Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,800,475 G→A *110* (TAG→TAA ygaM → putative membrane‑anchored DUF883 family ribosome‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,800,4750GA100.0% 96.3 / NA 31*110* (TAG→TAAygaMputative membrane‑anchored DUF883 family ribosome‑binding protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (16/15);  total (16/15)

GTCCAGCAAGCCGCGCGCGATGCCGTTGGCTGCGCGGATTCTTTTGTTCGTGAAAGACCCTGGTGTAGCGTGGGTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTATT  >  NC_000913/2800344‑2800603
                                                                                                                                   |                                                                                                                                
gTCCAGCAAGCCGCGCGCGATGCCGTTGGCTGCGCGGATTCTTTTGTTCGTGAAAGACCCTGGTGTAGCGTGGGTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCaaaa                                                                                                                           <  1:74695/139‑1 (MQ=255)
  ccAGCAAGCCGAGCGCGATGCCGTTGGCTGCGCGGATTCTTTTGTTCGTGAAAGACCCTGGTGTAGCGTGGGTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAAt                                                                                                                         <  1:54789/139‑1 (MQ=255)
                            gCTGCGCGGATTCTTTTGTTCGTGAAAGACCCTGGTGTAGCGTGGGTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGAcccc                                                                                               >  2:622870/1‑139 (MQ=255)
                              tGCGCGGATTCTTTTGTTCGTGAAAGACCCTGGTGTAGCGTGGGTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGACCCCGa                                                                                             >  1:436321/1‑139 (MQ=255)
                                                                   gCGTGGGTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATca                                                        >  1:367622/1‑139 (MQ=255)
                                                                         gTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGTGTGTTTGACCCCGAAGCCTGTCTTCGGGGTTTCtttt                                                                      <  2:85469/119‑1 (MQ=255)
                                                                         gTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCtttt                                                                      >  1:85469/1‑119 (MQ=255)
                                                                             agcagcTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATccc                                              <  2:336433/139‑1 (MQ=255)
                                                                             agcagcTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATccc                                              >  1:523008/1‑139 (MQ=255)
                                                                                  cTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTAc                                         >  1:227779/1‑139 (MQ=255)
                                                                                  cTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTAc                                         >  2:21266/1‑139 (MQ=255)
                                                                                   tGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTAcc                                        <  1:296167/139‑1 (MQ=255)
                                                                                    gCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTAccc                                       >  1:789684/1‑139 (MQ=255)
                                                                                         aggtaTTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCa                                  >  2:275441/1‑139 (MQ=255)
                                                                                         aggtaTTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCa                                  <  2:367622/139‑1 (MQ=255)
                                                                                           gtaTTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATAcg                                                                                                            >  1:564678/1‑63 (MQ=255)
                                                                                           gtaTTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATAcg                                                                                                            <  2:564678/63‑1 (MQ=255)
                                                                                                   aTCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATc                        <  1:275441/139‑1 (MQ=255)
                                                                                                        cgcACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAggg                   <  2:166711/139‑1 (MQ=255)
                                                                                                                  aGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGAc         <  2:436321/139‑1 (MQ=255)
                                                                                                                      tGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACt     <  2:523008/139‑1 (MQ=255)
                                                                                                                      tGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACt     <  2:227779/139‑1 (MQ=255)
                                                                                                                       gcgcAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGc                                                                                          <  1:819686/53‑1 (MQ=255)
                                                                                                                       gcgcAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGc                                                                                          >  2:819686/1‑53 (MQ=255)
                                                                                                                       gcgcAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCttttt                                                                     >  2:456474/1‑74 (MQ=255)
                                                                                                                       gcgcAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCttttt                                                                     <  1:456474/74‑1 (MQ=255)
                                                                                                                       gcgcAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCTCAAAAATCCCCCTACCCCGTCa                                  <  2:182511/109‑1 (MQ=255)
                                                                                                                       gcgcAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCTCAAAAATCCCCCTACCCCGTCa                                  >  1:182511/1‑109 (MQ=255)
                                                                                                                       gcgcAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTa    >  2:474120/1‑139 (MQ=255)
                                                                                                                       gcgcAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTa    >  2:126676/1‑139 (MQ=255)
                                                                                                                         gcAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTAtt  >  1:84144/1‑139 (MQ=255)
                                                                                                                                   |                                                                                                                                
GTCCAGCAAGCCGCGCGCGATGCCGTTGGCTGCGCGGATTCTTTTGTTCGTGAAAGACCCTGGTGTAGCGTGGGTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTATT  >  NC_000913/2800344‑2800603

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: