Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,104,065	G→A	*351* (TAG→TAA)	mutY →	adenine DNA glycosylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,104,065	0	G	A	100.0%	121.8 / NA	39	*351* (TAG→TAA)	mutY	adenine DNA glycosylase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (19/20);  total (19/20)

TTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAGCGCGTGAGTCGATAAAGAGGATGATTTATGAGCAGAACGATTTTTTGTACTTTCCTGCAACGTGAAGCAGAAGGTCAGGATTTTCAGCTGTACCCCGGCGAGCTGGGAAAACGCATCTATAACGAGATCT  >  NC_000913/3103929‑3104195                                                                                                                                         |                                                                                                                                   ctCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAacg                                                                                                                                  >  2:524257/2‑139 (MQ=255)    ccGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAACGCGt                                                                                                                               >  2:364559/1‑139 (MQ=255)                tCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAACGCGTGAGTCGATAAag                                                                                                                   <  1:364559/139‑1 (MQ=255)                    cGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAACGCGTGAGTCGATAAAGAGga                                                                                                               >  2:89771/1‑139 (MQ=255)                     ggCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAACGCGTGAGTCGATAAAGAGgat                                                                                                              <  1:640185/139‑1 (MQ=255)                          cATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAACGCGTGAGTCGATAAAGAGGATGAttt                                                                                                         >  1:419382/1‑139 (MQ=255)                               aTGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAACGCGTGAGTCGATAAAGAGGATGATTTATGAg                                                                                                    <  2:292929/139‑1 (MQ=255)                                         tGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAACGCGTGAGTCGATAAAGAGgat                                                                                                              >  1:702885/1‑119 (MQ=255)                                         tGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAACGCGTGAGTCGATAAAGAGgat                                                                                                              <  2:702885/119‑1 (MQ=255)                                         tGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAACGCGTGAGTCGATAAAGAGGATGATTTATGAGCAGAACGAtt                                                                                          >  1:309628/1‑139 (MQ=255)                                             ctctGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAACGCGTGAGTCGATAAAGAGGATGATTTATGAGCAGAACGAtttttt                                                                                      >  2:293674/1‑139 (MQ=255)                                                            gcgcAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAACGCGTGAg                                                                                                                            >  2:603945/1‑86 (MQ=255)                                                            gcgcAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAACGCGTGAg                                                                                                                            <  1:603945/86‑1 (MQ=255)                                                            gcgcAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAACGCGTGAGTCGATAAAGAGgat                                                                                                              >  2:671937/1‑100 (MQ=255)                                                            gcgcAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAACGCGTGAGTCGATAAAGAGgat                                                                                                              <  1:671937/100‑1 (MQ=255)                                                            gcgcAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAACGCGTGAGTCGATAAAGAGGATGATTTATGAGCAGAACGATTTTTTGTACTTTCCTGCAAc                                                                       >  2:83713/1‑139 (MQ=255)                                                            gcgcAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAACGCGTGAGTCGATAAAGAGGATGATTTATGAGCAGAACGATTTTTTGTACTTTCCTGCAAc                                                                       <  1:89771/139‑1 (MQ=255)                                                            gcgcAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAACGCGTGAGTCGATAAAGAGGATGATTTATGAGCAGAACGATTTTTTGTACTTTCCTGCAAc                                                                       >  1:465311/1‑139 (MQ=255)                                                            gcgcAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAACGCGTGAGTCGATAAAGAGGATGATTTATGAGCAGAACGATTTTTTGTACTTTCCTGCAAc                                                                       >  2:284604/1‑139 (MQ=255)                                                             cgcAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAAcgcg                                                                                                                                >  2:17563/1‑81 (MQ=255)                                                             cgcAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAAcgcg                                                                                                                                <  1:17563/81‑1 (MQ=255)                                                             cgcAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAACGCGTGAGTCGATAAAGAGGATGATTTATGAGCAGAACGATTTTTTGTACTTTCCTGCAACg                                                                      >  1:215558/1‑139 (MQ=255)                                                             cgcAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAACGCGTGAGTCGATAAAGAGGATGATTTATGAGCAGAACGATTTTTTGTACTTTCCTGCAACg                                                                      <  2:309628/139‑1 (MQ=255)                                                             cgcAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAACGCGTGAGTCGATAAAGAGGATGATTTATGAGCAGAACGATTTTTTGTACTTTCCTGCAACg                                                                      <  1:689212/139‑1 (MQ=255)                                                                              ggCCTAGCGGCTCCCGTGGAGCGTTTGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAACGCGTGAGTCGATAAAGAGGATGATTTATGAGCAGAACGATTTTTTGTACTTTCCTGCAACTTGAAGCAGAAGGTCAgg                                                     <  1:524257/139‑1 (MQ=255)                                                                                                     tttGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAACGCGTGAGTCGATAAAGAGGATGATTTATGAGCAGAACGATTTTTTGTACTTTCCTg                                                                           >  2:50371/1‑94 (MQ=255)                                                                                                     tttGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAACGCGTGAGTCGATAAAGAGGATGATTTATGAGCAGAACGATTTTTTGTACTTTCCTg                                                                           <  1:50371/94‑1 (MQ=255)                                                                                                     tttGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAACGCGGGAGTCGATAAAGAGGATGATTTATGAGCAGAACGATTTTTTGTACTTTCCTGCACCGTGAAGCAGAAGGGCAGGATATTCAGCTGGACCCCGGctgg                              >  2:809846/1‑136 (MQ=255)                                                                                                           aCAGCAGTTACGCACTGGCGCGCCGGTTTAACGCGTGAGTCGATAAAGAGGATGATTTATGAGCAGAACGATTTTTTGTACTTTCCTGCAACGTGAAGCAGAAgg                                                          >  2:617603/1‑105 (MQ=255)                                                                                                           aCAGCAGTTACGCACTGGCGCGCCGGTTTAACGCGTGAGTCGATAAAGAGGATGATTTATGAGCAGAACGATTTTTTGTACTTTCCTGCAACGTGAAGCAGAAgg                                                          <  1:617603/105‑1 (MQ=255)                                                                                                              gcagTTACGCACTGGCGCGCCGGTTTAACGCGTGAGTCGATAAAGAGGATGATTTATGAGCAGAACGATTTTTTGTACTTTCCTGCAACGTGAAGCAGAAGGTCAGGATTTTCAGCTGTACCCCGGCGAGCTGGGaaaa                     <  2:465311/139‑1 (MQ=255)                                                                                                               cagTTACGCACTGGCGCGCCGGTTTAACGCGTGAGTCGATAAAGAGGATGATTTATGAGCAGAACGATTTTTTGTACTTTCCTGCAACGTGAAGCAGAAGGTCAGGATTTTCAGCTGTACCCCGGCGAGCTGGGAAAAc                    <  1:62468/139‑1 (MQ=255)                                                                                                                 gTTACGCACTGGCGCGCCGGTTTAACGCGTGAGTCGATAAAGAGGATGATTTATGAGCAGAACGATTTTTTGTACTTTCCTGCAACGTGAAGCAGAAGGTCAGGATTTTCAGCTGTACCCCGGCGAGCTGGGAAAACGc                  <  2:342853/139‑1 (MQ=255)                                                                                                                            gcgcgcCGGTTTAACGCGTGAGTCGATAAAGAGGATGATTTATGAGCAGAACGATTTTTTGTACTTTCCTGCAACGTGAAGCAGAAGGTCAGGATTTTCAGCTGTACCCCGGCGAGCTGGGAAAACGCATCTATAACga       >  2:598814/1‑139 (MQ=255)                                                                                                                             cgcgcCGGTTTAACGCGTGAGTCGATAAAGAGGATGATTTATGAGCAGAACGATTTTTTGTACTTTCCTGCAACGTGAAGCAGAAGGTCAGGATTTTCAGCTGTACCCCGGCGAGCTGGGAAAACGCATCTATAACgag      <  2:215558/139‑1 (MQ=255)                                                                                                                                 ccGGTTTAACGCGTGAGTCGATAAAGAGGATGATTTATGAGCAGAACGATTTTTTGTACTTTCCTg                                                                           <  1:629323/66‑1 (MQ=255)                                                                                                                                 ccGGTTTAACGCGTGAGTCGATAAAGAGGATGATTTATGAGCAGAACGATTTTTTGTACTTTCCTg                                                                           >  2:629323/1‑66 (MQ=255)                                                                                                                                 ccGGTTTAACGCGTGAGTCGATAAAGAGGATGATTTATGAGCAGAACGATTTTTTGTACTTTCCTGCAACGTGAAGCAGAAGGTCAGGATTTTCAGCTGTACCCCGGCGAGCTGGGAAAACGCATCTATAACGAGAtct  <  1:284604/139‑1 (MQ=255)                                                                                                                                    gTTTAACGCGTGAGTCGATAAAGAGGATGATTTATGAGCAGAACGATTTTTTGTACTTTCCTGCAACGTGAAGCAGAAGGTCAGGATTTTCAGCTGTACCCCGGCGAGCt                            >  2:837063/1‑110 (MQ=255)                                                                                                                                    gTTTAACGCGTGAGTCGATAAAGAGGATGATTTATGAGCAGAACGATTTTTTGTACTTTCCTGCAACGTGAAGCAGAAGGTCAGGATTTTCAGCTGTACCCCGGCGAGCt                            <  1:837063/110‑1 (MQ=255)                                                                                                                                         |                                                                                                                                   TTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAGCGCGTGAGTCGATAAAGAGGATGATTTATGAGCAGAACGATTTTTTGTACTTTCCTGCAACGTGAAGCAGAAGGTCAGGATTTTCAGCTGTACCCCGGCGAGCTGGGAAAACGCATCTATAACGAGATCT  >  NC_000913/3103929‑3104195

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑