Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	28,269	C→T	intergenic (+62/‑105)	rihC → / → dapB	ribonucleoside hydrolase 3/dihydrodipicolinate reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	28,269	0	C	T	100.0%	108.0 / NA	35	intergenic (+62/‑105)	rihC/dapB	ribonucleoside hydrolase 3/dihydrodipicolinate reductase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (15/20);  total (15/20)

ATTGGATCTGGATGTGAAAGGCTTCCAGCAGTGGGTGGCTGAGGTGCTGGCTCTGGCGTCGTAACCTGTCACATGTTATTGGCATGCAGTCATTCATCGACTCATGCCTTTCACTGATATCCCTCCCTGTTTATCATTAATTTCTAATTATCAGCGTTTTTGGCTGGCGGCGTAGCGATGCGCTGGTTACTCTGAAAACGGTCTATGCAAATTAACAAAAGAGAATAGCTATGCATGATGCAAACATCCGCGTTGCCA  >  NC_000913/28144‑28401                                                                                                                              |                                                                                                                                     aTTGGATCTGGATGTGAAAGGCTTCCAGCAGTGGGTGGCTGAGGTGCTGGCTCTGGCGTCGTAACCTGTCACATGTTATTGGCATGCAGTCATTCATCGACTCATGCCTTTCACTGATATCCCTCTCTGTTTATCATTa                                                                                                                         >  1:759796/1‑139 (MQ=255)                     gCTTCCAGCAGTGGGTGGCTGAGGTGCTGGCTCTGGCGTCGTAACCTGTCACATGTTATTGGCATGCAGTCATTCATCGACTCATGCCTTTCACTGATATCCCTCTCTGTTTATCATTAATTTCTAATTATCAGCGttt                                                                                                     >  2:674073/1‑139 (MQ=255)                     gCTTCCAGCAGTGGGTGGCTGAGGTGCTGGCTCTGGCGTCGTAACCTGTCACATGTTATTGGCATGCAGTCATTCATCGACTCATGCCTTTCACTGATATCCCTCTCTGTTTATCATTAATTTCTAATTATCAGCGttt                                                                                                     >  1:380381/1‑139 (MQ=255)                     gCTTCCAGCAGTGGGTGGCTGAGGTGCTGGCTCTGGCGTCGTAACCTGTCACATGTTATTGGCATGCAGTCATTCATCGACTCATGCCTTTCACTGATATCCCTCTCTGTTTATCATTAATTTCTAATTATCAGCGttt                                                                                                     >  1:218379/1‑139 (MQ=255)                         ccAGCAGTGGGTGGCTGAGGTGCTGGCTCTGGCGTCGTAACCTGTCACATGTTATTGGCATGCAGTCATTCATCGACTCATGCCTTTCACTGATATCCCTCTCTGTTTATCATTAATTTCTAATTATCAGCGTTTTtgg                                                                                                 >  2:145567/1‑139 (MQ=255)                             cagTGGGTGGCTGAGGTGCTGGCTCTGGCGTCGTAACCTGTCACATGTTATTGGCATGCAGTCATTCATCGACTCATGCCTTTCACTGATATCCCTCTCTGTTTATCATTAATTTCTAATTATCAGCGTTTTtggctgg                                                                                             <  2:249725/139‑1 (MQ=255)                             cagTGGGTGGCTGAGGTGCTGGCTCTGGCGTCGTAACCTGTCACATGTTATTGGCATGCAGTCATTCATCGACTCATGCCTTTCACTGATATCCCTCTCTGTTTATCATTAATTTCTAATTATCAGCGTTTTtggctgg                                                                                             <  2:759796/139‑1 (MQ=255)                                            gTGCTGGCTCTGGCGTCGTAACCTGTCACATGTTATTGGCATGCAGTCATTCATCGACTCATGCCTTTCACTGATATCCCTCTCTGTTTATCATTAATTTCTAATTATCAGCGTTTTTGGCTGGCGGCGTAGCGATgcg                                                                              <  2:496971/139‑1 (MQ=255)                                                    tctGGCGTCGTAACCTGTCACATGTTATTGGCATGCAGTCATTCATCGACTCATGCCTTTCACTGATATCCCTCTCTGTTTATCATTAATTTCTAATTATCAGCGTTTTTGGCTGGCGGCGTAGCGATGCGCTGGTTAc                                                                      >  1:620175/1‑139 (MQ=255)                                                           tcgtAACCTGTCACATGTTATTGGCATGCAGTCATTCATCGACTCATGCCTTTCACTGATATCCCTCTCTGTTTATCATTAATTTCTAATTATCAGCGTTTTTGGCTGGCGGCGTAGCGATGCGCTGGTTACTCTGaaa                                                               <  1:247478/139‑1 (MQ=255)                                                                aCCTGTCACATGTTATTGGCATGCAGTCATTCATCGACTCATGCCTTTCACTGATATCCCTCTCTGTTTATCATT‑ATTTCTAATTATCAGCGTTTTTGGCTGGCGGCGTAGCGATGCGCTGGTTACTCTGAAAACGGTc                                                         >  1:19413/1‑139 (MQ=255)                                                                   tGTCACATGTTATTGGCATGCAGTCATTCATCGACTCATGCCTTTCACTGATATCCCTCTCTGTTTATCATTAATTTCTAATTATCAGCGTTTTTGGCTGGCGGCGTAGCGATGCGCTGGTTACTCTGAAAACGGTCTa                                                       <  1:674073/139‑1 (MQ=255)                                                                               ttGGCATGCAGTCATTCATCGACTCATGCCTTTCACTGATATCCCTCTCTGTTTATCATT‑ATTTCTAATTATCAGCGTTTTTGGCTGGCGGCGTAGCGATGCGCTGGTTACTCTGAAAACGGTCTATGCAAATTAACaa                                          <  2:19413/139‑1 (MQ=255)                                                                                tGGCATGCAGTCATTCATCGACTCATGCCTTTCACTGATATCCCTCTCTGTTTATCATTAATTTCTAATTATCAGCGTTTTTGGCTGGCGGCGTAGCGATGCGCTGGTTACTCTGAAAACGGTCTATGCAAATTAACaa                                          <  2:380381/139‑1 (MQ=255)                                                                                 ggCATGCAGTCATTCATCGACTCATGCCTTTCACTGATATCCCTCTCTGTTTATCATTAATTTCTAATTATCAGCGTTTTTGGCTGGCGGCGTAGCGATGCGCTGGTTACTCTGAAAACGGTCTATGCaa                                                  >  1:201295/1‑130 (MQ=255)                                                                                 ggCATGCAGTCATTCATCGACTCATGCCTTTCACTGATATCCCTCTCTGTTTATCATTAATTTCTAATTATCAGCGTTTTTGGCTGGCGGCGTAGCGATGCGCTGGTTACTCTGAAAACGGTCTATGCaa                                                  <  2:201295/130‑1 (MQ=255)                                                                                  gCATGCAGTCATTCATCGACTCATGCCTTTCACTGATATCCCTCTCTGTTTATCATTAATTTCTAATTATCAGCGTTTTTGGCTGGCGGCGTAGCGATGCGCTGGTTACTCTGAAAACGGTCTATGCaa                                                  <  2:5252/129‑1 (MQ=255)                                                                                  gCATGCAGTCATTCATCGACTCATGCCTTTCACTGATATCCCTCTCTGTTTATCATTAATTTCTAATTATCAGCGTTTTTGGCTGGCGGCGTAGCGATGCGCTGGTTACTCTGAAAACGGTCTATGCaa                                                  >  1:5252/1‑129 (MQ=255)                                                                                  gCATGCAGTCATTCATCGACTCATGCCTTTCACTGATATCCCTCTCTGTTTATCATTAATTTCTAATTATCAGCGTTTTTGGCTGGCGGCGTAGCGATGCGCTGGTTACTCTGAAAACGGTCTATGCAAATTAACaaaa                                        <  2:218379/139‑1 (MQ=255)                                                                                     tGCAGTCATTCATCGACTCATGCCTTTCACTGATATCCCTCTCTGTTTATCATTAATTTCTAATTATCAGCGTTTTTGGCTGGCGGCGTAGCGATGCGCTGGTTACTCTGAAAACGGTCTATGCAAATTAACAAAagag                                     <  2:384895/139‑1 (MQ=255)                                                                                      gCAGTCATTCATCGACTCATGCCTTTCACTGATATCCCTCTCTGTTTATCATTAAt                                                                                                                       <  1:359339/56‑1 (MQ=255)                                                                                      gCAGTCATTCATCGACTCATGCCTTTCACTGATATCCCTCTCTGTTTATCATTAAt                                                                                                                       >  2:359339/1‑56 (MQ=255)                                                                                      gCAGTCATTCATCGACTCATGCCTTTCACTGATATCCCTCTCTGTTTATCATTAATTTCTAATTATCAGCGTTTTTGGCTGGCGGCGTAGCGAt                                                                                 >  1:406109/1‑94 (MQ=255)                                                                                      gCAGTCATTCATCGACTCATGCCTTTCACTGATATCCCTCTCTGTTTATCATTAATTTCTAATTATCAGCGTTTTTGGCTGGCGGCGTAGCGAt                                                                                 <  2:406109/94‑1 (MQ=255)                                                                                           cattcatCGACTCATGCCTTTCACTGATAGCCCTCTCTGTTTATCATTAATTTCTAATTATCAGCGTTTTTGGCTGGCGGCGTAGCGATGCGCTGGTTACTCTGAAAACGGTCTATGCAAATTAACAAAAGAGAATAGc                               <  1:213994/139‑1 (MQ=255)                                                                                                  cGACTCATGCCTTTCACTGATATCCCTCTCTGTTTATCATTAATTTCTAATTCTCAGCGTTTTtggctg                                                                                              >  1:567007/1‑69 (MQ=255)                                                                                                  cGACTCATGCCTTTCACTGATATCCCTCTCTGTTTATCATTAATTTCTAATTATCAGCGTTTTtggctg                                                                                              <  2:567007/69‑1 (MQ=255)                                                                                                        aTGCCTTTCACTGATATCCCTCTCTGTTTATCATTAATTTCTAATTATCAGCGTTTTtggctg                                                                                              >  2:778152/1‑63 (MQ=255)                                                                                                        aTGCCTTTCACTGATATCCCTCTCTGTTTATCATTAATTTCTAATTATCAGCGTTTTtggctg                                                                                              <  1:778152/63‑1 (MQ=255)                                                                                                        aTGCCTTTCACTGATATCCCTCTCTGTTTATCATTAATTTCTAATTATCAGCGTTTTTGGCTGGCGGCGTAGCGATgc                                                                               >  1:39974/1‑78 (MQ=255)                                                                                                        aTGCCTTTCACTGATATCCCTCTCTGTTTATCATTAATTTCTAATTATCAGCGTTTTTGGCTGGCGGCGTAGCGATgc                                                                               <  2:39974/78‑1 (MQ=255)                                                                                                            cTTTCACTGATATCCCTCTCTGTTTATCATTAATTTCTAATTATCAGCGTTTTTGGCTGGCGGCGTAGCGATGCGCTGGTTACTCTGAAAACGGTCTATGCAAATTAACAAAAGAGAATAGCTATGCATGATGCAAACa              <  2:620175/139‑1 (MQ=255)                                                                                                                    gATATCCCTCTCTGTTTATCATTAATTTCTAATTATCAGCGTTTTTGGCTGGCGGCGTAGCGATGCGCTGGTTACTCTGAAAACGGTCTATGCAAATTAACAAAAGAGAATAGCTATGCATGATGCAAACATCCGCGtt      <  2:335228/139‑1 (MQ=255)                                                                                                                     atatCCCTCTCTGTTTATCATTAATTTCTAATTATCAGCGTTTTTGGCTGGCGGCGTAg                                                                                     <  2:827899/59‑1 (MQ=255)                                                                                                                     atatCCCTCTCTGTTTATCATTAATTTCTAATTATCAGCGTTTTTGGCTGGCGGCGTAg                                                                                     >  1:827899/1‑59 (MQ=255)                                                                                                                          cctctctGTTTATCATTAATTTCTAATTATCAGCGTTTTTGGCTGGCGGCGTAGCGATGCGCTGGTTACTCTGAAAACGGTCTATGCAAATTAACAAAAGAGAATAGCTATGCATGATGCAAACATCCGCGTTGCCa  >  1:152603/1‑137 (MQ=255)                                                                                                                          cctctctGTTTATCATTAATTTCTAATTATCAGCGTTTTTGGCTGGCGGCGTAGCGATGCGCTGGTTACTCTGAAAACGGTCTATGCAAATTAACAAAAGAGAATAGCGATGCATGATGCAAACATCCGCGTTGCCa  <  2:152603/137‑1 (MQ=255)                                                                                                                              |                                                                                                                                     ATTGGATCTGGATGTGAAAGGCTTCCAGCAGTGGGTGGCTGAGGTGCTGGCTCTGGCGTCGTAACCTGTCACATGTTATTGGCATGCAGTCATTCATCGACTCATGCCTTTCACTGATATCCCTCCCTGTTTATCATTAATTTCTAATTATCAGCGTTTTTGGCTGGCGGCGTAGCGATGCGCTGGTTACTCTGAAAACGGTCTATGCAAATTAACAAAAGAGAATAGCTATGCATGATGCAAACATCCGCGTTGCCA  >  NC_000913/28144‑28401

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑