Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,629,481 G→A *64* (TAG→TAA yfgG → uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,629,4810GA100.0% 93.2 / NA 30*64* (TAG→TAAyfgGuncharacterized protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (13/17);  total (13/17)

CTCATCAGCTTTATCTTCTTCTTTGGCCGTTTTATCTACTCGTCCGTCGGTGCCTGGCAGCACCATCAGAGCAAAAAAGAAGCTCAGCAATCCACACTCTCCGTCGAATCACCGGTACAACGTTAGCGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTGCTGTTTGGGATTATCAAAGCGGCAGATATTCTTTCATCTTAAATTTTACGTCTTTATCCTGACTGATGTTTATCCTGT  >  NC_000913/2629356‑2629616
                                                                                                                             |                                                                                                                                       
cTCATCAGCTTTATCTTCTTCTTTGGCCGTTTTATCTACTCGTCCGTCGGTGCCTGGCAGCACCATCAGAGCAAAAAAGAAGCTCAGCAATCCACACTCTCCGTCGAATCACCGGTACAACGTTAACGGTTACCTtctc                                                                                                                            >  1:103626/1‑139 (MQ=255)
   atcaGCTTTATCTTCTTCTTTGGCCGTTTTATCTACTCGTCCGTCGGTGCCTGGCAGCACCATCAGAGCAAAAAAGAAGCTCAGCAATCCACACTCTCCGTCGAATCACCGGTACAACGTTAACGGTTACCTTCTCCAc                                                                                                                         <  2:103626/139‑1 (MQ=255)
                                    tACTCGTCCGTCGGTGCCTGGCAGCACCATCAGAGCAAAAAAGAAGCTCAGCAATCCACACTCTCCGTCGAATCACCGGTACAACGTTAACGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGa                                                                                        <  2:528289/139‑1 (MQ=255)
                                         gtccgtcGGTGCCTGGCAGCACCATCAGAGCAAAAAAGAAGCTCAGCAATCCACACTCTCCGTCGAATCACCGGTACAACGTTAACGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCtt                                                                                   >  1:160561/1‑139 (MQ=255)
                                         gtccgtcGGTGCCTGGCAGCACCATCAGAGCAAAAAAGAAGCTCAGCAATCCACACTCTCCGTCGAATCACCGGTACAACGTTAACGGTTACCTTCTCCACTTTCACAGAACATAACGGAACTTCGCTGTCGGa                                                                                        >  2:548056/1‑134 (MQ=255)
                                         gtccgtcGGTGCCTGGCAGCACCATCAGAGCAAAAAAGAAGCTCAGCAATCCACACTCTCCGTCGAATCACCGGTACAACGTTAACGGTTAACTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGa                                                                                        <  1:548056/134‑1 (MQ=255)
                                            cgtcGGTGCCTGGCAGCACCATCAGAGCAAAAAAGAAGCTCAGCAATCCACACTCTCCGTCGAATCACCGGTACAACGTTAACGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTg                                                                                <  1:800717/139‑1 (MQ=255)
                                              tcGGTGCCTGGCAGCACCATCAGAGCAAAAAAGAAGCTCAGCAATCCACACTCTCCGTCGAATCACCGGTACAACGTTAACGGTTACCTTCTCCAc                                                                                                                         >  2:816950/1‑96 (MQ=255)
                                              tcGGTGCCTGGCAGCACCATCAGAGCAAAAAAGAAGCTCAGCAATCCACACTCTCCGTCGAATCACCGGTACAACGTTAACGGTTACCTTCTCCAc                                                                                                                         <  1:816950/96‑1 (MQ=255)
                                              tcGGTGCCTGGCAGCACCATCAGAGCAAAAAAGAAGCTCAGCAATCCACACTCTCCGTCGAATCACCGGTACAACGTTAACGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTGCt                                                                              >  2:721099/1‑139 (MQ=255)
                                                ggTGCCTGGCAGCACCATCAGAGCAAAAAAGAAGCTCAGCAATCCACACTCTCCGTCGAATCACCGGTACAACGTTAACGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTGCTGt                                                                            <  2:93567/139‑1 (MQ=255)
                                                ggTGCCTGGCAGCACCATCAGAGCAAAAAAGAAGCTCAGCAATCCACACTCTCCGTCGAATCACCGGTACAACGTTAACGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTGCTGt                                                                            <  1:539043/139‑1 (MQ=255)
                                                                  cAGAGCAAAAAAGAAGCTCAGCAATCCACACTCTCCGTCGAATCACCGGTACAACGTTAACGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTGCTGTTTGGGATTATCAAAGCgg                                                          <  1:290767/139‑1 (MQ=255)
                                                                   agagCAAAAAAGAAGCTCAGCAATCCACACTCTCCGTCGAATCACCGGTACAACGTTAACGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTGCTGTTTGGGATTATCAAAGCGGc                                                         <  1:721099/139‑1 (MQ=255)
                                                                        aaaaaaGAAGCTCAGCAATCCACACTCTCCGTCGAATCACCGGTACAACGTTAACGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTGCTGTTTGGGATTATCAAAGCGGCAGata                                                    <  2:160561/139‑1 (MQ=255)
                                                                                            cacaCTCTCCGTCGAATCACCGGTACTACGTTAACCGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTGCTGTTTGGGATGATCAAAGCGGCAGATATTCTTTCATCTTAAATTTTa                                <  2:162051/139‑1 (MQ=255)
                                                                                                   tcCGTCGAATCACCGGTACAACGTTAACGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCtttt                                                                                 >  1:803590/1‑83 (MQ=255)
                                                                                                   tcCGTCGAATCACCGGTACAACGTTAACGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCtttt                                                                                 <  2:803590/83‑1 (MQ=255)
                                                                                                    ccGTCGAATCACCGGTACAACGTTAACGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTGCTGTTTGGGATTATCAAAGCGGCAGATATTCTTTCATCTTAAATTTTACGTCTTTa                        >  2:208647/1‑139 (MQ=255)
                                                                                                     cGTCGAATCACCGGTACAACGTTAACGGTTACCTTCTCCACtt                                                                                                                       >  2:783314/1‑43 (MQ=255)
                                                                                                     cGTCGAATCACCGGTACAACGTTAACGGTTACCTTCTCCACtt                                                                                                                       <  1:783314/43‑1 (MQ=255)
                                                                                                     cGTCGAATCACCGGTACAACGTTAACGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTGCTGTTTGGGATTATCAAAGCGGCAGATATTCt                                                <  2:323207/114‑1 (MQ=255)
                                                                                                     cGTCGAATCACCGGTACAACGTTAACGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTGCTGTTTGGGATTATCAAAGCGGCAGATATTCt                                                >  1:323207/1‑114 (MQ=255)
                                                                                                           aTCACCGGTACAACGTTAACGGTTACCTTCTCCACTTTca                                                                                                                    >  2:65657/1‑40 (MQ=255)
                                                                                                           aTCACCGGTACAACGTTAACGGTTACCTTCTCCACTTTca                                                                                                                    <  1:65657/40‑1 (MQ=255)
                                                                                                             cACCGGTACAACGTTAACGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTGCTGTTTGGGATTATCAAAGCGGCAGATATTCTTTCATCTTAAATTTTACGTCTTTATCctgactg               >  1:376360/1‑139 (MQ=255)
                                                                                                               ccGGTACAACGTTAACGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTGCTGTTTGGGATTATCAAAGCGGCAGATATTCTTTCATCTTAAATTTTACGTCTTTATCCTGACTGAt             <  2:376360/139‑1 (MQ=255)
                                                                                                                        cGTTAACGGTTACCTTCTCGACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTGCTGTTTGGGATTATCAAAGc                                                            <  2:546179/83‑1 (MQ=255)
                                                                                                                        cGTTAACGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTGCTGTTTGGGATTATCAAAGc                                                            >  1:546179/1‑83 (MQ=255)
                                                                                                                          ttAACGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTGCTGTTTGGGATTATCAAAGCGGCAGATATTCTTTCATCTTAAATTTTACGTCTTTATCCTGACTGATGTTTATCCTGt  >  2:139688/1‑139 (MQ=255)
                                                                                                                             |                                                                                                                                       
CTCATCAGCTTTATCTTCTTCTTTGGCCGTTTTATCTACTCGTCCGTCGGTGCCTGGCAGCACCATCAGAGCAAAAAAGAAGCTCAGCAATCCACACTCTCCGTCGAATCACCGGTACAACGTTAGCGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTGCTGTTTGGGATTATCAAAGCGGCAGATATTCTTTCATCTTAAATTTTACGTCTTTATCCTGACTGATGTTTATCCTGT  >  NC_000913/2629356‑2629616

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: