Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,182,061	G→A	*322* (TAG→TAA)  L241L (CTA→TTA)	yegV → yegW ←	putative kinase putative DNA‑binding transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,182,061	0	G	A	100.0%	117.4 / NA	37	*322* (TAG→TAA)  L241L (CTA→TTA)	yegV yegW	putative kinase putative DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (18/19);  total (18/19)

GCCTCTGGTCTGCCACTGGCGGATGCCGTATTACTGGGCAATGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCACTTGCTTGATCACCAGCACCGGTATTTTGCTATCTAACTGAATATGTGACTGAAACTCGGC  >  NC_000913/2181933‑2182181                                                                                                                                 |                                                                                                                         gCCTCTGGTCTGCCACTGGCGGATGCCGTATTACTGGGCAATGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAATTACTCCTCGCACACAAAAACGTATAAATCGCTGCGa                                                                                                                >  2:329097/1‑139 (MQ=255)              cACTGGCGGATGCCGTATTACTGGGCAATGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAATTACTCCTCGCACACAAAAACGTATAAATCGCTGCGACAGTGGCTAATGc                                                                                                   <  2:782853/139‑1 (MQ=255)              cACTGGCGGATGCCGTATTACTGGGCAATGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAATTACTCCTCGCACACAAAAACGTATAAATCGCTGCGACAGTGGCTAATGc                                                                                                   <  2:335994/139‑1 (MQ=255)                cTGGCGGATGCCGTATTACTGGGCAATGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAATTACTCCTCGCACACAAAAACGTATAAATCGCTGCGACa                                                                                                              >  1:380138/1‑126 (MQ=255)                cTGGCGGATGCCGTATTACTGGGCAATGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAATTACTCCTCGCACACAAAAACGTATAAATCGCTGCGACa                                                                                                              <  2:380138/126‑1 (MQ=255)                           cGTATTACTGGGCAATGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAATTACTCCTCGCACACAAAAACGTATAAATCGCTGCGTCAGTGGc                                                                                                         >  1:65580/1‑120 (MQ=255)                           cGTATTACTGGGCAATGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAATTACTCCTCGCACACAAAAACGTATAAATCGCTGCGACAGTGGc                                                                                                         <  2:65580/120‑1 (MQ=255)                                          tGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAATTACTCCTCGCACACAAAAACGTATAAATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTc                                                                       >  2:227430/1‑139 (MQ=255)                                          tGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAATTACTCCTCGCACACAAAAACGTATAAATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTc                                                                       >  1:365536/1‑139 (MQ=255)                                          tGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAATTACTCCTCGCACACAAAAACGTATAAATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTc                                                                       >  2:48270/1‑139 (MQ=255)                                          tGCAGTGGCGTCGTGGGTAGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAATTACTCCTCGCACACAAAAACGTATAAATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTc                                                                       >  2:466774/1‑139 (MQ=255)                                               tGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAATTACTCCTCGCACACAAAAACGTATAAATCGCTGCGACAGTGGCTa                                                                                                       >  2:214696/1‑102 (MQ=255)                                               tGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAATTACTCCTCGCACACAAAAACGTATAAATCGCTGCGACAGTGGCTa                                                                                                       <  1:214696/102‑1 (MQ=255)                                                  cgtcgtGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAATTACTCCTCGCACACAAAAACGTATAAATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCa                                                               <  2:534748/139‑1 (MQ=255)                                                  cgtcgtGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAATTACTCCTCGCACACAAAAACGTATAAATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCa                                                               <  1:237686/139‑1 (MQ=255)                                                       tGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAATTACTCCTCGCACACAAAAACGTATAAATCGCTGCGACa                                                                                                              >  2:217087/1‑87 (MQ=255)                                                       tGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAATTACTCCTCGCACACAAAAACGTATAAATCGCTGCGACa                                                                                                              <  1:217087/87‑1 (MQ=255)                                                                       ggggCGGTGATTGTGCGCCAACGCGCGAGGAATTACTCCTCGCACACAAAAACGTATAAATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCACTTGCTTGATCACCAGCACCg                                          <  1:48270/139‑1 (MQ=255)                                                                        gggCGGTGATTGTGCGCCAACGCGCGAGGAATTACTCCTCGCACACAAAAACGTATAAATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCACTTGCTTGATCACCAGCACCgg                                         >  1:678817/1‑139 (MQ=255)                                                                               gATTGTGCGCCAACGCGCGAGGAATTACTCCTCGCACACAAAAACGTATAAATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCACTTGCTTGATCACCAGCACCGGTATTTTg                                  <  2:365536/139‑1 (MQ=255)                                                                                  tgtgCGCCAACGCGCGAGGAATTACTCCTCGCACACAAAAACGTATAAATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCACTTGCTTGATCACCAGCACCGGTATTTTGCTa                               <  2:678817/139‑1 (MQ=255)                                                                                   gtgCGCCAACGCGCGAGGAATTACTCCTCGCACACAAAAACGTATAAATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCACTTGCTTGATCACCAGCACCGGTATTTTGCTAt                              <  1:466774/139‑1 (MQ=255)                                                                                    tgCGCCAACGCGCGAGGAATTACTCCTCGCACACAAAAACGTATAAATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAg                                                                    >  2:802210/1‑100 (MQ=255)                                                                                    tgCGCCAACGCGCGAGGAATTACTCCTCGCACACAAAAACGTATAAATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAg                                                                    <  1:802210/100‑1 (MQ=255)                                                                                     gcgcCAACGCGCGAGGAATTACTCCTCGCACACAAAAACGTATAAATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCACTTGCTTGATCACCAGCACCGGTATTTTGCTATCt                            <  1:227430/139‑1 (MQ=255)                                                                                              ctcgAGGAATTACTCCTCGCACACAAAAACGTATAAATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCACTTGCTTGCTCACCAGCACCGGTATTTTg                                  <  1:569544/122‑1 (MQ=255)                                                                                              cgcgAGGAATTACTCCTCGCACACAAAAACGTATAAATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCACTTGCTTGATCACCAGCACCGGTATTTTg                                  >  2:569544/1‑124 (MQ=255)                                                                                                             ctcGCACACAAAAACGTATAAATCGCTGCGACAGTGGCTAATGCt                                                                                                  >  2:217811/1‑45 (MQ=255)                                                                                                             ctcGCACACAAAAACGTATAAATCGCTGCGACAGTGGCTAATGCt                                                                                                  <  1:217811/45‑1 (MQ=255)                                                                                                             ctcGCACACAAAAACGTATAAATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAg                                                                    >  1:785896/1‑75 (MQ=255)                                                                                                             ctcGCACACAAAAACGTATAAATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAg                                                                    <  2:785896/75‑1 (MQ=255)                                                                                                             ctcGCACACAAAAACGTATAAATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCACTTGCTTGATCACCAGCACCGGTATTTTGCTATCTAACTGAATATGTGACTGAAACTcg    >  1:670163/1‑139 (MQ=255)                                                                                                               cGCACACAAAAACGTATAAATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCACTTGCTTGATCACCAGCa                                             >  1:647682/1‑96 (MQ=255)                                                                                                               cGCACACAAAAACGTATAAATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCACTTGCTTGATCACCAGCa                                             <  2:647682/96‑1 (MQ=255)                                                                                                               cGCACACAAAAACGTATAAATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCACTTGCTTGATCACCAGCACCGGTATTTTGCTATCTAACTGAATATGTGACTGAAACTcggc  >  2:203987/1‑139 (MQ=255)                                                                                                                         aaCGTATAAATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGTCGTTGCTGTTGGt                                                                        <  1:48799/59‑1 (MQ=255)                                                                                                                         aaCGTATAAATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGTCGTTGCTGTTGGt                                                                        >  2:48799/1‑59 (MQ=255)                                                                                                                                 |                                                                                                                         GCCTCTGGTCTGCCACTGGCGGATGCCGTATTACTGGGCAATGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCACTTGCTTGATCACCAGCACCGGTATTTTGCTATCTAACTGAATATGTGACTGAAACTCGGC  >  NC_000913/2181933‑2182181

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑