Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,308,040 C→T *295* (TAG→TAA nlpI ← lipoprotein involved in osmotic sensitivity and filamentation

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,308,0400CT100.0% 50.1 / NA 17*295* (TAG→TAAnlpIlipoprotein involved in osmotic sensitivity and filamentation
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (7/10);  total (7/10)

AATTGATCAGCAATTTTCATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAAC  >  NC_000913/3307914‑3308144
                                                                                                                              |                                                                                                        
aaTTGATCAGCAATTTTCATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTcc                                                                                              >  2:446384/1‑139 (MQ=255)
     aTCAGCAATTTTCATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGc                                                                                                    <  1:591835/128‑1 (MQ=255)
     aTCAGCAATTTTCATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGc                                                                                                    >  2:591835/1‑128 (MQ=255)
       cAGCAATTTTCATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTg                                                                                       <  1:779614/139‑1 (MQ=255)
                                       cAAAGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGAt                                                       <  2:646138/139‑1 (MQ=255)
                                           gTAGGTGTATTAACGAACAACACCGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGTGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAAtt                                                   <  2:742213/139‑1 (MQ=255)
                                                                               tAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGAt                                                       <  2:158200/99‑1 (MQ=255)
                                                                               tAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGAt                                                       >  1:158200/1‑99 (MQ=255)
                                                                                        ggCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGAtt                                                                                          >  1:518944/1‑55 (MQ=255)
                                                                                        ggCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGAtt                                                                                          <  2:518944/55‑1 (MQ=255)
                                                                                        ggCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCt                                                                            >  2:177141/1‑69 (MQ=255)
                                                                                        ggCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCt                                                                            <  1:177141/69‑1 (MQ=255)
                                                                                            aTCAAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAAc  <  2:200375/139‑1 (MQ=255)
                                                                                                   aaGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGAt                                                       >  2:435857/1‑79 (MQ=255)
                                                                                                   aaGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGAt                                                       <  1:435857/79‑1 (MQ=255)
                                                                                                                gATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCAt                                                                              <  1:713751/43‑1 (MQ=255)
                                                                                                                gATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCAt                                                                              >  2:713751/1‑43 (MQ=255)
                                                                                                                              |                                                                                                        
AATTGATCAGCAATTTTCATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAAC  >  NC_000913/3307914‑3308144

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: