Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,271,932 T→C T274A (ACG→GCG)  garR ← tartronate semialdehyde reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,271,9320TC100.0% 108.6 / NA 31T274A (ACG→GCG) garRtartronate semialdehyde reductase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (15/16);  total (15/16)

CTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGAAGTATCCAGCGCATTCGCCAGATCCTTAATATGCAGAT  >  NC_000913/3271808‑3272051
                                                                                                                            |                                                                                                                       
ctctTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGCTCCTAAACCATCTg                                                                                                           <  1:770225/139‑1 (MQ=255)
   ttATTCGGCCTGGATTCCAGGCTCGCATTGCAATACGCCAGCCGGCCACGACGTCATCAATGTGTAATGTCGATTTTCGCCTGTCCATCGTAGTAGCACTCGAGGGCGCTATGATCGGCCGCTCCTAAACCATCTGCTc                                                                                                        >  2:833887/1‑139 (MQ=255)
           ccTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGCTCCTAAACCATCTGCTCGCAGTGcc                                                                                                >  1:717071/1‑139 (MQ=255)
           ccTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGCTCCTAAACCATCTGCTCGCAGTGcc                                                                                                >  2:225798/1‑139 (MQ=255)
                         ccGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGCTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCAt                                                                                  <  1:546628/139‑1 (MQ=255)
                                       gCCATCCGGGCACGACGTCATTAAGGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGCTCCTAAACCATCTGCTCGCGGTGCCTGCATCATCTCCATAACCGCAGCTGTGa                                                                    >  2:569188/1‑139 (MQ=255)
                                       gCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGCTCCTAAACCATCTGCTCGCAGTGCCTGcatca                                                                                         >  2:840614/1‑118 (MQ=255)
                                       gCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGCTCCTAAACCATCTGCTCGCAGTGCCTGcatca                                                                                         <  1:840614/118‑1 (MQ=255)
                                         cATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGCTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGc                                                                  >  2:778925/1‑139 (MQ=255)
                                               ggCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGCTCCTAAACCATCTGCTCGCAGTGCCTGcatc                                                                                          <  1:302165/109‑1 (MQ=255)
                                               ggCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGCTCCTAAACCATCTGCTCGCAGTGCCTGcatc                                                                                          >  2:302165/1‑109 (MQ=255)
                                                     acgTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGCTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTgcg                                                      >  2:101402/1‑139 (MQ=255)
                                                              aCGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGCTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCg                                             >  2:427456/1‑139 (MQ=255)
                                                               cGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGCTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGt                                            <  2:717071/139‑1 (MQ=255)
                                                                   tAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGCTCCTAAACCATCTGCTCGCAg                                                                                                    <  2:840800/79‑1 (MQ=255)
                                                                   tAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGCTCCTAAACCATCTGCTCGCAg                                                                                                    >  1:840800/1‑79 (MQ=255)
                                                                                      tttttCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGCTCCTAAACCAGCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGAAGTATCCAGCGCATTCGcc                     <  2:566129/139‑1 (MQ=255)
                                                                                      tttttCGTAGTAGCACACCAGGGCGCTATGATCCGCCGCTCCTAAACCATCTGCTCGCGGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGACAAGTATCCAGCGCATTCGcc                     <  1:569188/139‑1 (MQ=255)
                                                                                       ttttCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGCTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGAAGTATCCAGCGCATTCGCCa                    <  1:225798/139‑1 (MQ=255)
                                                                                                tagCACGCCAGGGCGCTATGATCCGCCGCTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGTAGTTGTGCGCCGACGc                                               >  1:554802/1‑103 (MQ=255)
                                                                                                tagCACGCCAGGGCGCTATGATCCGCCGCTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGc                                               <  2:554802/103‑1 (MQ=255)
                                                                                                tagCACGCCAGGGCGCTATGATCCGCCGCTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGAAGTATCCAGCGCATTCGCCAGATCCTTaa           >  2:436263/1‑139 (MQ=255)
                                                                                                  gCACGCCAGGGCGCTATGATCCGCCGCTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGAAGTATCCAGCGCATTCGCCAGATCCTTAata         <  1:427456/139‑1 (MQ=255)
                                                                                                     cGCCAGGGCGCTATGATCCGCCGCTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCtgtg                                                                     >  1:663146/1‑76 (MQ=255)
                                                                                                     cGCCAGGGCGCTATGATCCGCCGCTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCtgtg                                                                     <  2:663146/76‑1 (MQ=255)
                                                                                                      gCCAGGGCGCTATGATCCGCCGCTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGAAGTATCCAGCGCATTCGCCAGATCCTTAATATGCa     <  1:47060/139‑1 (MQ=255)
                                                                                                         aGGGCGCTATGATCCGCCGCTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGGGAAGTATCCAGCGCATTCGCCAGATCCTTAATATGCAGAt  <  1:778925/139‑1 (MQ=255)
                                                                                                                tATGATCCGCCGCTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGAAGTATCCAGCGCATTCGCCAGAt                 <  1:647348/117‑1 (MQ=255)
                                                                                                                tATGATCCGCCGCTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGAAGTATCCAGCGCATTCGCCAGAt                 >  2:647348/1‑117 (MQ=255)
                                                                                                                    aTCCGCCGCTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAg                                                                   >  2:291037/1‑63 (MQ=255)
                                                                                                                    aTCCGCCGCTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAg                                                                   <  1:291037/63‑1 (MQ=255)
                                                                                                                            |                                                                                                                       
CTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGAAGTATCCAGCGCATTCGCCAGATCCTTAATATGCAGAT  >  NC_000913/3271808‑3272051

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: