Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,552,094	G→A	I15I (ATC→ATT)	ypeA ←	GNAT family putative N‑acetyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,552,094	0	G	A	100.0%	84.5 / NA	27	I15I (ATC→ATT)	ypeA	GNAT family putative N‑acetyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (14/13);  total (14/13)

CATCACCGTTCCGACCACGTCACCGTTTACCTCAGCGACCAAAAACAAACTGACGTCATGGTTCATCTTACGCTCGATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTGATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCTCCATGGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGAACGG  >  NC_000913/2551962‑2552188                                                                                                                                     |                                                                                               cATCACCGTTCCGACCACGTCACCGTTTACCTCAGCGACCAAAAACAAACTGACGTCATGGTTCATCTTACGCTCGATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGAcc                                                                                          >  2:359770/1‑139 (MQ=255) cATCACCGTTCCGACCACGTCACCGTTTACCTCAGCGACCAAAAACAAACTGACGTCATGGTTCATCTTACGCTCGATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGAcc                                                                                          >  1:711361/1‑139 (MQ=255)              aCCACGTCACCGTTTACCTCAGCGACCAAAAACAAACTGACGTCATGGTTCATCTTACGCTCGATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCtt                                                                             >  2:219997/1‑139 (MQ=255)              aCCACGTCACCGTTTACCTCAGCGACCAAAAACAAACTGACGTCATGGTTCATCTTACGCTCGATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCtt                                                                             >  1:330162/1‑139 (MQ=255)              aCCACGTCACCGTTTACCTCAGCGACCAAAAACAAACTGACGTCATGGTTCATCTTACGCTCGATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCtt                                                                             >  2:462609/1‑139 (MQ=255)               ccACGTCACCGTTTACCTCAGCGACCAAAAACAAACTGACGTCATGGTTCATCTTACGCTCGATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTc                                                                            <  1:219997/139‑1 (MQ=255)                     cACCGTTTACCTCAGCGACCAAAAACAAACTGACGTCATGGTTCATCTTACGCTCGATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCg                                                                      >  1:556876/1‑139 (MQ=255)                            tACCTCAGCGACCAAAAACAAACTGACGTCATGGTTCATCTTACGCTCGATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATAcg                                                               <  2:711361/139‑1 (MQ=255)                             aCCTCAGCGACCAAAAACAAACTGACGTCATGGTTCATCTTACGCTCGATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATAcgc                                                              >  2:404649/1‑139 (MQ=255)                               ctgaGCGACCAAAAACAAACTGACGTCATGGTTCATCTTACGCTCGATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGt                                                            <  2:556876/136‑1 (MQ=255)                                    cGACCAAAAACAAACTGACGTCATGGTTCATCTTACGCTCGATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTAtctc                                                       >  2:705470/1‑139 (MQ=255)                                                                     tACGCTCGATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCTCCATGGTATTCGTTACCTTTTTGCGGGTTAAAAg                      <  1:359770/139‑1 (MQ=255)                                                                      aCGCTCGATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCTCCATGGTATTCGTTACCTTTTTGCGGGTTAAAAgg                     <  1:705470/139‑1 (MQ=255)                                                                       cGCTCGATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGAcc                                                                                          >  1:382126/1‑69 (MQ=255)                                                                       cGCTCGATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGAcc                                                                                          <  2:382126/69‑1 (MQ=255)                                                                       cGCTCGATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCTCCATGGTATTCGTTACCTTTTTGCGGGTTAAAAGGc                    <  2:330162/139‑1 (MQ=255)                                                                           cGATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATAcgcg                                                             <  2:452581/94‑1 (MQ=255)                                                                           cGATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATAcgcg                                                             >  1:222320/1‑94 (MQ=255)                                                                           cGATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATAcgcg                                                             <  2:222320/94‑1 (MQ=255)                                                                           cGATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATAcgcg                                                             >  1:452581/1‑94 (MQ=255)                                                                            gATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCTCCATGGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGAtt               >  1:238684/1‑139 (MQ=255)                                                                                         ggATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCTCCATGGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGAACgg  <  2:238684/139‑1 (MQ=255)                                                                                         ggATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCTCCATGGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGAACgg  <  1:365054/139‑1 (MQ=255)                                                                                                             caAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATAcgcg                                                             <  1:821433/60‑1 (MQ=255)                                                                                                             caAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATAcgcg                                                             >  2:821433/1‑60 (MQ=255)                                                                                                                 tCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCTCCATGGTa                                                >  1:766684/1‑69 (MQ=255)                                                                                                                 tCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCTCCATGGTa                                                <  2:766684/69‑1 (MQ=255)                                                                                                                                     |                                                                                               CATCACCGTTCCGACCACGTCACCGTTTACCTCAGCGACCAAAAACAAACTGACGTCATGGTTCATCTTACGCTCGATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTGATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCTCCATGGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGAACGG  >  NC_000913/2551962‑2552188

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑