Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	200,214	C→T	P763S (CCA→TCA)	bamA →	BamABCDE complex OM biogenesis outer membrane pore‑forming assembly factor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	200,214	0	C	T	100.0%	130.4 / NA	41	P763S (CCA→TCA)	bamA	BamABCDE complex OM biogenesis outer membrane pore‑forming assembly factor
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (21/20);  total (21/20)

CACCCCGACGCCGTTTATTAGCGATAAGTATGCTAACTCGGTTCGTACTTCCTTCTTCTGGGATATGGGTACCGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATCCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTGGGGCCGTTGGTGTTCTCCTACGCCCAGCCGTTCAAAAAGTACGATGGAGACAAGGCAGAACAGTTCCAGTTTAACATCG  >  NC_000913/200084‑200346                                                                                                                                   |                                                                                                                                     caCCCCGACGCCGTTTATTAGCGATAAGTATGCTAACTCGGTTCGTACTTCCTTCTTCTGGGATATGGGTACCGTTTGGGATACAAACAGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAat                                                                                                                              >  1:531406/1‑139 (MQ=255)                              aTGCTATCTCGGTTCGTACTTCCTTCTTCTGGGATACGGGTACCGTTTGGGTTACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACa                                                                                                 <  2:829876/139‑1 (MQ=255)                               tGCTAACTCGGTTCGTACTTCCTTCTTCTGGGATATGGGTACCGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACaa                                                                                                <  2:447129/139‑1 (MQ=255)                                  tAACTCGGTTCGTACTTCCTTCTTCTGGTATATGGGTACCGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATgg                                                                                             <  2:638681/139‑1 (MQ=255)                                       cGGTTCGTACTTCCTTCTTCTGGGATATGGGTACCGTTTGGGATACAAACTGGGATTCCAGGCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTc                                                                                        <  2:531406/139‑1 (MQ=255)                                         gTTCGTACTTCCTTCTTCTGGGATATGGGTACCGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTccc                                                                                      >  1:207466/1‑139 (MQ=255)                                            cGTACTTCCTTCTTCTGGGATATGGGTACCGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCggg                                                                                                             <  2:552019/113‑1 (MQ=255)                                            cGTACTTCCTTCTTCTGGGATATGGGTACCGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCggg                                                                                                             >  1:552019/1‑113 (MQ=255)                                                   ccttcTTCTGGGATATGGGTACCGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTGGGGcc                                                                            >  1:646436/1‑139 (MQ=255)                                                     ttcttcTGGGATATGGGTACCGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTGGGGCCGt                                                                          <  1:4331/139‑1 (MQ=255)                                                      tcttctGGGATATGGGTACCGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTa                                                                                                   >  1:188066/1‑113 (MQ=255)                                                      tcttctGGGATATGGGTACCGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTa                                                                                                   <  2:188066/113‑1 (MQ=255)                                                         tctGGGATATGGGTACCGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTGGGGCCGTTGgt                                                                      >  2:712806/1‑139 (MQ=255)                                                         tctGGGATATGGGTACCGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTGGGGCCGTTGgt                                                                      >  1:726829/1‑139 (MQ=255)                                                             ggATATGGGTACCGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTGGGGCCGt                                                                          >  2:590276/1‑131 (MQ=255)                                                             ggATATGGGTACCGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTGGGGCCGt                                                                          <  1:590276/131‑1 (MQ=255)                                                                  tGGGTACCGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAat                                                                                                                              <  1:52712/74‑1 (MQ=255)                                                                  tGGGTACCGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAat                                                                                                                              >  2:52712/1‑74 (MQ=255)                                                                    ggTACCGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTGGGGCCGTTGGTGTTCTCCTACg                                                           >  2:187606/1‑139 (MQ=255)                                                                        ccGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAg                                                                                                                                  >  2:511376/1‑64 (MQ=255)                                                                        ccGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAg                                                                                                                                  <  1:511376/64‑1 (MQ=255)                                                                           tttGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAat                                                                                                                              <  2:105938/65‑1 (MQ=255)                                                                           tttGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAat                                                                                                                              >  1:105938/1‑65 (MQ=255)                                                                           tttGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTGGGGCCGTTGGTGTTCTCCTACGCCCAGcc                                                    >  1:782943/1‑139 (MQ=255)                                                                             tGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTGGGGCCGTTGGTGTTCTCCTACGCCCAGCCGt                                                  >  2:409226/1‑139 (MQ=255)                                                                                      aaCTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTGGGGCCGTTGGTGTTCTCCTACGCCCAGCCGTTCAAAAAGt                                         <  1:409226/139‑1 (MQ=255)                                                                                       aCTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTGGGGCCGTTGGTGTTCTCCTACGCCCAGCCGTTCAAAAAGTa                                        <  2:646436/139‑1 (MQ=255)                                                                                                ccAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTGGGGCCGTTGGTGTTCTCCTACGCCCAGCCGTTCAAAAAGTACGATGgaga                               <  2:782943/139‑1 (MQ=255)                                                                                                ccAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTGGGGCCGTTGGTGTTCTCCTACGCCCAGCCGTTCAAAAAGTACGATGgaga                               <  2:774626/139‑1 (MQ=255)                                                                                                 cAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTGGGGCCGt                                                                          <  2:437252/95‑1 (MQ=255)                                                                                                 cAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTGGGGCCGt                                                                          >  1:437252/1‑95 (MQ=255)                                                                                                   gCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTa                                                                                                           >  2:378989/1‑60 (MQ=255)                                                                                                   gCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTa                                                                                                           <  1:378989/60‑1 (MQ=255)                                                                                                             tGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGa                                                                                            <  2:768894/65‑1 (MQ=255)                                                                                                             tGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGa                                                                                            >  1:768894/1‑65 (MQ=255)                                                                                                                  atCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTGGGGCCGTTGGTGTTCTCCTACGCCCAGCCGTTCAAAAAGTACGATGGAGACAAGGCAGAACAGTTc               <  2:682025/137‑1 (MQ=255)                                                                                                                  atCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTGGGGCCGTTGGTGTTCTCCTACGCCCAGCCGTTCAAAAAGTACGATGGAGACAAGGCAGAACAGTTc               >  1:682025/1‑137 (MQ=255)                                                                                                                    ccGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTGGGGCTGTTGGTGtt                                                                   <  2:664908/83‑1 (MQ=255)                                                                                                                    ccGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTGGGGCTGTTGGTGtt                                                                   >  1:664908/1‑83 (MQ=255)                                                                                                                    ccGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTGGGGCCGTTGGTGTTCTCCTACGCCCAGCCGTTCAAAAAGTACGATGGAGACAAGGCAGAACAGTTCCAGt           >  1:473074/1‑139 (MQ=255)                                                                                                                             aGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACACTGGATGTCCCCATTGGGGCCGTTGGTGTTCTCCTACGCCCAGCCGTTCAAAAAGTACGATGGAGACAAGGCAGAACAGTTCCAGTTTAACATCg  >  2:592859/1‑139 (MQ=255)                                                                                                                                   |                                                                                                                                     CACCCCGACGCCGTTTATTAGCGATAAGTATGCTAACTCGGTTCGTACTTCCTTCTTCTGGGATATGGGTACCGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATCCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTGGGGCCGTTGGTGTTCTCCTACGCCCAGCCGTTCAAAAAGTACGATGGAGACAAGGCAGAACAGTTCCAGTTTAACATCG  >  NC_000913/200084‑200346

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑