Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,340,588	T→C	L105S (TTA→TCA)	yrbG →	putative calcium/sodium:proton antiporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,340,588	0	T	C	100.0%	192.5 / NA	54	L105S (TTA→TCA)	yrbG	putative calcium/sodium:proton antiporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (28/26);  total (28/26)

CGTCTCTGCACGAACAACGCGATTTAGCCGTTGGTACAGCCCTCGGCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATTACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTACTCTATGACGGACAACTTAGTCGCAGCGATGGTATCTTTCTCCTCTTTCTGGCTGTGCTATGGCTGCTGTTCATTGTTAAACTTGCACGTCAG  >  NC_000913/3340453‑3340721                                                                                                                                        |                                                                                                                                      cgTCTCTGCACGAACAACGTGATTTAGCCGTTTGTACAGCCCTCGGCTCCAACATTCTCAATTTATTGCTTTTCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACCGTTCATTCTGCTGTTCTACGCCGTGAATCAcc                                                                                                                                    <  2:225674/139‑1 (MQ=255)                 acGCGATTTAGCCGTTGGTACAGCCCTCGGCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATCACCCTTaa                                                                                                                               >  1:156267/1‑128 (MQ=255)                 acGCGATTTAGCCGTTGGTACAGCCCTCGGCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATCACCCTTaa                                                                                                                               <  2:156267/128‑1 (MQ=255)                       tttAGCCGTTGGTACAGCCCTCGGCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCgtg                                                                                                              <  2:98392/139‑1 (MQ=255)                            ccGTTGGTACAGCCCTCGGCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGc                                                                                                         <  1:73619/139‑1 (MQ=255)                               ttGGTACAGCCCTCGGCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCgg                                                                                                      <  2:150739/139‑1 (MQ=255)                                          cTCGGCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCg                                                                                                       >  1:282097/1‑127 (MQ=255)                                          cTCGGCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCg                                                                                                       <  2:282097/127‑1 (MQ=255)                                             ggCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTACTCTATg                                                                                        >  2:303874/1‑139 (MQ=255)                                                                  ttGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTACTCTATGACGGACAACTTAGTCg                                                                        <  2:644629/134‑1 (MQ=255)                                                                  ttGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTACTCTATGACGGACAACTTAGTCg                                                                        >  1:644629/1‑134 (MQ=255)                                                                   tGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTACTCTATGACGGa                                                                                   >  2:708485/1‑122 (MQ=255)                                                                   tGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTACTCTATGACGGa                                                                                   <  1:708485/122‑1 (MQ=255)                                                                    gCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACCTTCCATTCTGATGTTCTACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGtt                                                                                                    <  2:546654/104‑1 (MQ=255)                                                                    gCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGtt                                                                                                    >  1:337913/1‑104 (MQ=255)                                                                    gCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGtt                                                                                                    >  1:546654/1‑104 (MQ=255)                                                                    gCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGtt                                                                                                    <  2:337913/104‑1 (MQ=255)                                                                     cTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTACTCTa                                                                                          >  2:179852/1‑113 (MQ=255)                                                                     cTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTACTCTa                                                                                          <  1:179852/113‑1 (MQ=255)                                                                       gATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTACTCTATGACGGACAACTTAGTCGCAg                                                                     <  1:267698/132‑1 (MQ=255)                                                                       gATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTACTCTATGACGGACAACTTAGTCGCAg                                                                     >  2:267698/1‑132 (MQ=255)                                                                        aTCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACCGTCCATTCCGATGTTCTACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTACTCTATGACGGACAACTTAGTCGCAGCGATGGTa                                                             >  2:594280/1‑139 (MQ=255)                                                                        aTCCTCGGTCTGGCTGCACTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGc                                                                                                         >  2:528854/1‑95 (MQ=255)                                                                        aTCCTCGGTCTGGCTGCACTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGc                                                                                                         <  1:528854/95‑1 (MQ=255)                                                                             cGGTCTGGCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCATGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTACTCTATGACGGACAACTTAGTCGCAGCGATGGTATCttt                                                        >  1:338095/1‑139 (MQ=255)                                                                               gTCTGGCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATCACCCTTAATGTTGTTGGTGAGCGTGGTGGCCGGTTCCGTACTCTATGACg                                                                                     >  2:600575/1‑108 (MQ=255)                                                                               gTCTGGCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTACTCTATGACg                                                                                     <  1:600575/108‑1 (MQ=255)                                                                               gTCTGGCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTACTCTATGACGGACAACTTAGTCGCAGCGATGGTATCTTtct                                                      <  1:136905/139‑1 (MQ=255)                                                                                    gCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCg                                                                                                       >  1:518462/1‑85 (MQ=255)                                                                                    gCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCg                                                                                                       <  2:518462/85‑1 (MQ=255)                                                                                            ggTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTACTCTATGACGGACAACTTAGTCGCAGCGATGGTATCTTTCTCCTCTTTCTGGCt                                         <  1:303874/139‑1 (MQ=255)                                                                                              ttCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGt                                                                                                <  2:572264/82‑1 (MQ=255)                                                                                              ttCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGt                                                                                                >  1:572264/1‑82 (MQ=255)                                                                                               tCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGCATCACCCTTCATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTACTCTATGACGGACAACTTAGTCACAGCGATGGTATCTTTCTCCTCTTTCTGGCtgtg                                      >  2:101226/1‑139 (MQ=255)                                                                                                  tCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGtt                                                                                                    >  2:624413/1‑74 (MQ=255)                                                                                                  tCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGtt                                                                                                    <  1:624413/74‑1 (MQ=255)                                                                                                  tCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTACTCTATGACGGACAACTTAGTCGCAGCGATGGTATCTTTCTCCTCTTTGTGGCTGTGCTa                                   >  2:635295/1‑139 (MQ=255)                                                                                                             tCCATTCTGATGTTCTACGCCGTGAATCACCCTTAATGTTGTTGGTTAGCGTGGTGGCCGGTTCCGTACTCTATg                                                                                        <  1:227804/75‑1 (MQ=255)                                                                                                             tCCATTCTGATGTTCTACGCCGTGAATCACCCTTAATGTTGTTGGTTAGCGTGGTGGCCGGTTCCGTACTCTATg                                                                                        >  2:227804/1‑75 (MQ=255)                                                                                                             tCCATTCTGATGTTCTACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCg                                                                                                       <  2:528221/60‑1 (MQ=255)                                                                                                             tCCATTCTGATGTTCTACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCg                                                                                                       >  1:654490/1‑60 (MQ=255)                                                                                                             tCCATTCTGATGTTCTACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCg                                                                                                       >  1:528221/1‑60 (MQ=255)                                                                                                             tCCATTCTGATGTTCTACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCg                                                                                                       <  2:654490/60‑1 (MQ=255)                                                                                                             tCCATTCTGATGTTCTACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTACTCTATGACGGACAACTTAGTCGCAg                                                                     <  1:690181/94‑1 (MQ=255)                                                                                                             tCCATTCTGATGTTCTACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTACTCTATGACGGACAACTTAGTCGCAg                                                                     >  2:690181/1‑94 (MQ=255)                                                                                                             tCCATTCTGATGTTCTACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTACTCTATGACGGACAACTTAGTCGCAGCGATGGTATCTTTCTCCTCttt                                               <  1:711787/116‑1 (MQ=255)                                                                                                             tCCATTCTGATGTTCTACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTACTCTATGACGGACAACTTAGTCGCAGCGATGGTATCTTTCTCCTCttt                                               >  2:711787/1‑116 (MQ=255)                                                                                                             tCCATTCTGATGTTCTACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTACTCTATGACGGACAACTTAGTCGCAGCGATGGTATCTTTCTCCTCTTTCTGGCTGTGCTATGGCTGCTGtt                        >  1:636140/1‑139 (MQ=255)                                                                                                                 ttCTGATGTTCTACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCg                                                                                                 >  2:203831/1‑62 (MQ=255)                                                                                                                 ttCTGATGTTCTACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCg                                                                                                 <  1:203831/62‑1 (MQ=255)                                                                                                                            tACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTACTCTATGACGGACAACTTAGTCGCAGCGATGGTATCTTTCTCCTCTTTCTGGCTGTGCTATGGCTGCTGTTCATTGTTAAACTTGc         >  2:14280/1‑139 (MQ=255)                                                                                                                                   tGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTACTCTATGACGGACAACTTAGTCGCAGCGATGGTATCTTTCTCCTCTTTCTGGc                                          >  1:820891/1‑99 (MQ=255)                                                                                                                                   tGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTACTCTATGACGGACAACTTAGTCGCAGCGATGGTATCTTTCTCCTCTTTCTGGc                                          <  2:820891/99‑1 (MQ=255)                                                                                                                                   tGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTACTCTATGACGGACAACTTAGTCGCAGCGATGGTATCTTTCTCCTCTTTCTGGCTGTGCTATGGCTGCTGTTCATTGTTAAACTTGCACGTCAg  >  1:451222/1‑139 (MQ=255)                                                                                                                                        |                                                                                                                                      CGTCTCTGCACGAACAACGCGATTTAGCCGTTGGTACAGCCCTCGGCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATTACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTACTCTATGACGGACAACTTAGTCGCAGCGATGGTATCTTTCTCCTCTTTCTGGCTGTGCTATGGCTGCTGTTCATTGTTAAACTTGCACGTCAG  >  NC_000913/3340453‑3340721

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑