Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,874,078	C→T	*35* (TAG→TAA)	yoaI ←	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,874,078	0	C	T	100.0%	70.8 / NA	23	*35* (TAG→TAA)	yoaI	uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (11/12);  total (11/12)

CCTCTACTCTTCGAACGATTTGATGAAATCGCCTTCAATGCAGGCATGCTGGATAAATCCGTTATTTTGAAAACCGCGGATTATTTGCGCATTGCGCAACCAGAACTGGTCAATTTCCGCCGCACTGCGTAACTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATG  >  NC_000913/1873946‑1874188                                                                                                                                     |                                                                                                               ccTCTGCTCTTCGAACGATTTGATGAAATCGCCTTCAATGCAGGCATGCTGGATAAATCCGTTATTTTGAAAACCGCGGATTATTTGCGCATTGCGCAACCAGAACTGGTCAATTTCCGCCGCACTGCGTAATTAGCCg                                                                                                          <  2:669181/139‑1 (MQ=255)          ttCGAACGATTTGATGAAATCGCCTTCAATGCAGGCATGCTGGATAAATCCGTTATTTTGAAAACCGCGGATTATTTGCGCATTGCGCAACCAGAACTGGTCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCg                                                                                                 >  1:411031/1‑139 (MQ=255)                                                          tCCGTTATTTTGAAAACCGCGGATTATTTGCGCATTGCGCAACCAGAACTGGTCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAACGAt                                                 >  1:762357/1‑139 (MQ=255)                                                                           cgcgGATTATTTGCGCATTGCGCAACCAGAACTGGTCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAgtgt                                >  2:669442/1‑139 (MQ=255)                                                                                        cgcattgcgcaACCAGAACTGGTCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACAtt                   <  1:669442/139‑1 (MQ=255)                                                                                          cattgcgcaACCAGAACTGGTCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGAc                                                                                  <  2:370802/74‑1 (MQ=255)                                                                                          cattgcgcaACCAGAACTGGTCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGAc                                                                                  >  1:370802/1‑74 (MQ=255)                                                                                            ttgcgcaACCAGAACTGGTCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAGCGATGACGTGATAATCAGTGTCTAGACAAACATTTGAt               <  2:40454/139‑1 (MQ=255)                                                                                            ttgcgcaACCAGAACTGGTCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGAt               <  2:411031/139‑1 (MQ=255)                                                                                              gcgcaACCAGAACTGGTCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGAt               >  2:335753/1‑137 (MQ=255)                                                                                              gcgcaACCAGAACTGGTCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGAt               <  1:335753/137‑1 (MQ=255)                                                                                                gcaACCAGAACTGGTCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGtt           <  1:565528/139‑1 (MQ=255)                                                                                                       gAACTGGTCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGAt                                                                                               <  2:264108/48‑1 (MQ=255)                                                                                                       gAACTGGTCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGAt                                                                                               >  1:264108/1‑48 (MQ=255)                                                                                                         aCTGGTCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATg  >  2:535346/1‑139 (MQ=255)                                                                                                            ggTCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGACaaaaa                                                                             <  1:515731/61‑2 (MQ=255)                                                                                                            ggTCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAAc                                                                             >  2:515731/1‑61 (MQ=255)                                                                                                              tCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGATAAgaagaa                                                                                       >  2:208212/1‑49 (MQ=255)                                                                                                              tCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGATAAgaagaa                                                                                       <  1:208212/49‑1 (MQ=255)                                                                                                                       gccgcACTGCGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGAc                                                                                  >  1:731898/1‑45 (MQ=255)                                                                                                                       gccgcACTGCGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGAc                                                                                  <  2:731898/45‑1 (MQ=255)                                                                                                                                cGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCaaa                                                        >  2:400840/1‑62 (MQ=255)                                                                                                                                cGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCaaa                                                        <  1:400840/62‑1 (MQ=255)                                                                                                                                     |                                                                                                               CCTCTACTCTTCGAACGATTTGATGAAATCGCCTTCAATGCAGGCATGCTGGATAAATCCGTTATTTTGAAAACCGCGGATTATTTGCGCATTGCGCAACCAGAACTGGTCAATTTCCGCCGCACTGCGTAACTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATG  >  NC_000913/1873946‑1874188

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑