Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,842,931	T→C	D38G (GAC→GGC)	hycI ←	protease involved in processing C‑terminal end of HycE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,842,931	0	T	C	100.0%	123.2 / NA	35	D38G (GAC→GGC)	hycI	protease involved in processing C‑terminal end of HycE
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (20/15);  total (20/15)

CTCGGCGATATCATCCGGGTCGATGATGCGGATCTCGCCGGGGTTTAGCCCCATATCCGTGGCGTCGACAATCAGCAGTCGTGTCGGGCGCAGTTCACGGATAGCGACGATGTCGTTTTCCGGTGCGCTACCGCCGTCAATCACCACCCAGTTACCTTTCGGCGCGGCGGCGCACTTTTCCGCCAGCAGCGGACCTGCGCCATCATCGCCCATCATGCTATTGCCAACACAGAGTAAAACGTCAGTCACGC  >  NC_000913/2842795‑2843045                                                                                                                                         |                                                                                                                   ctcGGCGATATCATCCGGGTCGATGATGCGGATCTCGCCGGGGTTTAGCCCCATATCCGTGGCGTCGACAATCAGCAGTCGTGTCGGGCGCAGTTCACGGATAGCGACGATGTCGTTTTCCGGTGCGCTACCGCCGCCa                                                                                                                  >  2:718711/1‑139 (MQ=255)  tcGGCGATATCATCCGGGTCGATGATGCGGATCTCGCCGGGGTTTAGCCCCATATCCGTGGCGTCGACAATCAGCAGTCGTGTCGGGCGCAGTTCACGGATAGCGACGATGTCGTTTTCCGGTGCGCTACCGCCGCCaa                                                                                                                 >  1:670318/1‑139 (MQ=255)   cGGCGATATCATCCGGGTCGATGATGCGGATCTCGCCGGGGTTTAGCCCCATATCCGTGGCGTCGCCAATCAGCAGTCGTGTCGGGCGCAGTTCACGGATAGCGACGCTGTCGTTTTCCGGTGCGCTACCGCCGCCAAt                                                                                                                >  1:603135/1‑139 (MQ=255)                   gTCGATGATGCGGATCTCGCCGGGGTTTAGCCCCATATCCGTGGCGTCGACAATCAGCAGTCGTGTCGGGCGCAGTTCACGGATAGCGACGATGTCGTTTTCCGGTGCGCTACCGCCGCCAATCACCACCCAGTTACCt                                                                                                >  2:799893/1‑139 (MQ=255)                       atgatgCGGATCTCGCCGGGGTTTAGCCCCATATCCGTGGCGTCGACAATCAGCAGTCGTGTCGGGCGCAGTTCACGGATAGCGACGATGTCGTTTTCCGGTGCGCTACCGCCGCCAATCACCACCCAGTTACCTTTcg                                                                                            >  2:343076/1‑139 (MQ=255)                             cGGATCTCGCCGGGGTTTAGCCCCATATCCGTGGCGTCGACAATCAGCAGTCGTGTCGGGCGCAGTTCGCGGATAGCGACGATGTCGTTTTCCGGTGCGCTACCGCCGCCAATCACCACCCAGTTACCTTTcggcgcgg                                                                                      >  1:521954/1‑139 (MQ=255)                             cGGATCTCGCCGGGGTTTAGCCCCATATCCGTGGCGTCGACAATCAGCAGTCGTGTCGGGCGCAGTTCACGGATAGCGACGATGTCGTTTTCCGGTGCGCTACCGCCGCCAATCACCACCCAGTTACCTTTcggcgcgg                                                                                      <  2:521954/139‑1 (MQ=255)                                      ccGGGGTTTAGCCCCATATCCGTGGCGTCGACAATCAGCAGTCGTGTCGGGCGCAGTTCACGGATAGCGACGATGTCGTTTTCCGGTGCGCTACCGCCGCCAATcac                                                                                                             <  2:366769/107‑1 (MQ=255)                                      ccGGGGTTTAGCCCCATATCCGTGGCGTCGACAATCAGCAGTCGTGTCGGGCGCAGTTCACGGATAGCGACGATGTCGTTTTCCGGTGCGCTACCGCCGCCAATcac                                                                                                             >  1:366769/1‑107 (MQ=255)                                                   ccATATCCGTGGCGTCGACAATCAGCAGTCGTGTCGGGCGCAGTTCACGGATAGCGACGATGTCGTTTTCCGGTGCGCTACCGCCGCCAATCACCACCCAGTTACCTTTCGGCGCGGCGGCGCACTTTTCCGCcagcag                                                                <  2:670318/139‑1 (MQ=255)                                                     atatCCGTGGCGTCGACAATCAGCAGTCGTGTCGGGCGCAGTTCACGGATAGCGACGATGTCGTTTTCCGGTGCGCTACCGCCGCCAATCACCACCCAGTTACCTTTCGGCGCGGCCGCGCACTTTTCCGCCAGCAGCg                                                              >  2:608565/1‑139 (MQ=255)                                                              gCGTCGACAATCAGCAGTCGTGTCGGGCGCAGTTCACGGATAGCGACGATGTCGTTTTCCGGTGCGCTACCGCCGCCAAt                                                                                                                >  2:31227/1‑80 (MQ=255)                                                              gCGTCGACAATCAGCAGTCGTGTCGGGCGCAGTTCACGGATAGCGACGATGTCGTTTTCCGGTGCGCTACCGCCGCCAAt                                                                                                                <  1:31227/80‑1 (MQ=255)                                                                 tCGACAATCAGCAGTCGTGTCGGGCGCAGTTCACGGATAGCGACGATGTCGTTTTCCGGTGCGCTACCGCCGCCAAt                                                                                                                >  1:95561/1‑77 (MQ=255)                                                                 tCGACAATCAGCAGTCGTGTCGGGCGCAGTTCACGGATAGCGACGATGTCGTTTTCCGGTGCGCTACCGCCGCCAAt                                                                                                                <  2:95561/77‑1 (MQ=255)                                                                       aTCAGCAGTCGTGTCGGGCGCAGTTCACGGATAGCGACGATGTCGTTTTCCGGTGCGCTACCGCCGCCAATCACCACCCAGTTACCTTTCGGCGCGGCGGCGCACTTTTCCGCCAGCAGCGGACCTGCGCCATCATCGc                                            <  1:799893/139‑1 (MQ=255)                                                                               tCGTGTCGGGCGCAGTTCACGGATAGCGACGATGTCGTTTTCCGGTGCGCTACCGCCGCCAATCACCACCCAGTTACCTTTCGGCGCGGCGGCGCACTTTTCCGCCAGCAGCGGACCTGCGCCATCATCGCCCATCATg                                    >  1:585475/1‑139 (MQ=255)                                                                                 gtgtCGGGCGCAGTTCACGGATAGCGACGATGTCGTTTTCCGGTGCGCTACCGCCGCCAATCACCACCCAGTTACCTTTCGGCGCGGCGGCGCACTTTTCCGCCAGCAGCGGACCTgcg                                                      >  1:304423/1‑119 (MQ=255)                                                                                 gtgtCGGGCGCAGTTCACGGATAGCGACGATGTCGTTTTCCGGTGCGCTACCGCCGCCAATCACCACCCAGTTACCTTTCGGCGCGGCGGCGCACTTTTCCGCCAGCAGCGGACCTgcg                                                      >  1:29692/1‑119 (MQ=255)                                                                                 gtgtCGGGCGCAGTTCACGGATAGCGACGATGTCGTTTTCCGGTGCGCTACCGCCGCCAATCACCACCCAGTTACCTTTCGGCGCGGCGGCGCACTTTTCCGCCAGCAGCGGACCTgcg                                                      <  2:29692/119‑1 (MQ=255)                                                                                 gtgtCGGGCGCAGTTCACGGATAGCGACGATGTCGTTTTCCGGTGCGCTACCGCCGCCAATCACCACCCAGTTACCTTTCGGCGCGGCGGCGCACTTTTCCGCCAGCAGCGGACCTgcg                                                      <  2:304423/119‑1 (MQ=255)                                                                                  tgtCGGGCGCAGTTCACGGATAGCGACGATGTCGTTTTCCGGTGCGCTACCGCCGCCAATCACCACCCAg                                                                                                      <  1:230618/70‑1 (MQ=255)                                                                                  tgtCGGGCGCAGTTCACGGATAGCGACGATGTCGTTTTCCGGTGCGCTACCGCCGCCAATCACCACCCAg                                                                                                      >  2:230618/1‑70 (MQ=255)                                                                                      gggCGCAGTTCACGGATAGCGACGATGTCGTTTTCCGGTGCGCTACCGCCGCCAATCACCACCCAGTTACCTTTCGGCGCGGCGGCGCACTTTTCCGCCAGCAGCGGACCTGCGCCATCATCGCCCATCATGCTATTGc                             <  2:196351/139‑1 (MQ=255)                                                                                       ggCGCAGTTCACGGATAGCGACGATGTCGTTTTCCGGTGCGCTACCGCCGCCAATCACCACCCAGTTACCTTTCGGCGCGGCGGCGCACTTTTCCGCCAGCAGCGGACCTGCGCCATCATCGCCCATCATGCTATTGcc                            >  2:807891/1‑139 (MQ=255)                                                                                         cgcAGTTCACGGATAGCGACGATGTCGTTTTCCGGTGCGCTACCGCCGCCAATCACCACCCAGTTACCTTTCGGCGCGGCGGCGCACTTTTCCGCCAGCAGCGGACCTGCGCCATCATCg                                             >  1:694591/1‑120 (MQ=255)                                                                                         cgcAGTTCACGGATAGCGACGATGTCGTTTTCCGGTGCGCTACCGCCGCCAATCACCACCCAGTTACCTTTCGGCGCGGCGGCGCACTTTTCCGCCAGCAGCGGACCTGCGCCATCATCg                                             <  2:694591/120‑1 (MQ=255)                                                                                          gcAGTTCACGGATAGCGACGATGTCGTTTTCCGGTGCGCTACCGCCGCCAATCACCACCCAGTTACCTTTCGGCGCGGCGGCGCACTTTTCCGCCAGCAGCGGACCTGCGCCATCATCGCCCATCATGCTATTGCCAac                         <  1:537340/139‑1 (MQ=255)                                                                                              ttCACGGATAGCGACGATGTCGTTTTCCGGTGCGCTACCGCCGCCAATCaccacc                                                                                                         <  2:497469/55‑1 (MQ=255)                                                                                              ttCACGGATAGCGACGATGTCGTTTTCCGGTGCGCTACCGCCGCCAATCaccacc                                                                                                         >  1:497469/1‑55 (MQ=255)                                                                                                cACGGATAGCGACGATGTCGTTTTCCGGTGCGCTACCGCCGCCAATCACCACCCAGTTa                                                                                                   <  2:224568/59‑1 (MQ=255)                                                                                                cACGGATAGCGACGATGTCGTTTTCCGGTGCGCTACCGCCGCCAATCACCACCCAGTTa                                                                                                   >  1:224568/1‑59 (MQ=255)                                                                                                        gCGACGATGTCGTTTTCCGGTGCGCTACCGCCGCCAATCACCACCCAGTTACCTTTCGGCGCGGCGGCGCACTTTTCCGCCAGCAGCGGACCTGCGCCTTCATCGCCCATCATGCTATTGCCAACACAGAGTAAAACgt           >  2:428108/1‑139 (MQ=255)                                                                                                        gCGACGATGTCGTTTTCCGGTGCGCTACCGCCGCCAATCACCACCCAGTTACCTTTCGGCGCGGCGGCGCACTTTTCCGCCAGCAGCGGACCTGCGCCATCATCGCCCATCATGCTATTGCCAACACAGAGTAAAACgt           >  1:594207/1‑139 (MQ=255)                                                                                                                 tCGTTTTCCGGTGCGCTACCGCCGCCAATCACCACCCAGTTACCTTTCGGCGCGGCGGCGCACTTTTCCGCCAGCAGCGGACCTGCGCCATCATCGCCCATCATGCTATTGCCAACACAGAGTAAAACGTCAGTCACGc  <  2:585475/139‑1 (MQ=255)                                                                                                                                         |                                                                                                                   CTCGGCGATATCATCCGGGTCGATGATGCGGATCTCGCCGGGGTTTAGCCCCATATCCGTGGCGTCGACAATCAGCAGTCGTGTCGGGCGCAGTTCACGGATAGCGACGATGTCGTTTTCCGGTGCGCTACCGCCGTCAATCACCACCCAGTTACCTTTCGGCGCGGCGGCGCACTTTTCCGCCAGCAGCGGACCTGCGCCATCATCGCCCATCATGCTATTGCCAACACAGAGTAAAACGTCAGTCACGC  >  NC_000913/2842795‑2843045

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑