Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,343,422	C→T	A250T (GCT→ACT)	yfaL ←	adhesin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,343,422	0	C	T	100.0%	175.4 / NA	58	A250T (GCT→ACT)	yfaL	adhesin
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (29/29);  total (29/29)

AGATACATAAAGCCACCCGCCGCAGAGGAAGGACCATCTCCATAGCCTGCTGCGCTATTGTTCTCATCGACTAACACGCCTCCGTTCTGGCTGTAGCTGTCATCAACAGAAATATCAATAAGATAGGGAGCCGTCACGCTATTGGTATATATCGCCCCGCCATAACCTTCGGCAGTGTTATTTGTAAAGGCAGTGTTATTTACTATCGTATAACCTGAAGGATGCTTGCTGTCGCTATTATTATCGGTAACATCGATTGCCCCGC  >  NC_000913/2343292‑2343556                                                                                                                                   |                                                                                                                                       agaTACATAAAGCCACCCGCCGCAGAGGAAGGACCATCTCCATAGCCTGCTGCGCTATTGTTCTCATCGACTAACACGCCTCCGTTCTGGCTGTAGCTGTCATCAACAGAAATATCAATAAGATAGGGAGTCGTCACGc                                                                                                                                <  1:162779/139‑1 (MQ=255) agaTACATAAAGCCACCCGCCGCAGAGGAAGGACCATCTCCATAGCCTGCTGCGCTATTGTTCTCATCGACTAACACGCCTCCGTTCTGGCTGTAGCTGTCATCAACAGAAATATCAATAAGATAGGGAGTCGTCACGc                                                                                                                                <  1:710688/139‑1 (MQ=255)   aTACATAAAGCCACCCGCCGCAGAGGAAGGACCATCTCCATAGCCTGCTGCGCTATTGTTCTCATCGACTAACACGCCTCCGTTCTGGCTGTAGCTGTCATCAACAGAAATATCAATAAGATAGGGAGTCGTCa                                                                                                                                   >  2:191965/1‑134 (MQ=255)   aTACATAAAGCCACCCGCCGCAGAGGAAGGACCATCTCCATAGCCTGCTGCGCTATTGTTCTCATCGACTAACACGCCTCCGTTCTGGCTGTAGCTGTCATCAACAGAAATATCAATAAGATAGGGAGTCGTCa                                                                                                                                   <  1:191965/134‑1 (MQ=255)   aTACATAAAGCCACCCGCCGCAGAGGAAGGACCATCTCCATAGCCTGCTGCGCTATTGTTCTCATCGACTAACACGCCTCCGTTCTGGCTGTAGCTGTCATCAACAGAAATATCAATAAGATAGGGAGTCGTCACGCTa                                                                                                                              <  1:633131/139‑1 (MQ=255)       aTAAAGCCACCCGCCGCAGAGGAAGGACCATCTCCATAGCCTGCTGCGCTATTGTTCTCATCGACTAACACGCCTCCGTTCTGGCTGTAGCTGTCATCAACAGAAATATCAATAAGATAGGGAGTCGTCAc                                                                                                                                  <  1:605476/131‑1 (MQ=255)       aTAAAGCCACCCGCCGCAGAGGAAGGACCATCTCCATAGCCTGCTGCGCTATTGTTCTCATCGACTAACACGCCTCCGTTCTGGCTGTAGCTGTCATCAACAGAAATATCAATAAGATAGGGAGTCGTCAc                                                                                                                                  >  2:605476/1‑131 (MQ=255)               aCCCGCCGCAGAGGAAGGACCATCTCCATAGCCTGCTGCGCTATTGTTCTCATCGACTAACACGCCTCCGTTCTGGCTGTAGCTGTCATCAACAGAAATATCAATAAGATAGGGAGTCGTCAc                                                                                                                                  >  2:30099/1‑123 (MQ=255)               aCCCGCCGCAGAGGAAGGACCATCTCCATAGCCTGCTGCGCTATTGTTCTCATCGACTAACACGCCTCCGTTCTGGCTGTAGCTGTCATCAACAGAAATATCAATAAGATAGGGAGTCGTCAc                                                                                                                                  <  1:30099/123‑1 (MQ=255)                cccGCCGCAGAGGAAGGACCATCTCCATAGCCTGCTGCGCTATTGTTCTCATCGACTAACACGCCTCCGTTCTGGCTGTAGCTGTCATCAACAGAAATATCAATAAGATCGGGAgtcgtc                                                                                                                                    >  2:540976/1‑120 (MQ=255)                cccGCCGCAGAGGAAGGACCATCTCCATAGCCTGCTGCGCTATTGTTCTCATCGACTAACACGCCTCCGTTCTGGCTGTAGCTGTCATCAACAGAAATATCAATAAGATAGGGAgtcgtc                                                                                                                                    <  1:540976/120‑1 (MQ=255)                        agagAAAGGACAATCTCCATAGCCTGCTGCGCTATTGTTCTCATCGACTAACACGCCTCCGTTCTGGCTGTAGCTGTCATCAACAGAAATATCAATAAGATAGGGAGTCGTCACGCTATTGGTATATATCGCCCCGCCa                                                                                                         >  2:486766/1‑139 (MQ=255)                                   cATCTCCATAGCCTGCTGCGCTATTGTTCTCATCGACTAACACGCCTCCGTTCTGGCTGTAGCTGTCATCAACAGAAATATCAATAAGATAGGGAGTCGTCACGCTATTGGTATATATCGCCCCGCCATAACCTTCGGc                                                                                              <  2:448248/139‑1 (MQ=255)                                             gCCTGCTGCGCTATTGTTCTCATCGATTAACACGCCTCCGTTCTGGCTGTAGCTGTCATCAACAGAAATATCAATAAGATAGGGAGTCGTCACGCTATTGGTATATATCGc                                                                                                                <  2:149913/111‑1 (MQ=255)                                             gCCTGCTGCGCTATTGTTCTCATCGACTAACACGCCTCCGTTCTGGCTGTAGCTGTCATCAACAGAAATATCAATAAGATAGGGAGTCGTCACGCTATTGGTATATATCGc                                                                                                                >  1:149913/1‑111 (MQ=255)                                                tgctgcGCTATTGTTCTCATCGACTAACACGCCTCCGTTCTGGTTGTAGCTGTCATCAACAGAAATATCAATAAGATAGGGAGTCGTCACGCTATTGGTATATATCGCCCCGCCATAACCtt                                                                                                  <  1:511570/122‑1 (MQ=255)                                                tgctgcGCTATTGTTCTCATCGACTAACACGCCTCCGTTCTGGCTGTAGCTGTCATCAACAGAAATATCAATAAGATAGGGAGTCGTCACGc                                                                                                                                >  1:776037/1‑92 (MQ=255)                                                tgctgcGCTATTGTTCTCATCGACTAACACGCCTCCGTTCTGGCTGTAGCTGTCATCAACAGAAATATCAATAAGATAGGGAGTCGTCACGc                                                                                                                                <  2:776037/92‑1 (MQ=255)                                                tgctgcGCTATTGTTCTCATCGACTAACACGCCTCCGTTCTGGCTGTAGCTGTCATCAACAGAAATATCAATAAGATAGGGAGTCGTCACGCTATTGGTATATATCGCCCCGCCATAACCtt                                                                                                  >  2:511570/1‑122 (MQ=255)                                                tgctgcGCTATTGTTCTCATCGACTAACACGCCTCCGTTCTGGCTGTAGCTGTCATCAACAGAAATATCAATAAGATAGGGAGTCGTCACGCTATTGGTATATATCGCCCCGCCATAACCTTCGGCAGTGTTATTTGTa                                                                                 >  1:319974/1‑139 (MQ=255)                                                 gctgcGCTATTGTTCTCATCGACTAACACGCCTCCGTTCTGGCTGTAGCTGTCATCAACAGAAATATCAATAAGATAGGGAGTCGTCACGCTa                                                                                                                              >  1:358760/1‑93 (MQ=255)                                                 gctgcGCTATTGTTCTCATCGACTAACACGCCTCCGTTCTGGCTGTAGCTGTCATCAACAGAAATATCAATAAGATAGGGAGTCGTCACGCTa                                                                                                                              <  2:358760/93‑1 (MQ=255)                                                   tgcGCTATTGTTCTCATCGACTAACACGCCTCCGTTCTGGCTGTAGCTGTCATCAACAGAAATATCAATAATATAGGGAGTCGTCACGc                                                                                                                                <  2:479666/89‑1 (MQ=255)                                                   tgcGCTATTGTTCTCATCGACTAACACGCCTCCGTTCTGGCTGTAGCTGTCATCAACAGAAATATCAATAAGATAGGGAGTCGTCACGc                                                                                                                                >  1:479666/1‑89 (MQ=255)                                                   tgcGCTATTGTTCTCATCGACTAACACGCCTCCGTTCTGGCTGTAGCTGTCATCAACAGAAATATCAATAAGATAGGGAGTCGTCACGc                                                                                                                                >  1:670139/1‑89 (MQ=255)                                                   tgcGCTATTGTTCTCATCGACTAACACGCCTCCGTTCTGGCTGTAGCTGTCATCAACAGAAATATCAATAAGATAGGGAGTCGTCACGc                                                                                                                                <  2:670139/89‑1 (MQ=255)                                                   tgcGCTATTGTTCTCATCGACTAACACGCCTCCGTTCTGGCTGTAGCTGTCATCAACAGAAATATCAATAAGATAGGGAGTCGTCACGCTATTGGTATATATCGCCCCGCCATAACCTTCGGCAGTGTTATTTGTAAAg                                                                              >  1:450192/1‑139 (MQ=255)                                                               ctcATCGACTAACACGCCTCCGTTCTGGCTGTAGCTGTCATCAACAGAAATATCAATAAGATAGGGAGTCGTCACGCt                                                                                                                               <  1:231780/78‑1 (MQ=255)                                                               ctcATCGACTAACACGCCTCCGTTCTGGCTGTAGCTGTCATCAACAGAAATATCAATAAGATAGGGAGTCGTCACGCt                                                                                                                               >  2:231780/1‑78 (MQ=255)                                                               ctcATCGACTAACACGCCTCCGTTCTGGCTGTAGCTGTCATCAACAGAAATATCAATAAGATAGGGAGTCGTCACGCTa                                                                                                                              <  1:735268/79‑1 (MQ=255)                                                               ctcATCGACTAACACGCCTCCGTTCTGGCTGTAGCTGTCATCAACAGAAATATCAATAAGATAGGGAGTCGTCACGCTa                                                                                                                              >  2:735268/1‑79 (MQ=255)                                                               ctcATCGACTAACACGCCTCCGTTCTGGCTGTAGCTGTCATCAACAGAAATATCAATAAGATAGGGAGTCGTCACGCTATTGGTATATATCGCCCCGCCATAACCTTCGGCAGTGTTATTTGTAAAGGCAg                                                                          <  1:692666/131‑1 (MQ=255)                                                               ctcATCGACTAACACGCCTCCGTTCTGGCTGTAGCTGTCATCAACAGAAATATCAATAAGATAGGGAGTCGTCACGCTATTGGTATATATCGCCCCGCCATAACCTTCGGCAGTGTTATTTGTAAAGGCAg                                                                          >  2:692666/1‑131 (MQ=255)                                                                  aTCGACTAACACGCCTCCGTTCTGGCTGTAGCTGTCATCAACAGAAATATCAATAAGATAGGGAGTCGTCACGCTATTGGTATATATCGCCCCGCCATAACCTTCGGCAgtg                                                                                          >  1:565133/1‑112 (MQ=255)                                                                  aTCGACTAACACGCCTCCGTTCTGGCTGTAGCTGTCATCAACAGAAATATCAATAAGATAGGGAGTCGTCACGCTATTGGTATATATCGCCCCGCCATAACCTTCGGCAgtg                                                                                          <  2:565133/112‑1 (MQ=255)                                                                  aTCGACTAACACGCCTCCGTTCTGGCTGTAGCTGTCATCAACAGAAATATCAATAAGATAGGGAGTCGTCACGCTATTGGTATATATCGCCCCGCCATAACCTTCGGCAGTGTTATTTGTAAAGGCAGTGTTATTTACt                                                               >  1:111598/1‑139 (MQ=255)                                                                       cTAACACGCCTCCGTTCTGGCTGTAGCTGTCATCAACAGAAATATCAATAAGATAGGGAGTCGTCACGCTATTGGTATATATCGCCCCGCCATAACCTTCGGCAGTGTTATTTGTAAAGGCa                                                                           >  1:3202/1‑122 (MQ=255)                                                                       cTAACACGCCTCCGTTCTGGCTGTAGCTGTCATCAACAGAAATATCAATAAGATAGGGAGTCGTCACGCTATTGGTATATATCGCCCCGCCATAACCTTCGGCAGTGTTATTTGTAAAGGCa                                                                           <  2:3202/122‑1 (MQ=255)                                                                         aaCACGCCTCCGTTCTGGCTGTAGCTGTCATCAACAGAAATATCAATAAGATAGGGAGTCGTCACGCTATTGGTATATATCGCCCCGCCATAACCTTCGGCAGTGTTATTTGTAAAGGCAGTGTTATTTACTATCGtat                                                        <  2:319974/139‑1 (MQ=255)                                                                                cTCCGTTCTGGCTGTAGCTGTCATCAACAGAAATATCAATAAGATAGGGAGTCGt                                                                                                                                     <  1:175870/55‑1 (MQ=255)                                                                                cTCCGTTCTGGCTGTAGCTGTCATCAACAGAAATATCAATAAGATAGGGAGTCGt                                                                                                                                     >  2:175870/1‑55 (MQ=255)                                                                                cTCCGTTCTGGCTGTAGCTGTCATCAACAGAAATATCAATAAGATAGGGAGTCGTCACGCTATTGGTATATATCGCCCCGCCATAACCTTCGGCAGTGtt                                                                                        <  1:319169/100‑1 (MQ=255)                                                                                cTCCGTTCTGGCTGTAGCTGTCATCAACAGAAATATCAATAAGATAGGGAGTCGTCACGCTATTGGTATATATCGCCCCGCCATAACCTTCGGCAGTGtt                                                                                        >  2:319169/1‑100 (MQ=255)                                                                                cTCCGTTCTGGCTGTAGCTGTCATCAACAGAAATATCAATAAGATAGGGAGTCGTCACGCTATTGGTATATATCGCCCCGCCATAACCTTCGGCAGTGTTATTTGTAAAGGCAGTGTTATTTACTATCGTATAACCTGa                                                 >  1:749275/1‑139 (MQ=255)                                                                                           cTGTAGCTTTCATAAAAAGAAATATCAATAAGATAGGGAGTCGTCACGCTATTGGTATATATCGCCCCGCCATAACCTTCGGCAGTGTTATTTGTAAAGGCAGTGTTATTTACTATCGTATAACCTGAAGGAtgcttgc                                      <  2:111598/139‑1 (MQ=255)                                                                                               aGCTGTCATCAACAGAAATATCAATAAGATAGGGAGTCGTCACGCTATTGGTATATATCGCCCCGCCATAACCTTCGGCAGTGTTATTTGTAAAGGCAGTGTTATTTACTATCGTATAACCTGAAGGATGCTTGCTGTc                                  <  1:486766/139‑1 (MQ=255)                                                                                                  tGTCATCAACAGAAATATCAATAAGATAGGGAGTCGTCACGCTa                                                                                                                              >  2:126240/1‑44 (MQ=255)                                                                                                  tGTCATCAACAGAAATATCAATAAGATAGGGAGTCGTCACGCTa                                                                                                                              <  1:126240/44‑1 (MQ=255)                                                                                                    tcatcaACAGAAATATCAATAAGATAGGGAGTCGTCACGCTATTGGTATATATCGCCCCGCCATAACCTTCGGCAGTGTTATTTGTAAAGGCAGTGTTAtt                                                                   >  2:372434/1‑101 (MQ=255)                                                                                                    tcaacaACAGAAATATCAATAAGATAGGGAGTCGTCACGCTATTGGTATATATCGCCCCGCCATAACCTTCGGCAGTGTTATTTGTAAAGGCAATGTTATTTACTATCGTATAACCTGAAGGATGCTTGCTGTCGCtat                             >  2:540037/1‑139 (MQ=255)                                                                                                    taatcaACAGAAATATCAATAAGATAGGGAGTCGTCACGCTATTGGTATATATCGCCCCGCCATAACCTTCGGCAGTGTTATTTGTAAAGGCAGTGTTAtt                                                                   <  1:372434/99‑1 (MQ=255)                                                                                                                 tatCAATAAGATAGGGAGTCGTCACGCTATTGGTATATATCGCCCCGCCa                                                                                                         <  2:210190/50‑1 (MQ=255)                                                                                                                 tatCAATAAGATAGGGAGTCGTCACGCTATTGGTATATATCGCCCCGCCa                                                                                                         >  1:210190/1‑50 (MQ=255)                                                                                                                     aaTAAGATAGGGAGTCGTCACGCTATTGGTATATATCGCCCCGCCATAACCTTCGGCa                                                                                             >  1:30185/1‑58 (MQ=255)                                                                                                                     aaTAAGATAGGGAGTCGTAATGCTATTGGTATATATCGCCCCGCCATAACCTTCGGCa                                                                                             <  2:30185/58‑1 (MQ=255)                                                                                                                             aGGGAGTCGTCACGCTATTGGTATATATCGCCCCGCCATAACCTTCGGCAGTGTTATTTGTAAAGGCa                                                                           >  2:124373/1‑68 (MQ=255)                                                                                                                             aGGGAGTCGTCACGCTATTGGTATATATCGCCCCGCCATAACCTTCGGCAGTGTTATTTGTAAAGGCa                                                                           <  1:124373/68‑1 (MQ=255)                                                                                                                               ggAGTCGTCACGCTATTGGTATATATCGCCCCGCCATAACCTTCGGCAGTGTTATTTGTAAAGGCAgtg                                                                        >  2:198342/1‑69 (MQ=255)                                                                                                                               ggAGTCGTCACGCTATTGGTATATATCGCCCCGCCATAACCTTCGGCAGTGTTATTTGTAAAGGCAgtg                                                                        <  1:198342/69‑1 (MQ=255)                                                                                                                               ggAGTCGTCACGCTATTGGTATATATCGCCCCGCCATAACCTTCGGCAGTGTTATTTGTAAAGGCAGTGTTATTTACTATCGTATAACCTGAAGGATGCTTGCTGTCGCTATTATTATCGGTAACATCGATTGCCCcgc  >  1:437306/1‑139 (MQ=255)                                                                                                                                   |                                                                                                                                       AGATACATAAAGCCACCCGCCGCAGAGGAAGGACCATCTCCATAGCCTGCTGCGCTATTGTTCTCATCGACTAACACGCCTCCGTTCTGGCTGTAGCTGTCATCAACAGAAATATCAATAAGATAGGGAGCCGTCACGCTATTGGTATATATCGCCCCGCCATAACCTTCGGCAGTGTTATTTGTAAAGGCAGTGTTATTTACTATCGTATAACCTGAAGGATGCTTGCTGTCGCTATTATTATCGGTAACATCGATTGCCCCGC  >  NC_000913/2343292‑2343556

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑