Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,645,013	C→T	*771* (TAG→TAA)	pbpC ←	penicillin‑insensitive murein repair transglycosylase, inactive transpeptidase domain protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,645,013	0	C	T	100.0%	69.2 / NA	23	*771* (TAG→TAA)	pbpC	penicillin‑insensitive murein repair transglycosylase, inactive transpeptidase domain protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (11/12);  total (11/12)

GATGGCGCGGATTATAATGAGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAATTGATAATCACCTTTATCCGTCAAATGCAGGGTCACGTTGCGCCCGCGTTCAGTTAACGGTTCGCCATTCAGA  >  NC_000913/2644911‑2645142                                                                                                       |                                                                                                                                  gaTGGCGCGGATTATAATGAGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGATTATTGCATGACAAATTTCACTGTCGCGATTTGcccc                                                                                               >  1:2317/1‑139 (MQ=255) gaTGGCGCGGATTATAATGAGCAACAGGGCCGTTGACTATTGATG‑AGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGATTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCa                                                                                              <  1:730344/139‑1 (MQ=255)   tGGCGCGGATTATAATGAGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGATTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCAc                                                                                             >  1:383689/1‑139 (MQ=255)                  tGAGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGATTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAg                                                                              <  2:383689/139‑1 (MQ=255)                            ggCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGATTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAATTGATaa                                                                    <  2:2317/139‑1 (MQ=255)                             gCCGTTGACTATTGATGAATGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACTGATTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAATTGATAAt                                                                   <  2:368034/139‑1 (MQ=255)                                      tATTGATGAAGGTAACATTTCTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGATTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAATTGATAATCACCtttct                                                          >  2:493735/1‑137 (MQ=255)                                              aaGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGATTATTGCATGACAAATTTCACTGTCGCGATTTGcc                                                                                                 >  2:637849/1‑92 (MQ=255)                                              aaGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGATTATTGCATGACAAATTTCACTGTCGCGATTTGcc                                                                                                 <  1:637849/92‑1 (MQ=255)                                                     cATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGATTATTGCATGACAAATTTCACTGTCGCGa                                                                                                       <  2:782214/79‑1 (MQ=255)                                                     cATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGATTATTGCATGACAAATTTCACTGTCGCGa                                                                                                       >  1:782214/1‑79 (MQ=255)                                                     cATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGATTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCa                                                                                              <  2:511933/88‑1 (MQ=255)                                                     cATTTTTTTAAAATAAAATGAGTTGTAGCAACAATCAGGGACAAAACAGATTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCa                                                                                              >  1:511933/1‑88 (MQ=255)                                                                     aatGAGTTTTAGCAACAATCAGGGACAAAACAGATTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAATTGATAATCACCTTTATCCGTc                                                     >  2:440333/1‑113 (MQ=255)                                                                     aatGAGTTTTAGCAACAATCAGGGACAAAACAGATTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAATTGATAATCACCTTTATCCGTc                                                     <  1:440333/113‑1 (MQ=255)                                                                             ttaacaacaaTCAGGGACAAAACAGATTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAATTGATAATCACCTTTATCCGTCAAATGCAGGGTCACGTTGCGCCCGCGTTCAGTTa                   >  2:284127/5‑139 (MQ=255)                                                                               aGCAACAATCAGGGACAAAACAGATTATTGCATGACAAATTTCACTGTCGCGATTTGcccc                                                                                               <  2:222179/61‑1 (MQ=255)                                                                               aGCAACAATCAGGGACAAAACAGATTATTGCATGACAAATTTCACTGTCGCGATTTGcccc                                                                                               >  1:222179/1‑61 (MQ=255)                                                                                           ggACAAAACAGATTATTGCATGACAAATTTCACTGTCGCGATTTGccc                                                                                                >  1:575999/1‑48 (MQ=255)                                                                                           ggACAAAACAGATTATTGCATGACAAATTTCACTGTCGCGATTTGccc                                                                                                <  2:575999/48‑1 (MQ=255)                                                                                              cAAAACAGATTATTGCATGACTAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAATTGATAATCACCTTTATCCGTCAAATGCAGGGTCACGTTGCGCCCGCGTTCAGTTAACGGTTCGCCATTCAGa  <  1:493735/139‑1 (MQ=255)                                                                                                aaaCAGATTATTGCATGACAAATTTCACTGTCGCGAt                                                                                                      <  1:685074/37‑1 (MQ=255)                                                                                                aaaCAGATTATTGCATGACAAATTTCACTGTCGCGAt                                                                                                      >  2:685074/1‑37 (MQ=255)                                                                                                       |                                                                                                                                  GATGGCGCGGATTATAATGAGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAATTGATAATCACCTTTATCCGTCAAATGCAGGGTCACGTTGCGCCCGCGTTCAGTTAACGGTTCGCCATTCAGA  >  NC_000913/2644911‑2645142

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑