Predicted mutation | ||||||
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evidence | seq id | position | mutation | annotation | gene | description |
RA | NC_000913 | 2,318,019 | G→A | R620C (CGT→TGT) | rcsC ← | hybrid sensory kinase in two‑component regulatory system with RcsB and YojN |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 2,318,019 | 0 | G | A | 100.0% | 81.8 / NA | 27 | R620C (CGT→TGT) | rcsC | hybrid sensory kinase in two‑component regulatory system with RcsB and YojN |
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (12/15); total (12/15) |
ATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTCAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCAC > NC_000913/2317883‑2318154 | aTCAGTTTTTCGCAACTCGCCAGACCCAGACGGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACAGa < 2:475088/139‑1 (MQ=255) tttttCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACAGATagag > 1:764747/1‑139 (MQ=255) gTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCa > 1:812467/1‑139 (MQ=255) tCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATg < 1:414944/136‑1 (MQ=255) tCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATg > 2:414944/1‑136 (MQ=255) tCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCaa < 2:764747/139‑1 (MQ=255) ttACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGc < 1:751343/139‑1 (MQ=255) ttCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAAttt > 1:284463/1‑139 (MQ=255) ccGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACtata > 2:50295/1‑117 (MQ=255) ccGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACtata < 1:50295/117‑1 (MQ=255) cAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAAttt > 2:700399/1‑118 (MQ=255) cAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAAttt < 1:700399/118‑1 (MQ=255) tcaccacTTCTTTCGCTGGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGTGAACATGCAAAACTATACAGCTGGTATCGGTGAATTTTATGGCGTTACTCAACAGGTTGGAGATGAc < 2:409177/138‑1 (MQ=255) gcACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTCAACAGGTTGGAGATGAc < 2:284463/139‑1 (MQ=255) gCCGGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTCAACAGGTTGGAGATGACCTGCTGTAAACGCa < 1:752246/139‑1 (MQ=255) aCGCCGGTATCGCGAACACAGATAGAGAGATAATCGcc < 2:120501/38‑1 (MQ=255) aCGCCGGTATCGCGAACACAGATAGAGAGATAATCGcc > 1:120501/1‑38 (MQ=255) gCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATg < 1:144727/51‑1 (MQ=255) gCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATg > 2:144727/1‑51 (MQ=255) gCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATg < 1:192575/51‑1 (MQ=255) gCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATgg > 2:14532/1‑86 (MQ=255) gCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATgg < 1:14532/86‑1 (MQ=255) gCCGGTATCGCGAACACAGATAGAGAGATAATAGCCATCCGCGCGAACATg > 2:192575/1‑51 (MQ=255) ccGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGt > 2:325105/1‑88 (MQ=255) ccGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGt < 1:325105/88‑1 (MQ=255) tATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTCaa < 1:823887/91‑1 (MQ=255) tATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTCaa > 2:823887/1‑91 (MQ=255) cacaGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTCAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGcac > 2:595455/1‑139 (MQ=255) | ATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTCAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCAC > NC_000913/2317883‑2318154 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |