Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,028,479 G→A A296A (GCC→GCT yedI ← DUF808 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,028,4790GA100.0% 69.2 / NA 23A296A (GCC→GCTyedIDUF808 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (10/13);  total (10/13)

CCATAAAGCTCATACTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTCTCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTTGCTGCCCGGCGAAATGTTCAATGGCGTGAT  >  NC_000913/2028358‑2028600
                                                                                                                         |                                                                                                                         
ccATAAAGCTCATACTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTCTCTACAGCTTTCACTCCCAGcac                                                                                                          <  1:752983/139‑1 (MQ=255)
                  cctcctTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTCTCTACAGCTTTCACTCCCAGCACCACGATGCCGCCgatgat                                                                                        <  2:64684/139‑1 (MQ=255)
                               aGTGAAACTTGTTCACCTTAGTTGAAGATGGCGTAGTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGGCCGCGCAGTTTCTCTACAGCTGTCACTCCCAGCACCACAATGCCGCCGATGATAAATCCAAGAATc                                                                           <  2:509989/139‑1 (MQ=255)
                                                    ttGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTCTCTACAGCTTTCACTCCCAGcac                                                                                                          <  2:650926/87‑1 (MQ=255)
                                                    ttGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTCTCTACAGCTTTCACTCCCAGcac                                                                                                          >  1:650926/1‑87 (MQ=255)
                                                            ggCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTCTCTACAGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGc                                              <  1:155206/139‑1 (MQ=255)
                                                                                   tGCGAGTATTAATGTGCCTGACCGCGCATTTTCTCTACAGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTTGCTGCCCgg                       >  2:281294/1‑139 (MQ=255)
                                                                                     cGAGTATTAATGTGCCTGACCGCGCATTTTCTCTACAGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTTGCTGCCCGGCg                     <  2:283069/139‑1 (MQ=255)
                                                                                     cGAGTATTAATGTGCCTGACCGCGCATTTTCTCTACAGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTTGCTGCCCGGCg                     <  2:348214/139‑1 (MQ=255)
                                                                                            tAATGTGCCTGACCGCGCATTTTCTCTACAGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTTGCTGCCCGGCGAAATGtt              <  1:281294/139‑1 (MQ=255)
                                                                                                 tgCCTGACCGCGCATTTTCTCTACAGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTTGCTGCCCGGCGAAATGTTCAATg         >  1:839055/1‑139 (MQ=255)
                                                                                                   ccTGACCGCGCATTTTCTCTACAGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATatcat                                                 <  1:462286/97‑1 (MQ=255)
                                                                                                   ccTGACCGCGCATTTTCTCTACAGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATatcat                                                 >  2:462286/1‑97 (MQ=255)
                                                                                                   ccTGACCGCGCATTTTCTCTACAGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTTGCTGCCCGGCGAAATGTTCAATGGc       >  1:638661/1‑139 (MQ=255)
                                                                                                     tGACCGCGCATTTTCTCTACAGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTaaa                                                                  <  1:204819/78‑1 (MQ=255)
                                                                                                     tGACCGCGCATTTTCTCTACAGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTaaa                                                                  >  2:204819/1‑78 (MQ=255)
                                                                                                       aCCGCGCATTTTCTCTACAGCTTTCACTCCCAGCACCACGATgccgcc                                                                                              >  2:796785/1‑48 (MQ=255)
                                                                                                       aCCGCGCATTTTCTCTACAGCTTTCACTCCCAGCACCACGATgccgcc                                                                                              <  1:796785/48‑1 (MQ=255)
                                                                                                       aCCGCGCATTTTCTCTACAGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTtgctgc                           <  1:722704/115‑1 (MQ=255)
                                                                                                       aCCGCGCATTTTCTCTACAGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTtgctgc                           >  2:722704/1‑115 (MQ=255)
                                                                                                        ccGCGCATTTTCTCTACAGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTTGCTGCCCGGCGAAATGTTCAATGGCGTGAt  >  2:465780/1‑139 (MQ=255)
                                                                                                           cgcATTTTCTCTACAGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGt                                                             <  2:155029/77‑1 (MQ=255)
                                                                                                           cgcATTTTCTCTACAGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGt                                                             >  1:155029/1‑77 (MQ=255)
                                                                                                                         |                                                                                                                         
CCATAAAGCTCATACTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTCTCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTTGCTGCCCGGCGAAATGTTCAATGGCGTGAT  >  NC_000913/2028358‑2028600

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: