Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 220,239 C→T L230L (CTG→CTA metQ ← DL‑methionine transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913220,2390CT100.0% 91.6 / NA 30L230L (CTG→CTAmetQDL‑methionine transporter subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (15/15);  total (15/15)

CTAAAAATTACCAGCCTTTAACAGCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATCAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAGGTGGTATTGATAACTGCC  >  NC_000913/220106‑220335
                                                                                                                                     |                                                                                                
cTAAAAATTACCAGCCTTTAACAGCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATTAGGtt                                                                                             <  2:431185/139‑1 (MQ=255)
                    aCAGCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATTAGGTTTACGTACGGGGACTCTTTAt                                                                         <  2:570786/139‑1 (MQ=255)
                             ccgTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATTAGGTTTACGTACgggg                                                                                  >  1:375839/1‑121 (MQ=255)
                             ccgTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATTAGGTTTACGTACgggg                                                                                  <  2:375839/121‑1 (MQ=255)
                                          tgtttgCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATTAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCttt                                                   >  2:496870/1‑139 (MQ=255)
                                                                gTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATTAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATctgg                             <  2:417810/139‑1 (MQ=255)
                                                                  cAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATTAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATctggct                           <  1:340848/139‑1 (MQ=255)
                                                                     aCTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATTAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCtggctggc                        >  1:387846/1‑139 (MQ=255)
                                                                     aCTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATTAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCtggctggc                        <  2:426726/139‑1 (MQ=255)
                                                                       tGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATTAGGTTTACGTACggg                                                                                   >  1:296253/1‑78 (MQ=255)
                                                                       tGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATTAGGTTTACGTACggg                                                                                   <  2:296253/78‑1 (MQ=255)
                                                                         aTAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATTAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAg                    >  1:731815/1‑139 (MQ=255)
                                                                         aTAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATTAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAg                    >  1:586684/1‑139 (MQ=255)
                                                                              ccTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATTAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGc                                                <  1:743626/106‑1 (MQ=255)
                                                                              ccTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATTAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGc                                                >  2:743626/1‑106 (MQ=255)
                                                                                tGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATTAGGTTTACGTACggg                                                                                   <  2:36211/69‑1 (MQ=255)
                                                                                tGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATTAGGTTTACGTACggg                                                                                   >  1:36211/1‑69 (MQ=255)
                                                                                 ggACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATTGGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGcc                                               <  2:797655/104‑1 (MQ=255)
                                                                                 ggACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATTGGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGcc                                               >  1:797655/1‑104 (MQ=255)
                                                                                   aCGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATTAGGTTTACGTACGTGGAc                                                                                >  1:177859/1‑69 (MQ=255)
                                                                                   aCGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATTAGGTTTACGTACGGGGAc                                                                                <  2:177859/69‑1 (MQ=255)
                                                                                       aTTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATTAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAGGTGGTATTGATAAc      >  2:46152/1‑139 (MQ=255)
                                                                                                    ctcGGCGTCTTTGTTATCTTCACGCGTCACGATTAGGTTTACGTACGGGGActct                                                                             >  1:577704/1‑55 (MQ=255)
                                                                                                    ctcGGCGTCTTTGTTATCTTCACGCGTCACGATTAGGTTTACGTACGGGGActct                                                                             <  2:577704/55‑1 (MQ=255)
                                                                                                                 ttATCTTCACGCGTCACGATTAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATctgg                             <  2:394358/90‑1 (MQ=255)
                                                                                                                 ttATCTTCACGCGTCACGATTAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATctgg                             >  1:394358/1‑90 (MQ=255)
                                                                                                                           gcgTCACGATTAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATctggct                           <  1:432077/82‑1 (MQ=255)
                                                                                                                           gcgTCACGATTAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATctggct                           >  2:432077/1‑82 (MQ=255)
                                                                                                                                aCGATTAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAGGTGGTATTGATAACTGcc  <  2:420191/102‑1 (MQ=255)
                                                                                                                                aCGATTAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAGGTGGTATTGATAACTGcc  >  1:420191/1‑102 (MQ=255)
                                                                                                                                     |                                                                                                
CTAAAAATTACCAGCCTTTAACAGCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATCAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAGGTGGTATTGATAACTGCC  >  NC_000913/220106‑220335

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: