Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 253,084 A→G T126A (ACA→GCA)  yafP → GNAT family putative N‑acetyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913253,0840AG96.4% 91.6 / ‑6.0 28T126A (ACA→GCA) yafPGNAT family putative N‑acetyltransferase
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (13/14);  total (14/14)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTATTTGTTGACCCTGAATACACCCGCCGTGGGGTTGCCAGCGCTTTGTTAAAACCTTTGATTAAGTCTGAATCCGAACTTACGGTGGACGCAAGCATAACCGCAAAACCCTTTTTTGAACGTTATGGTTTTCAGACAGTTAAGCAGCAGCGCGTTGAATGCCGGGGAGCGTGGTTTACTAATTTTTATATGCGATATAAACCGCAACATTAAAT  >  NC_000913/252949‑253163
                                                                                                                                       |                                                                               
ttATTTGTTGACCCTGAATACACCCGCCGTGGGGTTGCCAGCGCTTTGTTAAAACCTTTGATTAAGTCTGAGTCCGAACTTACGGTGGACGCAAGCATAACCGCAAAACCCTTTTTTGAACGTTATGGTTTTCAGGCAg                                                                              <  1:27246/139‑1 (MQ=255)
ttATTTGTTGACCCTGAATACACCCGCCGTGGGGTTGCCAGCGCTTTGTTAAAACCTTTGATTAAGTCTGAATCCGAACTTACGGTGGACGCAAGCATAACCGCAAAACCCTTTTTTGAACGTTATGGTTTTCAGGCAg                                                                              <  1:136683/139‑1 (MQ=255)
  aTTTGTTGACCCTGAATACACCCGCCGTGGGGTTGCCAGCGCTTTGTTAAAACCTTTGATTAAGTCTGAATCCGAACTTACGGTGGACGCAAGCATAACCGCAAAACCCTTTTTTGAACGTTATGGTTTTCAGGCAGtt                                                                            >  1:354403/1‑139 (MQ=255)
   cttgttgCCCCTGAATACACCCGCCGTGGGGTTGCCAGCTCTTTGTTAAAACCTTTGATTAAGTCTGAATCTGAACTTACGGTGGACGCAAGCATAACCGCAAAACCCTTTTTTGAACGTTATGGTTTTCAGGCAGTTa                                                                           >  2:169478/2‑139 (MQ=255)
     tgttgACCCTGAATACACCCGCCGTGGGGTTGCCAGCGCTTTGTTAAAACCTTTGATTAAGTCTGAATCCGAACTTACGGTGGACGCAAGCATAACCGCAAAACCCTTTTTTGAACGTTATGGTTTTCAGGCAg                                                                              >  2:674604/1‑134 (MQ=255)
     tgttgACCCTGAATACACCCGCCGTGGGGTTGCCAGCGCTTTGTTAAAACCTTTGATTAAGTCTGAATCCGAACTTACGGTGGACGCAAGCATAACCGCAAAACCCTTTTTTGAACGTTATGGTTTTCAGGCAg                                                                              <  1:674604/134‑1 (MQ=255)
          aCCCTGAATACACCCGCCGTGGGGTTGCCAGCGCTTTGTTAAAACCTTTGATTAAGTCTGAATCCGAACTTACGGTGGACGCAAGCATAACCGCAAAACCCTTTTTTGAACGTTATGGTTTTCAGGCAGTTAagcagca                                                                    >  2:614201/1‑139 (MQ=255)
                     acCCGCCGTGGGGTTGCCAGCGCTTTGTTAAAACCTTTGATTAAGTCTGAATCCGAACTTACGGTGGACGCAAGCATAACCGCAAAACCCTTTTTTGAACGTTATGGTTTTCAGGCAGTTAag                                                                         <  1:190934/123‑1 (MQ=255)
                     acCCGCCGTGGGGTTGCCAGCGCTTTGTTAAAACCTTTGATTAAGTCTGAATCCGAACTTACGGTGGACGCAAGCATAACCGCAAAACCCTTTTTTGAACGTTATGGTTTTCAGGCAGTTAag                                                                         >  2:190934/1‑123 (MQ=255)
                     acCCGCCGTGGGGTTGCCAGCGCTTTGTTAAAACCTTTGATTAAGTCTGAATCCGAACTTACGGTGGACGCAAGCATAACCGCAAAACCCTTTTTTGAACGTTATGGTTTTCAGGCAGTTAAGCAGCAGCGCGTTGAAt                                                         >  1:312205/1‑139 (MQ=255)
                      cccGCCGTGGGGTTGCCAGCGCTTTGTGAAAACCTTTGATTAAGTCTGAATCCGAACTTACGGTGGACGCAAGCATAACCGCAAAACCCTTTTTTGAACGTTATGGTTTTCAGACAGTTAAGCAGCATCGCGTTGAATg                                                        >  1:542630/1‑139 (MQ=255)
                          ccgTGGGGTTGCCAGCGCTTTGTTAAAACCTTTGATTAAGTCTGAATCCGAACTTACGGTGGACGCAAGCATAACCGCAAAACCCTTTTTTGAACGTTATGGTTTTCAGGCAGTTAAGCAGCAGCGCGTTGAATGcccg                                                    >  2:172254/1‑137 (MQ=255)
                          ccgTGGGGTTGCCAGCGCTTTGTTAAAACCTTTGATTAAGTCTGAATCCGAACTTACGGAGGACGCAAGCATAACCGCAAAACCCTTTTTTGAACGTTATGGTTTTCAGGCAGTTAATCAGCAGCGCGTTGAATGCCgg                                                    >  2:543665/1‑139 (MQ=255)
                                ggTTGCCAGCGCTTTGTTAAAACCTTTGATTAAGTCTGAATCCGAACTTACGGTGGACGCAAGCATAACCGCAAAACCCTTTTTTGAACGTTATGGTTTTCAGGCAGTTAAGCAGCAGCGCGTTGAATGCCGGGGAGCg                                              <  2:312205/139‑1 (MQ=255)
                                                  aaaaCCTTTGATTAAGTCTGAATCCGAACTTACGGTGGACGCAAGCATAACCGCAAAACCCTTTTTTGAACGTTATGGTTTTCAGGCAGTTAGGCAGCAGCGCGTTGAATGCCGGGGAGCGTGGTTTACTAATTTTtat                            <  1:169478/139‑1 (MQ=255)
                                                     aCCTTTGATTAAGTCTGAATCCGAACTTACGGTGGACGCAAGCATAACCGCAAAACCCTTTTTTGAACGTTATGGTTTTCAGGCAGTTAAGCAGCAGCGCGTTGAATGCCCGGGAGCGTGGTTTACTAATTTTTATATg                         <  1:172254/139‑1 (MQ=255)
                                                      ccTTTGATTAAGTCTGAATCCGAACTTACGGTGGACGCAAGCATAACCGCAAAACCCTTTTTTGAACGTTATGGTTTTCAGGCAGTTAAGCAGCAGCGCGTTGAATGCCGGGGAGCGTGGTTTACTAATTTTTATATGc                        <  2:811698/139‑1 (MQ=255)
                                                                aGTCTGAATCCGAACTTACGGTGGACGCAAGCATAACCGCAAAACCCTTTTTTGAACGTTATGGTTTTCAGGCAGTTAagcag                                                                      >  2:144639/1‑83 (MQ=255)
                                                                aGTCTGAATCCGAACTTACGGTGGACGCAAGCATAACCGCAAAACCCTTTTTTGAACGTTATGGTTTTCAGGCAGTTAagcag                                                                      <  1:144639/83‑1 (MQ=255)
                                                                   cTGAATCCGAACTTACGGTGGACGAAAGCATAACCGCAAAACCCTTTTTTGAACGTTATGGTTTTCAGGCAGTTAAGCAGCAGCGCGTTGAATGCCGGGGAGCGTGGTTTACTAATTTTTATATGCGATATAAACCGCa           <  1:468024/139‑1 (MQ=255)
                                                                      aaTCCGAACTTACGGTGGACGCAAGCATAACCGCAAAACCCTTTTTTGAACGTTATGGTTTTCAGGCAGTTAAGCAGCAGCGCGTTGAATGCCGGGGAGCGTGGTTTACTAATTTTTATATGCGATATAAACCg             >  2:240127/1‑134 (MQ=255)
                                                                      aaTCCGAACTTACGGTGGACGCAAGCATAACCGCAAAACCCTTTTTTGAACGTTATGGTTTTCAGGCAGTTAAGCAGCAGCGCGTTGAATGCCGGGGAGCGTGGTTTACTAATTTTTATATGCGATATAAACCg             <  1:240127/134‑1 (MQ=255)
                                                                       aTCCGAACTTACGGTGGACGCAAGCATAACCGCAAAACCCTTTTTTGAACGTTATGGTTTTCAGGCAGTTAAGCAGCAGCGCGTTGAATGCCGGGGAGCGTGGTTTACTAATTTTTATATGCGATATAAACCGCAACAt       <  1:614201/139‑1 (MQ=255)
                                                                            aaCTTACGGTGGACGCAAGCATAACCGCAAAACCCTTTTTTGGACGTTATGGTTTTCAGGCAGTTAAGCAGCAGCGCGTTGAATGCCGGGGAGCGTGGTTTACTAATTTTTATATGCGATATAAACCGCAACATTAAAt  >  1:548393/1‑139 (MQ=255)
                                                                                               cATAACCGCAAAACCCTTTTTTGAACGTTATGGTTTTCAGGCAGTTAAGCAGCAGCGCGTTGAATGCCGGGGAGCGTGGTTTACTAATTTTTATATGCGATATAAACCGc            <  2:23227/110‑1 (MQ=255)
                                                                                               cATAACCGCAAAACCCTTTTTTGAACGTTATGGTTTTCAGGCAGTTAAGCAGCAGCGCGTTGAATGCCGGGGAGCGTGGTTTACTAATTTTTATATGCGATATAAACCGc            >  1:23227/1‑110 (MQ=255)
                                                                                                 tAACCGCAAAACCCTTTTTTGAACGTTATGGTTTTCAGGCAGTTAAGCAGCAGCGCGTTGAATGCCGGGGAGCGTGGTTTACTAATTTTTatat                          <  1:798533/94‑1 (MQ=255)
                                                                                                 tAACCGCAAAACCCTTTTTTGAACGTTATGGTTTTCAGGCAGTTAAGCAGCAGCGCGTTGAATGCCGGGGAGCGTGGTTTACTAATTTTTatat                          >  2:798533/1‑94 (MQ=255)
                                                                                                                                       |                                                                               
TTATTTGTTGACCCTGAATACACCCGCCGTGGGGTTGCCAGCGCTTTGTTAAAACCTTTGATTAAGTCTGAATCCGAACTTACGGTGGACGCAAGCATAACCGCAAAACCCTTTTTTGAACGTTATGGTTTTCAGACAGTTAAGCAGCAGCGCGTTGAATGCCGGGGAGCGTGGTTTACTAATTTTTATATGCGATATAAACCGCAACATTAAAT  >  NC_000913/252949‑253163

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: