Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,318,019 G→A R620C (CGT→TGT)  rcsC ← hybrid sensory kinase in two‑component regulatory system with RcsB and YojN

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,318,0190GA100.0% 81.8 / NA 27R620C (CGT→TGT) rcsChybrid sensory kinase in two‑component regulatory system with RcsB and YojN
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (12/15);  total (12/15)

ATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTCAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCAC  >  NC_000913/2317883‑2318154
                                                                                                                                        |                                                                                                                                       
aTCAGTTTTTCGCAACTCGCCAGACCCAGACGGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACAGa                                                                                                                                       <  2:475088/139‑1 (MQ=255)
     tttttCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACAGATagag                                                                                                                                  >  1:764747/1‑139 (MQ=255)
                                 gTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCa                                                                                                      >  1:812467/1‑139 (MQ=255)
                                  tCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATg                                                                                                        <  1:414944/136‑1 (MQ=255)
                                  tCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATg                                                                                                        >  2:414944/1‑136 (MQ=255)
                                  tCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCaa                                                                                                     <  2:764747/139‑1 (MQ=255)
                                             ttACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGc                                                                                          <  1:751343/139‑1 (MQ=255)
                                                             ttCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAAttt                                                                          >  1:284463/1‑139 (MQ=255)
                                                               ccGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACtata                                                                                              >  2:50295/1‑117 (MQ=255)
                                                               ccGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACtata                                                                                              <  1:50295/117‑1 (MQ=255)
                                                                                  cAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAAttt                                                                          >  2:700399/1‑118 (MQ=255)
                                                                                  cAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAAttt                                                                          <  1:700399/118‑1 (MQ=255)
                                                                                           tcaccacTTCTTTCGCTGGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGTGAACATGCAAAACTATACAGCTGGTATCGGTGAATTTTATGGCGTTACTCAACAGGTTGGAGATGAc                                            <  2:409177/138‑1 (MQ=255)
                                                                                           gcACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTCAACAGGTTGGAGATGAc                                            <  2:284463/139‑1 (MQ=255)
                                                                                                         gCCGGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTCAACAGGTTGGAGATGACCTGCTGTAAACGCa                              <  1:752246/139‑1 (MQ=255)
                                                                                                                     aCGCCGGTATCGCGAACACAGATAGAGAGATAATCGcc                                                                                                                       <  2:120501/38‑1 (MQ=255)
                                                                                                                     aCGCCGGTATCGCGAACACAGATAGAGAGATAATCGcc                                                                                                                       >  1:120501/1‑38 (MQ=255)
                                                                                                                       gCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATg                                                                                                        <  1:144727/51‑1 (MQ=255)
                                                                                                                       gCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATg                                                                                                        >  2:144727/1‑51 (MQ=255)
                                                                                                                       gCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATg                                                                                                        <  1:192575/51‑1 (MQ=255)
                                                                                                                       gCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATgg                                                                     >  2:14532/1‑86 (MQ=255)
                                                                                                                       gCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATgg                                                                     <  1:14532/86‑1 (MQ=255)
                                                                                                                       gCCGGTATCGCGAACACAGATAGAGAGATAATAGCCATCCGCGCGAACATg                                                                                                        >  2:192575/1‑51 (MQ=255)
                                                                                                                        ccGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGt                                                                  >  2:325105/1‑88 (MQ=255)
                                                                                                                        ccGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGt                                                                  <  1:325105/88‑1 (MQ=255)
                                                                                                                            tATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTCaa                                                           <  1:823887/91‑1 (MQ=255)
                                                                                                                            tATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTCaa                                                           >  2:823887/1‑91 (MQ=255)
                                                                                                                                     cacaGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTCAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGcac  >  2:595455/1‑139 (MQ=255)
                                                                                                                                        |                                                                                                                                       
ATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTCAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCAC  >  NC_000913/2317883‑2318154

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: