                                                   acCTGTTCGATCGTGAATGCGTCGCCAGCTGCTTTaa                                                                                                                       >  2:483614/1‑37 (MQ=255)
                                                                                                                   acCTGTTCGATCGTGAATGCGTCGCCAGCTGCTTTAACACTTATGGCTGCTTCTGGGgcg                                                                                                >  2:467160/1‑60 (MQ=255)
                                                                                                                   acCTGTTCGATCGTGAATGCGTCGCCAGCTGCTTTAACACTTATGGCTGCTTCTGGGgcg                                                                                                >  2:477472/1‑60 (MQ=255)
                                                                                                                   acCTGTTCGATCGTGAATGCGTCGCCAGCTGCTTTAACACTTATGGCTGCTTCTGGGgcg                                                                                                <  1:477472/60‑1 (MQ=255)
                                                                                                                   acCTGTTCGATCGTGAATGCGTCGCCAGCTGCTTTAACACTTATGGCTGCTTCTGGGgcg                                                                                                <  1:467160/60‑1 (MQ=255)
                                                                                                                      tGTTCGATCGTGAATGCGTCGCCAGCTGCTTTAACACTTATGGCTGCTTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAg              >  1:572458/1‑139 (MQ=255)
                                                                                                                      tGTTCGATCGTGAATGCGTCGCCAGCTGCTTTAACACTTATGGCTGCTTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAg              <  2:608080/139‑1 (MQ=255)
                                                                                                                             tCGTGAATGCGTCGCCAGCTGCTTTAACACTTATGGCTGCTTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTg       <  2:572458/139‑1 (MQ=255)
                                                                                                                              cGTGAATGCGTCGCCAGCTGCTTTAACACTTATGGCTGCTTCTAGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGt      >  2:736815/1‑139 (MQ=255)
                                                                                                                                  aaTGCGTCGCCAGCTGCTTTAACACTTATGGCTGCTTCTAGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATg  <  1:736815/139‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                       
GGTGATCCGGCTAACTCCTTTAAAGTGGGAAGTTATACGGTCGTGGATGCGTTAGTACGTTATGATCTGGCGCGAGTCGGCATGGCTGGCTCCAACGTGGCGCTGCATGTTAACAACCTGTTCGATCGTGAATACGTCGCCAGCTGCTTTAACACTTATGGCTGCTTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATG  >  NC_000913/169512‑169780

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: