Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,471,105	G→A	pseudogene (291/291 nt) P138L (CCT→CTT)	tfaS → yfdK ←	pseudogene, CPS‑53 (KpLE1) prophage, tail fiber assembly protein fragment,Phage or Prophage Related CPS‑53 (KpLE1) prophage, conserved protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,471,105	0	G	A	100.0%	150.4 / NA	48	pseudogene (291/291 nt) P138L (CCT→CTT)	tfaS yfdK	pseudogene, CPS‑53 (KpLE1) prophage, tail fiber assembly protein fragment,Phage or Prophage Related CPS‑53 (KpLE1) prophage, conserved protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (24/24);  total (24/24)

AATTACAGGCTGGGCGGAAGCTGATGCAGGCAGAGACCTCCCGACTTAACACTGTGCTGGATTACATTGACGCGGTGACGGCAACAGATACCAGCACCGCGCCGGATGTCATCTGGCCTGAACTGCCGGAGGAGTAGGCCATTCAATATCTGGCGCACTGGAAGTATCGACCAGCTCCAGTGCGTCCAGATAATCCAGCCACAAATTATATTGCGCCAGTTCCTCACCTTTCAGACGACCAATAGCCGCTTTACCAGCCCATTGTTTAC  >  NC_000913/2470969‑2471237                                                                                                                                         |                                                                                                                                     aaTTACAGGCTGGGCGGAAGCTGATGCAGGCAGAGACCTCCCGACTTAACACTGTGCTGGATTACATTGACGCGGTGACGGCAACAGATACCAGCACCGCGCCGGATGTCATCTGGCCTGAACTGCCGGAGGAGTAAGc                                                                                                                                    <  2:32462/139‑1 (MQ=255)                   aGCTGATGCAGGCAGAGACCTCCCGACTTAACACTGTGCTGGATTACATTGACGCGGTGACGGCAACAGATACCAGCACCGCGCCGGATGTCATCTGGCCTGAACTGCCGGAGGAGTAAGCCATTCAATATCTGgcgc                                                                                                                   >  1:473370/1‑138 (MQ=255)                   aGCTGATGCAGGCAGAGACCTCCCGACTTAACACTGTGCTGGATTACATTGACGCGGTGACGGCAACAGATACCAGCACCGCGCCGGATGTCATCTGGCCTGAACTGCCGGAGGAGTAAGCCATTCAATATCTGgcgc                                                                                                                   <  2:473370/138‑1 (MQ=255)                       gATGCAGGCAGAGACCTCCCGACTTAACACTGTGCTGGATTACATTGACGCGGTGACGGCAACAGATACCAGCACCGCGCCGGATGTCATCTGGCCTGAACTGCCGGAGGAGTAAGCCATTCAATATCTGGCGCACTgg                                                                                                              >  1:448523/1‑139 (MQ=255)                             ggcagAGACCTCCCGACTTAACACTGTGCTGGATTACATTGACGCGGTGACGGCAACAGATACCAGCACCGCGCCGGATGTCATCTGGCCTGAACTGCCGGAGGAGTAAGCCATTCAATATCTGGCGCACTGGAAGTAt                                                                                                        <  2:448523/139‑1 (MQ=255)                                  agaCCTCCCGACTTAACACTGTGCTGGATTACATTGACGCGGTGACGGCAACAGATACCAGCACCGCGCCGGATGTCATCTGGCCTGAACTGCCGGAGGAGTAAGCCATTCAATATCTGGCGCACTGGAAGTATCGAcc                                                                                                   >  2:126457/1‑139 (MQ=255)                                    aCCTCCCGACTTAACACTGTGCTGGATTACATTGACGCGGTGACGGCAACAGATACCAGCACCGCGCCGGATGTCATCTGGCCTGAACTGCCGGAGGAGTAAGCCATTCAATATCTGGCGCACTGGAAGTATCGACCAg                                                                                                 >  1:652131/1‑139 (MQ=255)                                           gACTTAACACTGTGCTGGATTACATTGACGCGGTGACGGCAACAGATACCAGCACCGCGCCGGATGTCATCTGGCCTGAACTGCCGGAGGAGTAAGCCATTCAATATCTGGCGCACTGGAAGTATCGACCAGCTCCAGt                                                                                          <  2:804495/139‑1 (MQ=255)                                                       tgCTGGATTACATTGACGCGGTGACGGCAACAGATACCAGCACCGCGCCGGATGTCATCTGGCCTGAACTGCCGGAGGAGTAAGCCATTCAATATCTGGCGCACTGGAAGTATCGAc                                                                                                    <  1:807293/117‑1 (MQ=255)                                                       tgCTGGATTACATTGACGCGGTGACGGCAACAGATACCAGCACCGCGCCGGATGTCATCTGGCCTGAACTGCCGGAGGAGTAAGCCATTCAATATCTGGCGCACTGGAAGTATCGACCAGCTCCAGTGCGTCCAGATaa                                                                              >  2:29968/1‑139 (MQ=255)                                                       tgCTGGATTACATTGACGCGGTGACGGAAACAGATCCCAGCCCCGCGCCGGATGTCATCTGGCCTGAACTGCCGGAGGAGTAAGCCATTCAATATCTGGCGCACTGGAAGTATCGAc                                                                                                    >  2:807293/1‑117 (MQ=14)                                                              ttACATTGACGCGGTGACGGCAACAGATACCAGCACCGCGCCGGATGTCATCTGGCCTGAACTGCCGGAGGAGTAAGCCATTCAATATCTGGCGCACTGGAAGTATCGACCAGCTCCAGTGCGTCCAGATAATCCAGcc                                                                       <  2:134626/139‑1 (MQ=255)                                                                 cATTGACGCGGTGACGGCAACAGATACCAGCACCGCGCCGGATGTCATCTGGCCTGAACTGCCGGAGGAGTAAGCCATTCAATATCTGGCGCACTGGAAGTATCGACCAGCTCCAGTGCGTCCAGATAATCCAGcc                                                                       <  2:293308/136‑1 (MQ=255)                                                                 cATTGACGCGGTGACGGCAACAGATACCAGCACCGCGCCGGATGTCATCTGGCCTGAACTGCCGGAGGAGTAAGCCATTCAATATCTGGCGCACTGGAAGTATCGACCAGCTCCAGTGCGTCCAGATAATCCAGcc                                                                       >  1:293308/1‑136 (MQ=255)                                                                 cATTGACGCGGTGACGGCAACAGATACCAGCACCGCGCCGGATGTCATCTGGCCTGAACTGCCGGAGGAGTAAGCCATTCAATATCTGGCGCACTGGAAGTATCGACCAGCTCCAGTGCGTCCAGATAATCCAGCcaca                                                                    >  1:411576/1‑139 (MQ=255)                                                                    tGACGCGGTGACGGCAACAGATACCAGCACCGCGCCGGATGTCATCTGGCCTGAACTGCCGGAGGAGTAAGCCATTCa                                                                                                                              >  2:527819/1‑78 (MQ=21)                                                                    tGACGCGGTGACGGCAACAGATACCAGCACCGCGCCGGATGTCATCTGGCCTGAACTGCCGGAGGAGTAAGCCATTCa                                                                                                                              <  1:527819/78‑1 (MQ=21)                                                                                      aGATACCAGCACCGCGCCGGATGTCATCTGGCCTGAACTGCCGGAGGAGTAAGCCATTCAATATCTGGCGCACTGGAAGTATCGACCAGCTCCAGTGCGTCCAGATAATCCAGCCACAAATTATATTGCGCCAGTTCCt                                               <  1:29968/139‑1 (MQ=255)                                                                                          aCCAGCACCGCGCCGGATGTCATCTGGCCTGAACTGCCGGAGGAGTAAGCCATTCAATATCTGGCGCACTGGAAGTATCGACCAGCTCCAGTGCGTCCAGATAATCCAGCCACAAATTATATTGCGCCAGTTCCt                                               <  2:126805/135‑1 (MQ=255)                                                                                          aCCAGCACCGCGCCGGATGTCATCTGGCCTGAACTGCCGGAGGAGTAAGCCATTCAATATCTGGCGCACTGGAAGTATCGACCAGCTCCAGTGCGTCCAGATAATCCAGCCACAAATTATATTGCGCCAGTTCCt                                               >  1:126805/1‑135 (MQ=255)                                                                                               cACCGCGCCGGATGTCATCTGGCCTGAACTGCCGGAGGAGTAAGCCATTCAATATCTGGCGCACTGGAAGTATCGACCAGCTCCAGTGCGTCCAGATAATCCAGCCACAAATTATATTGCGCCAGTTCCTCACCTTTCa                                      >  2:318165/1‑139 (MQ=255)                                                                                               cACCGCGCCGGATGTCATCTGGCCTGAACTGCCGGAGGAGTAAGCCATTCAATATCTGGCGCACTGGAAGTATCGACCAGCTCCAGTGCGTCCAGATAATCCAGCCACAAATTATATTGCGCCAGTTCCTCACCTTTCa                                      >  2:105713/1‑139 (MQ=255)                                                                                                   gcgcCGGATGTCATCTGGCCTGAACTGCCGGAGGAGTAAGCCATTCAATATCTGGCGCACTGGAAGTATCGACCAGCTCCAGTGCGTCCAGATAATCCAGCCACAAATTATATTGCGCCAGTTCCt                                               <  2:627538/126‑1 (MQ=255)                                                                                                   gcgcCGGATGTCATCTGGCCTGAACTGCCGGAGGAGTAAGCCATTCAATATCTGGCGCACTGGAAGTATCGACCAGCTCCAGTGCGTCCAGATAATCCAGCCACAAATTATATTGCGCCAGTTACt                                               >  1:627538/1‑126 (MQ=255)                                                                                                    cgcCGGATGTCATCTGGCCTGAACTGCCGGAGGAGTAAGCCAt                                                                                                                                 <  1:634854/43‑1 (MQ=255)                                                                                                    cgcCGGATGTCATCTGGCCTGAACTGCCGGAGGAGTAAGCCAt                                                                                                                                 >  2:634854/1‑43 (MQ=255)                                                                                                    cgcCGGATGTCATCTGGCCTGAACTGCCGGAGGAGTAAGCCATTc                                                                                                                               <  2:462105/45‑1 (MQ=255)                                                                                                    cgcCGGATGTCATCTGGCCTGAACTGCCGGAGGAGTAAGCCATTc                                                                                                                               >  1:462105/1‑45 (MQ=255)                                                                                                    cgcCGGATGTCATCTGGCCTGAACTGCCGGAGGAGTAAGCCATTCAATATCTGGCGCACTGGAAGTAt                                                                                                        >  2:841955/1‑68 (MQ=255)                                                                                                    cgcCGGATGTCATCTGGCCTGAACTGCCGGAGGAGTAAGCCATTCAATATCTGGCGCACTGGAAGTAt                                                                                                        <  1:841955/68‑1 (MQ=255)                                                                                                    cgcCGGATGTCATCTGGCCTGAACTGCCGGAGGAGTAAGCCATTCAATATCTGGCGCACTGGAAGTATCGACCAGCTCCAg                                                                                           <  1:823151/81‑1 (MQ=255)                                                                                                    cgcCGGATGTCATCTGGCCTGAACTGCCGGAGGAGTAAGCCATTCAATATCTGGCGCACTGGAAGTATCGACCAGCTCCAg                                                                                           >  2:823151/1‑81 (MQ=255)                                                                                                    cgcCGGATGTCATCTGGCCTGAACTGCCGGAGGAGTAAGCCATTCAATATCTGGCGCACTGGAAGTATCGACCAGCTCCAGTGCGTCCAGATAATCCAGCCACAAATTATATTGCGCCAGTTCCTCACCTTTCAgacga                                 >  1:658285/1‑139 (MQ=255)                                                                                                    cgcCGGATGTCATCTGGCCTGAACTGCCGGAGGAGTAAGCCATTCAATATCTGGCGCACTGGAAGTATCGACCAGCTCCAGTGCGTCCAGATAATCCAGCCACAAATTATATTGCGCCAGTTCCTCACCTTTCAgacga                                 >  2:60364/1‑139 (MQ=255)                                                                                                               aTCTGGCCTGAACTGCCGGAGGAGTAAGCCATTCAATATCTGGCGCACTGGAAGTATCGACCAGCTCCAGTGCGTCCAGATAATCCAGCCACAAATTATATTGCGCCAGTTCCTCACCTTTCAGACGACCAATAGCCGc                      >  2:628654/1‑139 (MQ=255)                                                                                                               aTCTGGCCTGAACTGCCGGAGGAGTAAGCCATTCAATATCTGGCGCACTGGAAGTATCGACCAGCTCCAGTGCGTCCAGATAATCCAGCCACAAATTATATTGCGCCAGTTCCTCACCTTTCAGACGACCAATAGCCGc                      >  2:533818/1‑139 (MQ=255)                                                                                                                 cTGGCCTGAACTGCCGGAGGAGTAAGCCATTCAATATCTGGCGCACTGGAAGTATCGACCAGCTCCAGTGCGTCCAGATAATCCAGCCACAAATTATATTGCGCCAGTTCCTCACCTTTCAGACGACCAATAGCCGCtt                    <  1:533818/139‑1 (MQ=255)                                                                                                                 cTGGCCTGAACTGCCGGAGGAGTAAGCCATTCAATATCTGGCGCACTGGAAGTATCGACCAGCTCCAGTGCGTCCAGATAATCCAGCCACAAATTATATTGCGCCAGTTCCTCACCTTTCAGACGACCAATAGCCGCtt                    <  1:628654/139‑1 (MQ=255)                                                                                                                     ccTGAACTGCCGGAGGAGTAAGCCATTCAATATCTGGCGCACTGGAAGTATCGACCAGCTCCAGTGCGTCCAGATAATCCAGCCACAAATTATATTGCGCCAGTTCCTCACCTTTCAGACGACCAATAGCCGCTTTAcc                >  1:67055/1‑139 (MQ=255)                                                                                                                        gAACTGCCGGAGGAGTAAGCCATTCAATATCTGGCGCACTGGAAGTATCGACCAGCTCCAGTGCGTCCAGATAATCCAGCCACAAATTATATTGCGCCAGTTCCTCACCTTTCAGACGACCAATAGCCGCTTTACCAGc             <  1:60364/139‑1 (MQ=255)                                                                                                                        gAACTGCCGGAGGAGTAAGCCATTCAATATCTGGCGCACTGGAAGTATCGACCAGCTCCAGTGCGTCCAGATAATCCAGCCACAAATTATATTGCGCCAGTTCCTCACCTTTCAGACGACCAATAGCCGCTTTACCAGc             <  1:105713/139‑1 (MQ=255)                                                                                                                         aaCTGCCGGAGGAGTAAGCCATTCAATATCTGGCGCACTGGAAGTATCGACCAGCTCCAGTGCGTCCAGATAATCCAGCCACAAATTATATTGCGCCAGTTCCTCACCTTTCAGACGACCAATAGCCGCTTTACCAGcc            <  1:515343/139‑1 (MQ=255)                                                                                                                          aCTGCCGGAGGAGTAAGCCATTCAATATCTGGCGCACTGGAAGTATCGACCAGCTCCAGTGCGTCCAGATAATCCAGCCACAAATTATATTGCGCCAGTTCCTCACCTTTCAGACGACCAATAGCCGCTTTACCAGccc           <  1:126457/139‑1 (MQ=255)                                                                                                                              ccGGAGGAGTAAGCCATTCAATATCTGGCGCACTGGAAGTATCGACCAGCTCCAGTGCGTCCAGATAATCCAg                                                                         <  2:777465/73‑1 (MQ=255)                                                                                                                              ccGGAGGAGTAAGCCATTCAATATCTGGCGCACTGGAAGTATCGACCAGCTCCAGTGCGTCCAGATAATCCAg                                                                         >  1:777465/1‑73 (MQ=255)                                                                                                                              ccGGAGGAGTAAGCCATTCAATATCTGGCGCACTGGAAGTATCGACCAGCTCCAGTGCGTCCAGATAATCCAGCCACAAATTATATTGCGCCAGTTCCTCACCTTTCAGACGACCAATAGCCGCTTTACCAGCCCATTg       >  1:157990/1‑139 (MQ=255)                                                                                                                                  aggagTAAGCCATTCAATATCTGGCGCACTGGAAGTATCGACCAGCTCCAGTGCGTCCAGATAATCCAGCCACAAATTATATTGCGCCAGTTCCTCACCTTTCAGACGACCAATAGCCGCTTTACCAGCCCATTGTTTa   <  2:658285/139‑1 (MQ=255)                                                                                                                                   ggagTAAGCCATTCAATATCTGGCGCACTGGAAGTATCGACCAGCTCCAGTGCGTCCAGATAATCCAGCCACAAATTATATTGCGCCAGTTCCTCACCTTTCAGACGACCAATAGCCGCTTTACCAGCCCATTGTTTAc  <  2:67055/139‑1 (MQ=255)                                                                                                                                         |                                                                                                                                     AATTACAGGCTGGGCGGAAGCTGATGCAGGCAGAGACCTCCCGACTTAACACTGTGCTGGATTACATTGACGCGGTGACGGCAACAGATACCAGCACCGCGCCGGATGTCATCTGGCCTGAACTGCCGGAGGAGTAGGCCATTCAATATCTGGCGCACTGGAAGTATCGACCAGCTCCAGTGCGTCCAGATAATCCAGCCACAAATTATATTGCGCCAGTTCCTCACCTTTCAGACGACCAATAGCCGCTTTACCAGCCCATTGTTTAC  >  NC_000913/2470969‑2471237

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑