Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,993,856 G→A *73* (TAG→TAA ygeI → uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,993,8560GA100.0% 88.3 / NA 29*73* (TAG→TAAygeIuncharacterized protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (16/13);  total (16/13)

CACCAGTGCTACTGATACAGATCAAATACAAGCATTTATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTAT  >  NC_000913/2993733‑2993985
                                                                                                                           |                                                                                                                                 
cacCAGTGCTACTGATACAGATCAAATACAAGCATTTATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGACCaaata                                                                                                                    <  1:66155/139‑1 (MQ=255)
       gCTACTGATACAGATCAAATACAAGCATTTATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCATa                                                                                                             >  2:429677/1‑139 (MQ=255)
               tACAGATCAAATACAAGCATTTATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCATAACGAGATa                                                                                                     <  1:762161/139‑1 (MQ=255)
                aCAGATCAAATACAAGCATTTATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCATAACGAGATAc                                                                                                    <  1:429677/139‑1 (MQ=255)
                    aTCAAATACAAGCATTTATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCATAACGAGATACtttt                                                                                                >  2:51623/1‑139 (MQ=255)
                         aTACAAGCATTTATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCATAACGAGATACTTTTctaat                                                                                           >  2:208664/1‑139 (MQ=255)
                           aCAAGCATTTATAGTTTCAACATGGATGGCGCCTTTTCAAAATGTTATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAg                                                                                         >  2:252622/1‑139 (MQ=255)
                                   ttATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGt                                                                                 >  2:82541/1‑139 (MQ=255)
                                           tCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAAATAATCCTATCTCACCTTACTATAAAATTGAGAGGTAAACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTttca                                                                         >  2:589736/1‑139 (MQ=255)
                                             aaCATGGACGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGCTAAACGATTGACCAAATAAATCATAAAGAGATACTTTTATAATAGTATCTCGTTCCTttcatt                                                                       <  2:545135/139‑1 (MQ=255)
                                              aCATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTttcattc                                                                      <  1:175232/139‑1 (MQ=255)
                                                       gcgcCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGACCaaat                                                                                                                     <  2:370025/83‑1 (MQ=255)
                                                       gcgcCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGACCaaat                                                                                                                     >  1:370025/1‑83 (MQ=255)
                                                                       atatGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCATaa                                                                                                            <  1:691378/76‑1 (MQ=255)
                                                                       atatGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCATaa                                                                                                            >  2:691378/1‑76 (MQ=255)
                                                                               tCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTttcatt                                                                       >  2:127873/1‑105 (MQ=255)
                                                                               tCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTttcatt                                                                       <  1:127873/105‑1 (MQ=255)
                                                                                     gaTAATCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGtt                               <  1:51623/139‑1 (MQ=255)
                                                                                     gaTAATCCTAACTCACCTTACTATAAAATTGAGTGGTAAACGATTGATCAAATAAATAATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGtt                               <  1:283729/139‑1 (MQ=255)
                                                                                      aTAATCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCaa                                    <  2:834633/133‑1 (MQ=255)
                                                                                      aTAATCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCaa                                    >  1:834633/1‑133 (MQ=255)
                                                                                      aTAATCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTa                              >  2:180811/1‑139 (MQ=255)
                                                                                      aTAATCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTa                              >  1:679170/1‑139 (MQ=255)
                                                                                        aaTCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGACCAAATAAAACATAACGAGATACTTTta                                                                                               <  2:474439/72‑1 (MQ=255)
                                                                                        aaTCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGACCAAATAAAACATAACGAGATACTTTta                                                                                               >  1:474439/1‑72 (MQ=255)
                                                                                                   aCCTTACTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCATAACgag                                                                                                        >  2:350182/1‑52 (MQ=255)
                                                                                                   aCCTTACTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCATAACgag                                                                                                        <  1:350182/52‑1 (MQ=255)
                                                                                                   aCCTTACTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCATAAAGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCAt                 >  2:667695/1‑139 (MQ=255)
                                                                                                                  tGAGTGGTAAACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGtat  >  2:358813/1‑139 (MQ=255)
                                                                                                                           |                                                                                                                                 
CACCAGTGCTACTGATACAGATCAAATACAAGCATTTATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTAT  >  NC_000913/2993733‑2993985

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: